Rapgef6 (NM_175258) Mouse Tagged ORF Clone
CAT#: MR215350
- TrueORF®
Rapgef6 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_175258" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Rapgef6 |
Synonyms | A530068K01; AI844632; BB085664; C030018K18Rik; mKIAA4052; PDZ-GEF2; Pdzgef2; RA-GEF-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR215350 representing NM_175258
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCGCCCGTGGACCCCGGCGCTCGGCAGGCACTGAGGAAGAAGCCGCCCGAGCGGACTCCTGAGG ACTTAAATATTATTTATTCTTATCTGCATGGGATGGAAATACTATCAAACCTCAGGGAGCATCAGCTTAG GTTAATGTCTACAAGAGCACGCTATGAGAGATACAGTGGCAATCAAATGCTTTTTTGTTCAGAAACTATT GCCAGATGTTGGTATATCCTGCTTTCTGGATCTGTACTTGTAAAAGATTCTATGGTCCTTCCTCCTTGCA GTTTTGGTAAGCAGTTTGGAGGAAAAAGAGGATGTGATTGTCTTGTGTTAGAACCTTCAGAAATGATTGT GGTAGAGAATTCCAAAGATAATGAAGACAGTATTTTACAAAGAGAAATTCCTGCCAGACAGTCTCGACGA AGGTTTCGGAAAATTAACTATAAAGGAGAACGCCAAACCATAATAGATGGAGTGGATATTAATAACTACC TTTCGCTTCCAGCTGATCTTACCAAGATGCACCTCACAGACAACCCTCATCCACAGGTGACGCATGTGTC TTCTAGTCAGTCTGGCTGTAGCATTGCCAGTGACTCTGGGAGCAGCAGTTTATCCGATATCTATCAGGCT ACAGAGAGTGAAGTAGGAGATGTAGATTTAACACGCCTTCCAGAAGGACCTGTTGATTCTGAGGATGAAG AGGAGGAGGAAGAAGAGATTGATAGAACAGATCCACTGCAGGGTCGAGATCTTGTTCGGGAGTGTCTTGA AAAGGAACCCGCAGACAAAACTGATGATGATGTTGAACAGTTACTTGAGTTCATGCACCAGCTGCCTGCC TTTGCGAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCCGTCATGGTTTTTGAAGTAGTAGAGCAGG CTGGGGCTGTTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTCATTTTAAATGGCACTGTAGA AATTAGTCATCCAGATGGGAAAATAGAAAATCTCTTCATGGGAAATAGTTTTGGGATTGTCCCCACGCTG GATAAGCAGCACATGCATGGAGCTGTCAGGACCAAAGTCGATGATTGTCAGTTTGTCTGCATAGCTCAAC AAGACTATTGGAGAATTTTAAATCATGTGGAAAAAAATACTCACAAAGTTGAGGAAGAGGGAGAGATTGT CATGGTACATGAGCACCGTGAGCTAGACCGGAGCGGAACCAGGAAAGGACACATTGTAATCAAGGCAACA CCTGAGCGTCTCATCATGCATTTGATAGAAGAACATTCTATTGTGGACCCAACATACATAGAAGATTTCC TCTTAACTTACAGGACATTTCTTGAAACTCCTTTGGATGTTGGGATCAAGCTTTTGGAATGGTTTAAGAT TGACAACCTAAGGGATAAGGTGACACGGATTGTACTCTTATGGGTAAATAATCATTTTAATGATTTTGAA GGTGATCCTGCTATGACTCAATTTCTAGAAGAATTTGAAAGAAATTTGGAAGATACAAAAATGAATGGTC ACCTCAGGTTATTGAATATTGCCTGTGCTGCAAAGGCTAAGTGGAGGCAAGTTGTACTGCAGAAGGCGTC CCGTGAGTCCCCGCTGCACTTCTGCCTTACTGGGGGCAGTGAGAAGGGATTTGGAGTCTTTGTTGAAGAA GTAGAATCTGGTAGCAAAGCTGCTGACGCAGGACTGAAAAGGGGTGATCAGGTAATGGAAGTCAATGGAC AAAACTTTGAGAATATTACACTTGCTAAAGCCCTTGAAATTTTGAGAAATAATACTCATCTTGCACTTAC TGTGAAGACCAACATTTTTGTGTTCAAAGAATTGCTCAGTAGGACTGAACAAGAGAAATCTGGTGTTCCT CATATTCCCAAAATTGCTGAAAAAAAAAGTAATCGCCATTCAATTCAAGATGTCCCAGGGGATATGGAGC AGGCACCACAGGAGAAAGGAAATAAGAAAATTAAAGCAAATACCGTTTCAGGTGGAAGAAACAAAATCAG GAAAATTTTGGATAAAACACGATTTAGTATCTTGCCTCCAAAACTATTTAGTGACGGAGGCCTGAGTCAA TCACAAGATGACAGCATTGTGGGAACCAGGCACTGTAGGCACAGCCTGGCTATAATGCCAATTCCCGGAA CACTTTCATCCAGCAGCCCAGATCTCTTACAGCCTACCACTAGCATGTTGGACTTTTCCAATCCTTCAGA TATTCCTGATCAAGTTATAAGAGTTTTCAAAGCAGATCAACAAAGTTGCTACATTATCATCAGTAAGGAT ACCACAGCTAAAGAAGTGGTCTGCCAAGCTGTACAGGAATTCGGTTTGACTGGTGCATCTGACACATACT CCCTCTGTGAAGTGTCTGTTACTCCTGAGGGTGTTATAAAGCAGAGAAGACTTCCGGACCAGTTCTCCAA ATTAGCTGATAGAATTCAACTCAATGGAAGGTATTACCTAAAAAACAACATGGAAACAGAAACATTGTGT TCTGATGAAGATGCTCAGGAACTACTTAAGGAAAGCCAGCTCTCCATGCTGCAGCTCAGTACCATCGAGG TCGCCACCCAGCTCTCCATGAGAGACTTTGATTTATTTCGTAACATTGAGCCTACTGAATACATTGATGA TCTTTTTAAATTAGATTCCAAAACAGGAAATACTCACTTGAAACAATTTGAGGATATTGTAAACCAAGAG ACATTCTGGGTTGCCTCAGAGATTTTAAGTGAGTCAAATCAGCTGAAAAGAATGAAGATTATTAAACATT TTATCAAAATTGCTCTTCATTGCCGAGAATGCAAGAATTTCAATTCCATGTTTGCAATAATAAGTGGCTT GAACCTGGCACCGGTAGCACGACTCAGAGGCACTTGGGAAAAGTTGCCAAGCAAATATGAGAAACATCTT CAAGATCTACAAGACCTTTTTGATCCATCTAGAAATATGGCAAAATACAGAAATATTCTTAGTAGTCAAA GCATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATGACATTTCTCCATGAAGGAAA TGACTCCAAAGTAGATGGCTTAGTAAACTTCGAGAAGCTAAGAATGATTGCCAAGGAAATCCGTCATATT ATTCGAATGACTTCTGCCAACATGGACCCTGCCATGATGTTCCGACAGAGGTCATTGAGTCAAGGCAGCA CAAATTCTAACATGCTAGATGTCCAGGGAGGTGCTCACAAAAAAAGAGCACGCCGCAGCTCACTGCTGAA TGCCAAGAAACTGTATGAAGATGCCCAAATGGCAAGGAAAGTGAAACAGTATCTTTCTAGTCTGGACATA GACACAGATGAGGAGAAGTTCCAGATGATGTCACTGCAGTGGGAACCTGCATATGGTACCTTGACAAAGA ACTTAACTGAAAAAAGGTCAGCCAAATCATCTGAGATGTCTCCTGTACCATTGAGGTCAGTTGGCCAAAC AGCTAAAGTCCACTTGCATCAGCCACACAGAGTGAGCCAGGTGCTTCAGGTGCCAGCAGTTAACTTGCAC CCCATCCGGAAGAAAGGACAAGCCAAAGACCATGTGCTGAGTACAAGTTTACCTCAGAAAGGTTTAGGAC CAACTGAGGAAGTGAGTGTTAAGAAACACACAGAAGACACTATTTCTGTAGCGTCCTCTCTGCACTCTAG CCCTCCTGCATCTCCTCAGAACTCCCCACGCAAAGGTTATACACTCACACCATCAAGTAAATGTGACAAC TTGTCTGACTCCAGCCACAGTGAAATCTCCTCTCGGTCCAGCATTGTGAGCAATGGTTCTGTTGACTCCA TGTCTGCAGCTGGGCAGGATGAACGCTGTTCCTCCCACAGTCTGGCAGTTCCTGAGCCCACAGGGGCATT GGAAAAGACAGATCACCCTTCCGGGATCTCAGACCACAGTCAGCTTGCTCACGGGTGGATGCTGTCAAAG CCGTGTCTTATCAAGGGTGTAGCTGTCTCATCTTCTCTGAGCAGCGAAGAGATGTCTCATGAGCATGTTG TGCTGGAAGCAGCTGACAGTGGGCGGGGCAGTTGGACTTCCTGTTCCAGCAGCTCCCATGACAACTTCCA AAGCCTTCAAAATCAAAAAAGCTGGGACTTCCTGAATTCTTACAGACACATGCATTTAGATGACCCTATT GCAGAAGTTGAGCCCACTGACTGTGAGCCCTGTGCCTGTCCTAAAGGCTGCTCAAGAACTTGTGGCCAGT GCAAAGGCAGCCTAGAGACGAACCAGCTGAGGCAGAGTTGGGCCTCCTCCAGCTCACTCTCGGACACATG TGAACCAAACTACGGGACAGTTAAGCGGAGGGTGTTGGAAAGTGCACCAGCAGAGGCACCTGATGGCTTG GAGCCCAGGGATACCACTGACCCTGTTTATAAAACTGTCACTTCCAGTACAGACAAGGGCTTGATTGTGT ACTGTGTCACCTCACCCAAGAAAGGTGATAGGTATAGGGAGCCACCGCCCACTCCTCCAGGATATCTGGG AATTTCTTTAGCGGACCTAAAGGAAGGACCCCACCCGCACCTAAAACCTCCAGACTATAGTGTGGCAGTA CAGAGATCAAAGATGATGCTTAACAGCCTGTCTAGACTGCCGCCAGCTCCTCCCAGTAGCCACACCTCGG CCTGGGTTCCCTCGAAGATTGGATCCCAGCCTCAAAGGCATAGCCATCCCAAGCTAGCAGATGTGGCTGA TGCAGATAGCGAAGCAGATGAAAACGAGCAGGTGTCAGCAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215350 representing NM_175258
Red=Cloning site Green=Tags(s) MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETI ARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRR RFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQA TESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPA FANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTL DKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKAT PERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFE GDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEE VESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVP HIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQ SQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKD TTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLC SDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQE TFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHL QDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHI IRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDI DTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLH PIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDN LSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSK PCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPI AEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGL EPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAV QRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_175258 |
ORF Size | 4803 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175258.4, NP_780467.2 |
RefSeq Size | 8209 bp |
RefSeq ORF | 4806 bp |
Locus ID | 192786 |
Cytogenetics | 11 B1.3 |
MW | 179.2 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224739 | Rapgef6 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), (10ug) |
USD 1,507.00 |
|
MG215350 | Rapgef6 (tGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), (10ug) |
USD 1,569.00 |
|
MR215350L3 | Lenti ORF clone of Rapgef6 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6) |
USD 1,669.00 |
|
MR215350L4 | Lenti ORF clone of Rapgef6 (mGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6) |
USD 1,669.00 |
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