Rapgef6 (NM_175258) Mouse Tagged ORF Clone

CAT#: MR215350

Rapgef6 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6)

Product Images

Circular map for MR215350

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rapgef6
• Synonyms: A530068K01; AI844632; BB085664; C030018K18Rik; mKIAA4052; PDZ-GEF2; Pdzgef2; RA-GEF-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215350 representing NM_175258
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCGCCCGTGGACCCCGGCGCTCGGCAGGCACTGAGGAAGAAGCCGCCCGAGCGGACTCCTGAGG
  ACTTAAATATTATTTATTCTTATCTGCATGGGATGGAAATACTATCAAACCTCAGGGAGCATCAGCTTAG
  GTTAATGTCTACAAGAGCACGCTATGAGAGATACAGTGGCAATCAAATGCTTTTTTGTTCAGAAACTATT
  GCCAGATGTTGGTATATCCTGCTTTCTGGATCTGTACTTGTAAAAGATTCTATGGTCCTTCCTCCTTGCA
  GTTTTGGTAAGCAGTTTGGAGGAAAAAGAGGATGTGATTGTCTTGTGTTAGAACCTTCAGAAATGATTGT
  GGTAGAGAATTCCAAAGATAATGAAGACAGTATTTTACAAAGAGAAATTCCTGCCAGACAGTCTCGACGA
  AGGTTTCGGAAAATTAACTATAAAGGAGAACGCCAAACCATAATAGATGGAGTGGATATTAATAACTACC
  TTTCGCTTCCAGCTGATCTTACCAAGATGCACCTCACAGACAACCCTCATCCACAGGTGACGCATGTGTC
  TTCTAGTCAGTCTGGCTGTAGCATTGCCAGTGACTCTGGGAGCAGCAGTTTATCCGATATCTATCAGGCT
  ACAGAGAGTGAAGTAGGAGATGTAGATTTAACACGCCTTCCAGAAGGACCTGTTGATTCTGAGGATGAAG
  AGGAGGAGGAAGAAGAGATTGATAGAACAGATCCACTGCAGGGTCGAGATCTTGTTCGGGAGTGTCTTGA
  AAAGGAACCCGCAGACAAAACTGATGATGATGTTGAACAGTTACTTGAGTTCATGCACCAGCTGCCTGCC
  TTTGCGAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCCGTCATGGTTTTTGAAGTAGTAGAGCAGG
  CTGGGGCTGTTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTCATTTTAAATGGCACTGTAGA
  AATTAGTCATCCAGATGGGAAAATAGAAAATCTCTTCATGGGAAATAGTTTTGGGATTGTCCCCACGCTG
  GATAAGCAGCACATGCATGGAGCTGTCAGGACCAAAGTCGATGATTGTCAGTTTGTCTGCATAGCTCAAC
  AAGACTATTGGAGAATTTTAAATCATGTGGAAAAAAATACTCACAAAGTTGAGGAAGAGGGAGAGATTGT
  CATGGTACATGAGCACCGTGAGCTAGACCGGAGCGGAACCAGGAAAGGACACATTGTAATCAAGGCAACA
  CCTGAGCGTCTCATCATGCATTTGATAGAAGAACATTCTATTGTGGACCCAACATACATAGAAGATTTCC
  TCTTAACTTACAGGACATTTCTTGAAACTCCTTTGGATGTTGGGATCAAGCTTTTGGAATGGTTTAAGAT
  TGACAACCTAAGGGATAAGGTGACACGGATTGTACTCTTATGGGTAAATAATCATTTTAATGATTTTGAA
  GGTGATCCTGCTATGACTCAATTTCTAGAAGAATTTGAAAGAAATTTGGAAGATACAAAAATGAATGGTC
  ACCTCAGGTTATTGAATATTGCCTGTGCTGCAAAGGCTAAGTGGAGGCAAGTTGTACTGCAGAAGGCGTC
  CCGTGAGTCCCCGCTGCACTTCTGCCTTACTGGGGGCAGTGAGAAGGGATTTGGAGTCTTTGTTGAAGAA
  GTAGAATCTGGTAGCAAAGCTGCTGACGCAGGACTGAAAAGGGGTGATCAGGTAATGGAAGTCAATGGAC
  AAAACTTTGAGAATATTACACTTGCTAAAGCCCTTGAAATTTTGAGAAATAATACTCATCTTGCACTTAC
  TGTGAAGACCAACATTTTTGTGTTCAAAGAATTGCTCAGTAGGACTGAACAAGAGAAATCTGGTGTTCCT
  CATATTCCCAAAATTGCTGAAAAAAAAAGTAATCGCCATTCAATTCAAGATGTCCCAGGGGATATGGAGC
  AGGCACCACAGGAGAAAGGAAATAAGAAAATTAAAGCAAATACCGTTTCAGGTGGAAGAAACAAAATCAG
  GAAAATTTTGGATAAAACACGATTTAGTATCTTGCCTCCAAAACTATTTAGTGACGGAGGCCTGAGTCAA
  TCACAAGATGACAGCATTGTGGGAACCAGGCACTGTAGGCACAGCCTGGCTATAATGCCAATTCCCGGAA
  CACTTTCATCCAGCAGCCCAGATCTCTTACAGCCTACCACTAGCATGTTGGACTTTTCCAATCCTTCAGA
  TATTCCTGATCAAGTTATAAGAGTTTTCAAAGCAGATCAACAAAGTTGCTACATTATCATCAGTAAGGAT
  ACCACAGCTAAAGAAGTGGTCTGCCAAGCTGTACAGGAATTCGGTTTGACTGGTGCATCTGACACATACT
  CCCTCTGTGAAGTGTCTGTTACTCCTGAGGGTGTTATAAAGCAGAGAAGACTTCCGGACCAGTTCTCCAA
  ATTAGCTGATAGAATTCAACTCAATGGAAGGTATTACCTAAAAAACAACATGGAAACAGAAACATTGTGT
  TCTGATGAAGATGCTCAGGAACTACTTAAGGAAAGCCAGCTCTCCATGCTGCAGCTCAGTACCATCGAGG
  TCGCCACCCAGCTCTCCATGAGAGACTTTGATTTATTTCGTAACATTGAGCCTACTGAATACATTGATGA
  TCTTTTTAAATTAGATTCCAAAACAGGAAATACTCACTTGAAACAATTTGAGGATATTGTAAACCAAGAG
  ACATTCTGGGTTGCCTCAGAGATTTTAAGTGAGTCAAATCAGCTGAAAAGAATGAAGATTATTAAACATT
  TTATCAAAATTGCTCTTCATTGCCGAGAATGCAAGAATTTCAATTCCATGTTTGCAATAATAAGTGGCTT
  GAACCTGGCACCGGTAGCACGACTCAGAGGCACTTGGGAAAAGTTGCCAAGCAAATATGAGAAACATCTT
  CAAGATCTACAAGACCTTTTTGATCCATCTAGAAATATGGCAAAATACAGAAATATTCTTAGTAGTCAAA
  GCATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATGACATTTCTCCATGAAGGAAA
  TGACTCCAAAGTAGATGGCTTAGTAAACTTCGAGAAGCTAAGAATGATTGCCAAGGAAATCCGTCATATT
  ATTCGAATGACTTCTGCCAACATGGACCCTGCCATGATGTTCCGACAGAGGTCATTGAGTCAAGGCAGCA
  CAAATTCTAACATGCTAGATGTCCAGGGAGGTGCTCACAAAAAAAGAGCACGCCGCAGCTCACTGCTGAA
  TGCCAAGAAACTGTATGAAGATGCCCAAATGGCAAGGAAAGTGAAACAGTATCTTTCTAGTCTGGACATA
  GACACAGATGAGGAGAAGTTCCAGATGATGTCACTGCAGTGGGAACCTGCATATGGTACCTTGACAAAGA
  ACTTAACTGAAAAAAGGTCAGCCAAATCATCTGAGATGTCTCCTGTACCATTGAGGTCAGTTGGCCAAAC
  AGCTAAAGTCCACTTGCATCAGCCACACAGAGTGAGCCAGGTGCTTCAGGTGCCAGCAGTTAACTTGCAC
  CCCATCCGGAAGAAAGGACAAGCCAAAGACCATGTGCTGAGTACAAGTTTACCTCAGAAAGGTTTAGGAC
  CAACTGAGGAAGTGAGTGTTAAGAAACACACAGAAGACACTATTTCTGTAGCGTCCTCTCTGCACTCTAG
  CCCTCCTGCATCTCCTCAGAACTCCCCACGCAAAGGTTATACACTCACACCATCAAGTAAATGTGACAAC
  TTGTCTGACTCCAGCCACAGTGAAATCTCCTCTCGGTCCAGCATTGTGAGCAATGGTTCTGTTGACTCCA
  TGTCTGCAGCTGGGCAGGATGAACGCTGTTCCTCCCACAGTCTGGCAGTTCCTGAGCCCACAGGGGCATT
  GGAAAAGACAGATCACCCTTCCGGGATCTCAGACCACAGTCAGCTTGCTCACGGGTGGATGCTGTCAAAG
  CCGTGTCTTATCAAGGGTGTAGCTGTCTCATCTTCTCTGAGCAGCGAAGAGATGTCTCATGAGCATGTTG
  TGCTGGAAGCAGCTGACAGTGGGCGGGGCAGTTGGACTTCCTGTTCCAGCAGCTCCCATGACAACTTCCA
  AAGCCTTCAAAATCAAAAAAGCTGGGACTTCCTGAATTCTTACAGACACATGCATTTAGATGACCCTATT
  GCAGAAGTTGAGCCCACTGACTGTGAGCCCTGTGCCTGTCCTAAAGGCTGCTCAAGAACTTGTGGCCAGT
  GCAAAGGCAGCCTAGAGACGAACCAGCTGAGGCAGAGTTGGGCCTCCTCCAGCTCACTCTCGGACACATG
  TGAACCAAACTACGGGACAGTTAAGCGGAGGGTGTTGGAAAGTGCACCAGCAGAGGCACCTGATGGCTTG
  GAGCCCAGGGATACCACTGACCCTGTTTATAAAACTGTCACTTCCAGTACAGACAAGGGCTTGATTGTGT
  ACTGTGTCACCTCACCCAAGAAAGGTGATAGGTATAGGGAGCCACCGCCCACTCCTCCAGGATATCTGGG
  AATTTCTTTAGCGGACCTAAAGGAAGGACCCCACCCGCACCTAAAACCTCCAGACTATAGTGTGGCAGTA
  CAGAGATCAAAGATGATGCTTAACAGCCTGTCTAGACTGCCGCCAGCTCCTCCCAGTAGCCACACCTCGG
  CCTGGGTTCCCTCGAAGATTGGATCCCAGCCTCAAAGGCATAGCCATCCCAAGCTAGCAGATGTGGCTGA
  TGCAGATAGCGAAGCAGATGAAAACGAGCAGGTGTCAGCAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215350 representing NM_175258
  Red=Cloning site Green=Tags(s)
  
  MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETI
  ARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRR
  RFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQA
  TESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPA
  FANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTL
  DKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKAT
  PERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFE
  GDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEE
  VESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVP
  HIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQ
  SQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKD
  TTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLC
  SDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQE
  TFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHL
  QDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHI
  IRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDI
  DTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLH
  PIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDN
  LSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSK
  PCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPI
  AEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGL
  EPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAV
  QRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175258
• ORF Size: 4803 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175258.4, NP_780467.2
• RefSeq Size: 8209 bp
• RefSeq ORF: 4806 bp
• Locus ID: 192786
• Cytogenetics: 11 B1.3
• MW: 179.2 kDa