Elmsan1 (NM_001163502) Mouse Tagged ORF Clone

CAT#: MR212835

Elmsan1 (Myc-DDK-tagged) - Mouse RIKEN cDNA C130039O16 gene (C130039O16Rik), transcript variant 1

Product Images

Circular map for MR212835

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Elmsan1
• Synonyms: 9430029N19Rik; C130039O16Rik; Gm260
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212835 representing NM_001163502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCCAGGCCCAGTCCAAGGCTCAGAGTAAACGCAAGCGTTGTCCTTTTGGGGACCAGGAGCCAG
  CCGCCAAGGAACAGCCACCACCACTGCAGAGTCCACCACAGTCCCTCAGAGCTAAGGAGGAACAGTACGG
  GGCCCACGAAGGTCCCACAGGGGTGGCCTCCACCACCCAGCCTGTGGAGCTGTCTCCTCCTAACAACCTG
  GCTCTCCTGAACAGTGTGGTGTATGGATCTGAGAGGACCACGGCAGCCATGTTGTCCCAGCAGGCCCAAG
  TAAGCTCGGTAAAGTGGCCCAGCTCTGTGATGGCTCCAGGGCGGGGCCTGGAACGTGGCGGAGGTGGAGG
  CATCAGTGACAGTGGCTGGCAGCAGCAACCTGGCCAGCCCCCACCCCACTCCACGTGGAATCACCTGCCC
  CTCTACGGTGGACCCAAAGGGAGCCCTCATCCAGGTGTGGGGGTACCTCCCTACTATAACCACCCGGAGG
  CACTGAAGGGGAACAAACCTGGAGGTCCCCAGCTGGATCACTATGGAAACGCAGTGCAGCCCATGGTGCC
  ACAGAAGGTGCAGCTGGAGGTGGGGAGGCCCCAAGCACCCTTGAACTCTTTCCATGCAGCCAAGAAACCC
  CCAAACCAGACACTGCCCTTGCAACCCTTCCAGCTGGCATTTGGCCACCAGGTGAACCGTCAGGTCTTCC
  GGCAGGGCCCTCAGCCCTCTAACCCCACCGCCTCCTTCCCACCTCAGAAGCAGCAGCAGCAACAGCAACC
  GGCAGCCCTGCCCCAGATGCAGCTATTTGAGAACTACTACCCCATGCATCAACTGCCTTCGCAACAGCAC
  CAGGACTTTGGCCTGGCACCAGGTGGGCCACTGGGACAGACCCACCTGGCTCACCGCAGCATGGCCCCTT
  ACCCCTTTTCCCACAACCCAGATATGAACCCAGAACTGCGCAAGGCTCTCCTGCAGGACCCTGCTTCACA
  GCCCGTGCTACCTCAGCCCCAGATGGCCTTCCCACGTCGCTCCCGCCGTCTCTCCAAGGAGGGAATTCTG
  CCTTCCAACTCCCTCGATGGAGCTGGCACCCAACCTGGGCAGGAGCCCGCGAGCAATCTGTTCCTTCACC
  ACTGGTCTCTGCCGCAGCCACCGCCAGGCACCCTGGGGCAGCCCCATTCTGAAGCCCTGGGATTCCCGTT
  AGAACTGAGGGAGTCACAGATGCTGGCGGATGGGGACAGACTGGCACCCAATGGTCGGGAGCGGGAGCCT
  CCCGCCATGGGTAATGAGGAGGTCATGAGAGCAGGGGGCCTCGGGGACTGTGGACAGATGATACGAAGTG
  GGGTGATCCAGAGCACAAAACGGAGACGCAGGGTGTCCCAGGAGGCTAATTTGCTGACCCTGGCCCAGAA
  GGCAGTGGAGCTGGCCTCTATGCAGGATGCCAATGGCTCTGAGGAGAAGCGGAAAAGCGTGTTGGCCACA
  ACTTCCAGGTGTGGCGTGGAGTTTTCCGAGCCTGCCTTAGCCGCCAAGAGAGCTCGGGAGGAGAGTGGGA
  TGGTACCCCTCATCATTCCTGTGTCTGTTCCCGTGAGGACTGTGGGTCCAACTGAGGTGGCCCAAGTCGG
  AGGTGCTGACGAGGATGGGACTGGTCTTGAGCAGTACCCCACCGAGCACAAGCCGTCAGTCATCGTGACC
  CGCAGGCGGTCCACCCGAGTTCCCGGGACAGATGCTGCAGCTCAGGCTGAAGACCTGAACGTCAAGTTGG
  AAGGGGAGCCTTCCATGCGGAAACCAAAGCAGCGGCCGCGGCCGGAGCCCCTCATCATCCCCACCAAGGC
  GGGCACTTTCATCGCCCCTCCTGTCTACTCCAACATCACCCCTTACCAGAGCCACCTGCGCTCTCCCGTG
  CGCCTTGCTGACCACCCCTCTGAGCGGAGCTTTGAGCCCCCCCCTTACACACCACCCCCCATTCTCAGCC
  CCGTCCGGGAAGGCTCTGGCCTCTACTTCAATGCCATCATATCAACCAGCAACATCCCGGCCCCTCCTCC
  TATCACGCCAAAGAGTGCCCATCGAACCCTGCTCCGCTCTAATAGCTCTGAAGTCACCCCGCCTGTCCTC
  TCTGTGATGGGGGAGGCCACCCCTGTGAGCATCGAACCACGAATCAACGTCGGGACCCGGTTCCAGGCAG
  AAATCCCCATGATGAGAGACCGAGCGCTGGCAGCTTTTGACCCCCATAAGGCTGACTTGGTGTGGCAGCC
  ATGGGAACATCTTGAGAGCAGCTGGGAGAAGCAGAGACAAGTGGATGACCTGCTGACAGCTGCCTGTTCA
  AGCATTTTCCCTGGGGCCGGCACCAACCAGGAGCTGGCCCTGCACTATCTGCATGAGTCCCGGGGGGACA
  TCCTGGAGGCGCTGAATAAGCTGCTACTGAGGAAGCCCCTGCGGCCTCACAATCACCCACTGGCGACATA
  TCACTACACAGGCTCTGACCAGTGGAAGACGGCTGAGAGGAAGCTGTTCAACAAGGGCATTGCCATCTAT
  AAGAAAGATTTCTTCCTGGTGCAGAAGCTGATCCAGACCAAGACTGTGGCCCAGTGCGTGGAATTCTACT
  ACACCTACAAGAAACAGGTGAAAATCGGCCGCAACGGGACGCTCACCTTCGGCGACCTGGATATCGGTGA
  CGAGAAGTCAGGCCAAGAGGAGGTTGAGGTGGACGTTAAGACTTCTCAGAAGTTCCCAAGGGTGCCTCCT
  CCCAGAAGAGAGTCCCCAAGTGAAGAGAGGCTGGAGCCTAAGAGAGAAGTGACAGAACCCAGGAAGGAGG
  GGGAGGAGGAGGTGCCAGACACCCAGGAGAAGGGAGAGCAGGAGGAGGGGCGTGAGCGCTGCCGGCGGGC
  CGCTGCCGTCAAAGCTACACAGACACTACAGGCCAATGAGGCGGCCAATGACGTTCTCATTCTCCGGAGC
  CATGAGCCCAACGCCCCGGGGTCTGCAGGTATCCAGACCTCAGAGAAGCCGAGGGAGGGGCCGGGGAAGT
  CCCGAAGGGCACTGCCTTTCACAGAGAAGAAGAAAAAAGCCGAGGCATTTAATAAAACCCAGAACCAGGA
  GAACACCTTCCCTTGTAAAAAGTGCGGCAGGGTGTTTTACAAGGTGAAGAGCCGCAGTGCTCACATGAAG
  AGTCACGCAGAGCAAGAGAAGAAGGCCGCAGCTCTGAGGCTGAAGGAGAAGGAGGCTGCAGCGGCCGCCG
  CACATCAGCAGGCGCTGAGAGAAGAGAGCGGCGAAGGGGAGAAGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212835 representing NM_001163502
  Red=Cloning site Green=Tags(s)
  
  MNLQAQSKAQSKRKRCPFGDQEPAAKEQPPPLQSPPQSLRAKEEQYGAHEGPTGVASTTQPVELSPPNNL
  ALLNSVVYGSERTTAAMLSQQAQVSSVKWPSSVMAPGRGLERGGGGGISDSGWQQQPGQPPPHSTWNHLP
  LYGGPKGSPHPGVGVPPYYNHPEALKGNKPGGPQLDHYGNAVQPMVPQKVQLEVGRPQAPLNSFHAAKKP
  PNQTLPLQPFQLAFGHQVNRQVFRQGPQPSNPTASFPPQKQQQQQQPAALPQMQLFENYYPMHQLPSQQH
  QDFGLAPGGPLGQTHLAHRSMAPYPFSHNPDMNPELRKALLQDPASQPVLPQPQMAFPRRSRRLSKEGIL
  PSNSLDGAGTQPGQEPASNLFLHHWSLPQPPPGTLGQPHSEALGFPLELRESQMLADGDRLAPNGREREP
  PAMGNEEVMRAGGLGDCGQMIRSGVIQSTKRRRRVSQEANLLTLAQKAVELASMQDANGSEEKRKSVLAT
  TSRCGVEFSEPALAAKRAREESGMVPLIIPVSVPVRTVGPTEVAQVGGADEDGTGLEQYPTEHKPSVIVT
  RRRSTRVPGTDAAAQAEDLNVKLEGEPSMRKPKQRPRPEPLIIPTKAGTFIAPPVYSNITPYQSHLRSPV
  RLADHPSERSFEPPPYTPPPILSPVREGSGLYFNAIISTSNIPAPPPITPKSAHRTLLRSNSSEVTPPVL
  SVMGEATPVSIEPRINVGTRFQAEIPMMRDRALAAFDPHKADLVWQPWEHLESSWEKQRQVDDLLTAACS
  SIFPGAGTNQELALHYLHESRGDILEALNKLLLRKPLRPHNHPLATYHYTGSDQWKTAERKLFNKGIAIY
  KKDFFLVQKLIQTKTVAQCVEFYYTYKKQVKIGRNGTLTFGDLDIGDEKSGQEEVEVDVKTSQKFPRVPP
  PRRESPSEERLEPKREVTEPRKEGEEEVPDTQEKGEQEEGRERCRRAAAVKATQTLQANEAANDVLILRS
  HEPNAPGSAGIQTSEKPREGPGKSRRALPFTEKKKKAEAFNKTQNQENTFPCKKCGRVFYKVKSRSAHMK
  SHAEQEKKAAALRLKEKEAAAAAAHQQALREESGEGEKG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163502
• ORF Size: 3267 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163502.1, NP_001156974.1
• RefSeq Size: 7266 bp
• RefSeq ORF: 3270 bp
• Locus ID: 238317
• Cytogenetics: 12 D1
• MW: 119.7 kDa