Mcidas (NM_001037914) Mouse Tagged ORF Clone

CAT#: MR214707

Mcin (Myc-DDK-tagged) - Mouse predicted gene 6320 (Gm6320)

Product Images

Circular map for MR214707

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mcidas
• Synonyms: EG622408; Gm6320; Idas; Mci; Mcin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR214707 representing NM_001037914
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGCGTGCGAGGGCAGCGCAGCCGGACGCCGGGCCTTCGACAGCATCTGCCCCAACAGGATGCTGG
  ACCTGTCGCGGCGGACCCTCGGCAAGCCCGGGAAGCCGGAGAGGAAGTTCGTTCCTTCGTGGAAGTCCTT
  TTCGGGATGCGGTGGCGGCAGCCCGGTGGCGGTGTACGAGGACCCTCCGGACGCAGAGCCCGCCCCACTG
  CCAGCTCTCACAACCATAGACCTGCAGGACCTCGCCGACTGCACCTCGCTGCTCGGAACCGAAGCGTCTC
  CTAGTGGTGATTCGTCCGCGTCGCAGAACCCCTCCTTGCAAACTGAAGAAGACTTCAACCTGCAGAATTT
  CAGAGATGCAATGGATGACCTCATTGCAGATTCATCCTCTTTGATGTCGCCTCCCCTGACCAACAGTGAC
  TTTCCCTTTTCCCCTTGTGATGTTTCGTCTTTCGGGTCCTGCCTCTCCCCATCGCTGGACCCACCTGCCT
  TGGGGTCTCCAGACCTGCCGCCACCACCAACGGAGCAGTACTGGAAGGAGGTGGCTGACCAGAACCAGAG
  GGCACTGGGGACTGCTCTCATAGAGAATAACCAACTGCACGTGACGCTGACGCAGAAGCAGGAGGAGATA
  GCCTCGCTCCGGGAGAGGAACGTGCAGCTGAAAGAACTCGCCAGCCGGACCCGGCACCTGGCCTCAGTGC
  TGGATAAGCTGATGATCACGCAGTCTCCTGCCGAGCCCTTCCAGATCAAGGCAACAACGAAAAGGAGCCT
  GGAGGAGCTGTTCTGTGCTGCGGGGCAAGCAGGGCAGGGTTGCGCGGAAGTGGACGCCATCCTCAGAGAC
  ATCTCCCAGCGCTGCGAGGAAGCCCTGCACAATCGTGACCCTAAGCGGCCCAGGCTGCAGCCAGAGCCAG
  ACAGCAAGGACTGCAGCTCCAGGAACCTCCACGGCGCCTTCCGAGGACTGCGCACCGACTGCAGCGCCAG
  CTCGGTGAATCTGAGTCACAGTGAGCTGGAGGAAGGCGGCTCCTTCAGCACGCCCATCCGCAGCCACAGT
  ACCATCCGCACCCTGGCTTTCCCCCAGGGCAAAGCCTTCACCATCCGGACAGTCACCGGTGGTTACAAAT
  TCCGCTGGGTCCCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR214707 representing NM_001037914
  Red=Cloning site Green=Tags(s)
  
  MQACEGSAAGRRAFDSICPNRMLDLSRRTLGKPGKPERKFVPSWKSFSGCGGGSPVAVYEDPPDAEPAPL
  PALTTIDLQDLADCTSLLGTEASPSGDSSASQNPSLQTEEDFNLQNFRDAMDDLIADSSSLMSPPLTNSD
  FPFSPCDVSSFGSCLSPSLDPPALGSPDLPPPPTEQYWKEVADQNQRALGTALIENNQLHVTLTQKQEEI
  ASLRERNVQLKELASRTRHLASVLDKLMITQSPAEPFQIKATTKRSLEELFCAAGQAGQGCAEVDAILRD
  ISQRCEEALHNRDPKRPRLQPEPDSKDCSSRNLHGAFRGLRTDCSASSVNLSHSELEEGGSFSTPIRSHS
  TIRTLAFPQGKAFTIRTVTGGYKFRWVPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037914
• ORF Size: 1137 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037914.3, NP_001033003.1
• RefSeq Size: 2354 bp
• RefSeq ORF: 1140 bp
• Locus ID: 622408
• UniProt ID: Q3UZ45
• Cytogenetics: 13
• MW: 41.6 kDa
• Gene Summary: Transcription regulator specifically required for multiciliate cell differentiation. Acts in a multiprotein complex containing E2F4 and E2F5 that binds and activates genes required for centriole biogenesis. Required for the deuterosome-mediated acentriolar pathway. Plays a role in mitotic cell cycle progression by promoting cell cycle exit. Modulates GMNN activity by reducing its affinity for CDT1.[UniProtKB/Swiss-Prot Function]