Ankrd34a (NM_001024851) Mouse Tagged ORF Clone

CAT#: MR214346

Ankrd34a (Myc-DDK-tagged) - Mouse ankyrin repeat domain 34A (Ankrd34a)

Product Images

Circular map for MR214346

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankrd34a
• Synonyms: Ankrd34; AW060394
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR214346 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCACACCGAGGGCCACGCTCTTCTTCGAGCTGTGGGACAGGGTAAGCTACGCTTGGCCCGTTTGC
  TTCTGGAGGGAGGAGCCTACGTGAATGAGGGTGATGCCCAGGGGGAGACTGCGCTAATGGCGGCCTGTAG
  GGCCCGTTACGACGACCCTCAGAACAAGGCGCGCATGGTACGCTATCTCTTGGAGCAAGGCGCGGATCCC
  AACATCGCAGACCGTTTAGGACGTACCGCGCTGATGCATGCTTGCGCCGGGGGTGGGGGAGCCGCAGTGG
  CCTCTCTGCTCCTTGCCCACGGCGCAGACCCTTCAGTTCGAGATCACGCTGGCGCCTCGGCGCTTGTCCA
  CGCCCTGGACCGAGGGGACCGCGAGACCCTTGCCACACTGCTGGACGCCTGCAAGGCCAAGGGCACGGAG
  GTCATCATCATAACGACGGATACCTCGCCCTCGGGCACCAAGAAGACCAGGCAGTATCTTAATTCCCCAC
  CGTCCCCAGGGGTAGAGGACCCTGCACCCGCTCCTCCTAGTCCAGGGGTCTGCACATCGCCTTCAGAAGT
  CCAACTGCAGACTGCAGGAGGAGGGCGCGGGTTGTTATCCCCTCGCGCCCAGGAAGAAGAGGAGAAAAGA
  GACATTTTTGAATTTCCTCTTCCCAAGCCCCCTGATGACCCTTCCCCTTCCGAGCCTCTCCCCAAGCCAC
  CTCGACATCCTCCAAAACCACTCAAAAGGCTCAATTCCGAGCCCTGGGGCCTTGTGGCTCCTCCTCAACC
  GGTTCCTCCTGCAGAAGGGAGGCCCGGTTTGGAGCGTCTGGCTGCTGAATTCAATGGCTTGACCCTGACC
  GGGAGACCGCGTCTTTCTCGGCGTCACAGCACCGAAGGCCCGGAGGACCCGCCCCCATGGGCGGAGAAAG
  TGACGGGTGGGGGTCCTCTCTCGCGCCGAAACACTGCGCCCGAGGCTCAGGAGTCTGGTCTTCCTTCAGG
  GCTAAGGCAAAAACTGAGCCGCATGGAGCCGGTGGAGCTCGACACCCCTGGACATTTTTGCCCTGACTCC
  CCCGAGTCCAGCCGGTTATCCCTGGAGCGCCGGCGATACAGCGCCTCTCCGCTGACCCTCCCTCCAGCGG
  GCTCAGTTTCCTCCCCACGCCAGTCCCAGGAAAGTCTGCCCGGGGCTGTATCCCCGCTGAGCGGACGGAG
  GCGGAGTCCAGGGCTGCTAGAGAGAAGGGGCTCCGGGACCTTGCTCCTGGACCACATCTCGCAAACACGA
  CCAGGTTTCTTGCCTCCTCTCAACGTCAGTCCCCACCCTCCCATCCCCGACATTCGTCCCCAACCCGGAG
  GTCGGGCACCCTCACTGCCTGCCCCTCCACCCTCTGGGGCACCAGGGTCTCCCAGGACCAAGCGCAAGTT
  GGTGAGACGCCACTCCATGCAGACTGAGCAGATTCGCCTGCTAGGGGGGTTTCAGAGTCTAGGTGGGCCT
  GGGGAACCCGGGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR214346 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLHTEGHALLRAVGQGKLRLARLLLEGGAYVNEGDAQGETALMAACRARYDDPQNKARMVRYLLEQGADP
  NIADRLGRTALMHACAGGGGAAVASLLLAHGADPSVRDHAGASALVHALDRGDRETLATLLDACKAKGTE
  VIIITTDTSPSGTKKTRQYLNSPPSPGVEDPAPAPPSPGVCTSPSEVQLQTAGGGRGLLSPRAQEEEEKR
  DIFEFPLPKPPDDPSPSEPLPKPPRHPPKPLKRLNSEPWGLVAPPQPVPPAEGRPGLERLAAEFNGLTLT
  GRPRLSRRHSTEGPEDPPPWAEKVTGGGPLSRRNTAPEAQESGLPSGLRQKLSRMEPVELDTPGHFCPDS
  PESSRLSLERRRYSASPLTLPPAGSVSSPRQSQESLPGAVSPLSGRRRSPGLLERRGSGTLLLDHISQTR
  PGFLPPLNVSPHPPIPDIRPQPGGRAPSLPAPPPSGAPGSPRTKRKLVRRHSMQTEQIRLLGGFQSLGGP
  GEPGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024851
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024851.3, NP_001020022.2
• RefSeq Size: 2586 bp
• RefSeq ORF: 1488 bp
• Locus ID: 545554
• Cytogenetics: 3 F2.1
• MW: 52.5 kDa