Ltbp1 (NM_206958) Mouse Tagged ORF Clone

CAT#: MR211964

Ltbp1 (Myc-DDK-tagged) - Mouse latent transforming growth factor beta binding protein 1 (Ltbp1), transcript variant 2

Product Images

Circular map for MR211964

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ltbp1
• Synonyms: 9430031G15Rik; 9830146M04; b2b1000Clo; Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211964 representing NM_206958
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACTAAGCTGATGTGTTTGTTGTTCTTTCTCTGTCTGCCTCTACTCCTAGTGAGTAACCACACTG
  GTCGCATCAAGGTGGTCTTTACTCCGAGCATCTGTAAAGTGACCTGCACCAAGGGCAACTGTCAGAACAG
  CTGCCAGAAGGGGAATACCACCACTCTCATTAGTGAGAACGGCCATGCAGCCGACACCCTGACAGCCACG
  AACTTCCGAGTGGTTATTTGCCATCTTCCGTGTATGAATGGTGGCCAGTGCAGTTCAAGGGACAAATGTC
  AGTGCCCTCCAAATTTCACAGGAAAGCTTTGCCAGATCCCTGTCCTTGGTGCCAGTATGCCCAAACTCTA
  CCAGCACGCCCAGCAGCAGGGCAAGGCATTGGGGAGTCACGTCATCCATTCCACACATACCTTGCCTCTA
  ACCATGACTAGCCAGCAAGGGGTCAAAGTGAAATTCCCCCCCAACATAGTCAATATCCATGTGAAACATC
  CTCCCGAGGCTTCTGTCCAGATACACCAGGTTTCCAGGATTGACAGCCCAGGAGGCCAGAAGGTGAAGGA
  AGCCCAACCAGGCCAGTCCCAAGTCTCTTACCAAGGACTTCCGGTCCAGAAGACCCAGACAGTCCATTCT
  ACGTACTCCCACCAGCAGCTTATTCCCCATGTGTATCCCGTGGCTGCTAAGACACAACTTGGCCGATGCT
  TCCAGGAAACCATTGGATCACAGTGTGGCAAAGCGCTCCCTGGCCTTTCCAAGCAAGAGGACTGCTGTGG
  GACAGTGGGGACGTCCTGGGGCTTTAACAAATGCCAGAAGTGCCCCAAGAAACAATCCTATCATGGATAT
  ACTCAAATGATGGAATGCCTACAAGGATATAAACGGGTTAACAACACCTTTTGTCAAGATATTAATGAAT
  GTCAGCTACAAGGTGTATGCCCTAATGGTGAGTGTTTGAATACCATGGGCAGCTACAGATGCTCCTGCAA
  AATGGGATTCGGGCCTGACCCTACCTTTTCAAGTTGTGTTCCGGATCCCCCTGTGATCTCAGAAGAGAAA
  GGGCCCTGTTACCGCCTTGTCAGTCCTGGAAGGCATTGTATGCACCCTCTCTCTGTTCATCTCACCAAGC
  AGATCTGCTGTTGTAGTGTGGGCAAGGCCTGGGGCCCACATTGTGAGAAATGTCCCCTCCCAGGCACAGC
  TGCTTTTAAGGAAATCTGTCCTGGTGGAATGGGTTATACGGTTTCTGGCGTTCATAGACGCAGGCCAATC
  CATCAGCATATAGGTAAAGAAGCTGTATATGTCAAGCCAAAGAACACTCAACCTGTTGCTAAAAGTACTC
  ATCCTCCACCTCTCCCAGCCAAGGAAGAGCCTGTGGAGGCCCTGACCTCCTCGTGGGAACACGGGCCGAG
  AGGGGCGGAGCCCGAAGTGGTAACAGCACCTCCTGAGAAGGAAATACCTTCACTGGATCAAGAGAAAACC
  AGACTTGAACCTGGGCAACCCCAGCTCTCCCCAGGGGTTTCAACTATTCATCTGCACCCACAGTTTCCAG
  TAGTGGTTGAAAAGACATCCCCTCCTGTGCCTGTGGAAGTAGCTCCTGAAGCTTCCACATCCAGTGCCAG
  CCAAGTGATTGCTCCTACGCAAGTAACAGAAATCAACGAATGTACTGTGAACCCGGACATTTGCGGAGCA
  GGACACTGCATCAACCTGCCGGTGCGGTATACCTGCATATGCTACGAGGGCTACAAGTTCAGTGAACAGC
  TGAGGAAATGTGTCGACATTGATGAATGTGCTCAGGTCCGGCACCTCTGCTCCCAGGGTCGCTGTGAGAA
  CACAGAGGGAAGTTTCCTGTGTGTCTGCCCGGCAGGGTTTATGGCCAGTGAGGAGGGTACTAACTGTATA
  GATGTGGATGAGTGCCTGAGGCCTGATATGTGTAGGGATGGCCGCTGCATCAACACCGCTGGGGCCTTCC
  GGTGCGAGTACTGTGACAGTGGGTACCGGATGTCACGGAGAGGCTACTGTGAGGATATTGATGAGTGTCT
  GAAGCCAAGCACTTGTCCCGAGGAACAGTGTGTGAACACCCCGGGCTCTTACCAGTGTGTGCCCTGCACA
  GAGGGGTTCCGCGGCTGGAATGGACAATGCCTTGATGTGGACGAGTGCCTGCAGCCAAAGGTCTGTACGA
  ATGGTTCCTGCACCAACCTGGAAGGGTCCTACATGTGTTCCTGCCACAGGGGCTACAGCCCCACACCAGA
  CCATAGACACTGTCAAGATATTGATGAATGCCAACAAGGGAATCTCTGCATGAATGGGCAGTGCAGAAAT
  ACCGATGGCTCCTTCCGGTGTACCTGTGGACAGGGCTATCAGCTGTCAGCAGCTAAAGACCAATGTGAAG
  ATATTGATGAATGCGAGCACCATCACCTCTGCTCTCACGGGCAGTGCAGGAACACAGAGGGCTCCTTCCA
  GTGTGTGTGCAACCAGGGTTACAGAGCATCTGTACTTGGAGACCACTGTGAGGATATCAATGAATGCTTG
  GAGGACAGCAGTGTCTGCCAGGGAGGTGACTGCATCAATACTGCGGGGTCCTATGATTGCACATGCCCGG
  ATGGATTCCAGCTGAACGACAATAAGGGCTGTCAAGACATTAATGAATGTGCACAGCCAGGACTCTGTGG
  ATCTCATGGGGAGTGTCTAAACACACAAGGCTCCTTCCACTGTGTCTGTGAACAGGGGTTCTCCATCTCT
  GCCGATGGCCGTACATGCGAAGATATTGATGAGTGTGTTAACAACACTGTGTGTGACAGTCACGGCTTCT
  GTGACAACACAGCCGGCTCTTTCCGCTGCCTCTGTTATCAGGGCTTTCAAGCCCCACAGGATGGGCAAGG
  GTGTGTGGATGTGAACGAGTGTGAACTGCTCAGTGGTGTATGTGGGGAAGCCTTCTGTGAAAACGTGGAA
  GGATCCTTCCTGTGTGTGTGTGCCGATGAGAACCAGGAGTACAGCCCCATGACTGGGCAGTGTCGCTCTC
  GGGTCACGGAAGATTCAGGTGTGGATCGTCAGCCTAGAGAAGAGAAGAAGGAGTGCTATTATAACCTCAA
  TGATGCTAGTCTCTGTGATAATGTCCTGGCCCCCAACGTCACCAAACAAGAGTGCTGCTGTACGTCAGGT
  GCCGGCTGGGGAGACAACTGTGAGATCTTCCCTTGCCCCGTCCAGGGAACTGCTGAGTTCACGGAAATGT
  GCCCTAGAGGGAAAGGTTTGGTCCCGGCTGGAGAATCCTCTTACGACACTGGTGGCGAGAACTACAAAGA
  TGCTGATGAATGCTTGCTGTTTGGAGAGGAAATCTGTAAAAATGGTTACTGTTTGAACACTCAACCTGGG
  TATGAATGCTACTGTAAGCAAGGGACATACTATGATCCTGTCAAATTACAGTGCTTTGATATGGATGAAT
  GCCAAGACCCTAACAGTTGCATTGACGGCCAGTGTGTGAACACAGAGGGCTCCTACAACTGCTTTTGCAC
  CCACCCAATGGTCCTGGATGCCTCTGAGAAGAGATGTGTGCAACCAACTGAATCAAATGAACAAATAGAA
  GAGACCGATGTCTATCAAGATCTGTGCTGGGAGCATCTGAGTGAGGAGTACGTGTGTAGCCGTCCTCTCG
  TAGGCAAGCAGACGACGTACACAGAATGCTGCTGTTTGTACGGGGAGGCTTGGGGCATGCAGTGTGCTCT
  GTGCCCCATGAAGGACTCAGATGACTATGCCCAGCTGTGCAATATCCCTGTGACTGGGCGTCGGCGGCCA
  TATGGACGGGATGCATTGGTGGACTTCAGTGAACAGTATGGCCCAGAAACAGACCCTTACTTCATCCAGG
  ATCGCTTTCTAAATAGCTTTGAGGAGCTACAGGCTGAGGAGTGTGGCATCCTCAATGGTTGTGAAAACGG
  CCGCTGTGTGAGAGTCCAGGAAGGATACACTTGCGATTGCTTTGATGGATATCACCTGGATATGGCCAAG
  ATGACCTGTGTCGATGTAAATGAATGCAGCGAGCTGAATAATCGGATGTCTCTCTGCAAGAACGCCAAGT
  GCATTAACACAGAAGGCTCCTACAAATGCTTGTGTCTGCCAGGCTACATACCATCCGACAAGCCCAACTA
  CTGTACACCCCTGAACAGCGCTTTGAATTTAGACAAAGAAAGTGACCTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211964 representing NM_206958
  Red=Cloning site Green=Tags(s)
  
  MDTKLMCLLFFLCLPLLLVSNHTGRIKVVFTPSICKVTCTKGNCQNSCQKGNTTTLISENGHAADTLTAT
  NFRVVICHLPCMNGGQCSSRDKCQCPPNFTGKLCQIPVLGASMPKLYQHAQQQGKALGSHVIHSTHTLPL
  TMTSQQGVKVKFPPNIVNIHVKHPPEASVQIHQVSRIDSPGGQKVKEAQPGQSQVSYQGLPVQKTQTVHS
  TYSHQQLIPHVYPVAAKTQLGRCFQETIGSQCGKALPGLSKQEDCCGTVGTSWGFNKCQKCPKKQSYHGY
  TQMMECLQGYKRVNNTFCQDINECQLQGVCPNGECLNTMGSYRCSCKMGFGPDPTFSSCVPDPPVISEEK
  GPCYRLVSPGRHCMHPLSVHLTKQICCCSVGKAWGPHCEKCPLPGTAAFKEICPGGMGYTVSGVHRRRPI
  HQHIGKEAVYVKPKNTQPVAKSTHPPPLPAKEEPVEALTSSWEHGPRGAEPEVVTAPPEKEIPSLDQEKT
  RLEPGQPQLSPGVSTIHLHPQFPVVVEKTSPPVPVEVAPEASTSSASQVIAPTQVTEINECTVNPDICGA
  GHCINLPVRYTCICYEGYKFSEQLRKCVDIDECAQVRHLCSQGRCENTEGSFLCVCPAGFMASEEGTNCI
  DVDECLRPDMCRDGRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPEEQCVNTPGSYQCVPCT
  EGFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHRGYSPTPDHRHCQDIDECQQGNLCMNGQCRN
  TDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCSHGQCRNTEGSFQCVCNQGYRASVLGDHCEDINECL
  EDSSVCQGGDCINTAGSYDCTCPDGFQLNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSIS
  ADGRTCEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGEAFCENVE
  GSFLCVCADENQEYSPMTGQCRSRVTEDSGVDRQPREEKKECYYNLNDASLCDNVLAPNVTKQECCCTSG
  AGWGDNCEIFPCPVQGTAEFTEMCPRGKGLVPAGESSYDTGGENYKDADECLLFGEEICKNGYCLNTQPG
  YECYCKQGTYYDPVKLQCFDMDECQDPNSCIDGQCVNTEGSYNCFCTHPMVLDASEKRCVQPTESNEQIE
  ETDVYQDLCWEHLSEEYVCSRPLVGKQTTYTECCCLYGEAWGMQCALCPMKDSDDYAQLCNIPVTGRRRP
  YGRDALVDFSEQYGPETDPYFIQDRFLNSFEELQAEECGILNGCENGRCVRVQEGYTCDCFDGYHLDMAK
  MTCVDVNECSELNNRMSLCKNAKCINTEGSYKCLCLPGYIPSDKPNYCTPLNSALNLDKESDLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_206958
• ORF Size: 4182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206958.3
• RefSeq Size: 6771 bp
• RefSeq ORF: 4185 bp
• Locus ID: 268977
• UniProt ID: Q8CG19
• Cytogenetics: 17 E2
• MW: 153.6 kDa
• Gene Summary: Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Outcompeted by LRRC32/GARP for binding to LAP regulatory chain of TGF-beta.[UniProtKB/Swiss-Prot Function]