Ltbp1 (NM_206958) Mouse Untagged Clone

CAT#: MC224375

Ltbp1 (untagged) - Mouse latent transforming growth factor beta binding protein 1 (Ltbp1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ltbp1
• Synonyms: 9430031G15Rik; 9830146M04; b2b1000Clo; Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224375 representing NM_206958
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACTAAGCTGATGTGTTTGTTGTTCTTTCTCTGTCTGCCTCTACTCCTAGTGAGTAACCACACTG
  GTCGCATCAAGGTGGTCTTTACTCCGAGCATCTGTAAAGTGACCTGCACCAAGGGCAACTGTCAGAACAG
  CTGCCAGAAGGGGAATACCACCACTCTCATTAGTGAGAACGGCCATGCAGCCGACACCCTGACAGCCACG
  AACTTCCGAGTGGTTATTTGCCATCTTCCGTGTATGAATGGTGGCCAGTGCAGTTCAAGGGACAAATGTC
  AGTGCCCTCCAAATTTCACAGGAAAGCTTTGCCAGATCCCTGTCCTTGGTGCCAGTATGCCCAAACTCTA
  CCAGCACGCCCAGCAGCAGGGCAAGGCATTGGGGAGTCACGTCATCCATTCCACACATACCTTGCCTCTA
  ACCATGACTAGCCAGCAAGGGGTCAAAGTGAAATTCCCCCCCAACATAGTCAATATCCATGTGAAACATC
  CTCCCGAGGCTTCTGTCCAGATACACCAGGTTTCCAGGATTGACAGCCCAGGAGGCCAGAAGGTGAAGGA
  AGCCCAACCAGGCCAGTCCCAAGTCTCTTACCAAGGACTTCCGGTCCAGAAGACCCAGACAGTCCATTCT
  ACGTACTCCCACCAGCAGCTTATTCCCCATGTGTATCCCGTGGCTGCTAAGACACAACTTGGCCGATGCT
  TCCAGGAAACCATTGGATCACAGTGTGGCAAAGCGCTCCCTGGCCTTTCCAAGCAAGAGGACTGCTGTGG
  GACAGTGGGGACGTCCTGGGGCTTTAACAAATGCCAGAAGTGCCCCAAGAAACAATCCTATCATGGATAT
  ACTCAAATGATGGAATGCCTACAAGGATATAAACGGGTTAACAACACCTTTTGTCAAGATATTAATGAAT
  GTCAGCTACAAGGTGTATGCCCTAATGGTGAGTGTTTGAATACCATGGGCAGCTACAGATGCTCCTGCAA
  AATGGGATTCGGGCCTGACCCTACCTTTTCAAGTTGTGTTCCGGATCCCCCTGTGATCTCAGAAGAGAAA
  GGGCCCTGTTACCGCCTTGTCAGTCCTGGAAGGCATTGTATGCACCCTCTCTCTGTTCATCTCACCAAGC
  AGATCTGCTGTTGTAGTGTGGGCAAGGCCTGGGGCCCACATTGTGAGAAATGTCCCCTCCCAGGCACAGC
  TGCTTTTAAGGAAATCTGTCCTGGTGGAATGGGTTATACGGTTTCTGGCGTTCATAGACGCAGGCCAATC
  CATCAGCATATAGGTAAAGAAGCTGTATATGTCAAGCCAAAGAACACTCAACCTGTTGCTAAAAGTACTC
  ATCCTCCACCTCTCCCAGCCAAGGAAGAGCCTGTGGAGGCCCTGACCTCCTCGTGGGAACACGGGCCGAG
  AGGGGCGGAGCCCGAAGTGGTAACAGCACCTCCTGAGAAGGAAATACCTTCACTGGATCAAGAGAAAACC
  AGACTTGAACCTGGGCAACCCCAGCTCTCCCCAGGGGTTTCAACTATTCATCTGCACCCACAGTTTCCAG
  TAGTGGTTGAAAAGACATCCCCTCCTGTGCCTGTGGAAGTAGCTCCTGAAGCTTCCACATCCAGTGCCAG
  CCAAGTGATTGCTCCTACGCAAGTAACAGAAATCAACGAATGTACTGTGAACCCGGACATTTGCGGAGCA
  GGACACTGCATCAACCTGCCGGTGCGGTATACCTGCATATGCTACGAGGGCTACAAGTTCAGTGAACAGC
  TGAGGAAATGTGTCGACATTGATGAATGTGCTCAGGTCCGGCACCTCTGCTCCCAGGGTCGCTGTGAGAA
  CACAGAGGGAAGTTTCCTGTGTGTCTGCCCGGCAGGGTTTATGGCCAGTGAGGAGGGTACTAACTGTATA
  GATGTGGATGAGTGCCTGAGGCCTGATATGTGTAGGGATGGCCGCTGCATCAACACCGCTGGGGCCTTCC
  GGTGCGAGTACTGTGACAGTGGGTACCGGATGTCACGGAGAGGCTACTGTGAGGATATTGATGAGTGTCT
  GAAGCCAAGCACTTGTCCCGAGGAACAGTGTGTGAACACCCCGGGCTCTTACCAGTGTGTGCCCTGCACA
  GAGGGGTTCCGCGGCTGGAATGGACAATGCCTTGATGTGGACGAGTGCCTGCAGCCAAAGGTCTGTACGA
  ATGGTTCCTGCACCAACCTGGAAGGGTCCTACATGTGTTCCTGCCACAGGGGCTACAGCCCCACACCAGA
  CCATAGACACTGTCAAGATATTGATGAATGCCAACAAGGGAATCTCTGCATGAATGGGCAGTGCAGAAAT
  ACCGATGGCTCCTTCCGGTGTACCTGTGGACAGGGCTATCAGCTGTCAGCAGCTAAAGACCAATGTGAAG
  ATATTGATGAATGCGAGCACCATCACCTCTGCTCTCACGGGCAGTGCAGGAACACAGAGGGCTCCTTCCA
  GTGTGTGTGCAACCAGGGTTACAGAGCATCTGTACTTGGAGACCACTGTGAGGATATCAATGAATGCTTG
  GAGGACAGCAGTGTCTGCCAGGGAGGTGACTGCATCAATACTGCGGGGTCCTATGATTGCACATGCCCGG
  ATGGATTCCAGCTGAACGACAATAAGGGCTGTCAAGACATTAATGAATGTGCACAGCCAGGACTCTGTGG
  ATCTCATGGGGAGTGTCTAAACACACAAGGCTCCTTCCACTGTGTCTGTGAACAGGGGTTCTCCATCTCT
  GCCGATGGCCGTACATGCGAAGATATTGATGAGTGTGTTAACAACACTGTGTGTGACAGTCACGGCTTCT
  GTGACAACACAGCCGGCTCTTTCCGCTGCCTCTGTTATCAGGGCTTTCAAGCCCCACAGGATGGGCAAGG
  GTGTGTGGATGTGAACGAGTGTGAACTGCTCAGTGGTGTATGTGGGGAAGCCTTCTGTGAAAACGTGGAA
  GGATCCTTCCTGTGTGTGTGTGCCGATGAGAACCAGGAGTACAGCCCCATGACTGGGCAGTGTCGCTCTC
  GGGTCACGGAAGATTCAGGTGTGGATCGTCAGCCTAGAGAAGAGAAGAAGGAGTGCTATTATAACCTCAA
  TGATGCTAGTCTCTGTGATAATGTCCTGGCCCCCAACGTCACCAAACAAGAGTGCTGCTGTACGTCAGGT
  GCCGGCTGGGGAGACAACTGTGAGATCTTCCCTTGCCCCGTCCAGGGAACTGCTGAGTTCACGGAAATGT
  GCCCTAGAGGGAAAGGTTTGGTCCCGGCTGGAGAATCCTCTTACGACACTGGTGGCGAGAACTACAAAGA
  TGCTGATGAATGCTTGCTGTTTGGAGAGGAAATCTGTAAAAATGGTTACTGTTTGAACACTCAACCTGGG
  TATGAATGCTACTGTAAGCAAGGGACATACTATGATCCTGTCAAATTACAGTGCTTTGATATGGATGAAT
  GCCAAGACCCTAACAGTTGCATTGACGGCCAGTGTGTGAACACAGAGGGCTCCTACAACTGCTTTTGCAC
  CCACCCAATGGTCCTGGATGCCTCTGAGAAGAGATGTGTGCAACCAACTGAATCAAATGAACAAATAGAA
  GAGACCGATGTCTATCAAGATCTGTGCTGGGAGCATCTGAGTGAGGAGTACGTGTGTAGCCGTCCTCTCG
  TAGGCAAGCAGACGACGTACACAGAATGCTGCTGTTTGTACGGGGAGGCTTGGGGCATGCAGTGTGCTCT
  GTGCCCCATGAAGGACTCAGATGACTATGCCCAGCTGTGCAATATCCCTGTGACTGGGCGTCGGCGGCCA
  TATGGACGGGATGCATTGGTGGACTTCAGTGAACAGTATGGCCCAGAAACAGACCCTTACTTCATCCAGG
  ATCGCTTTCTAAATAGCTTTGAGGAGCTACAGGCTGAGGAGTGTGGCATCCTCAATGGTTGTGAAAACGG
  CCGCTGTGTGAGAGTCCAGGAAGGATACACTTGCGATTGCTTTGATGGATATCACCTGGATATGGCCAAG
  ATGACCTGTGTCGATGTAAATGAATGCAGCGAGCTGAATAATCGGATGTCTCTCTGCAAGAACGCCAAGT
  GCATTAACACAGAAGGCTCCTACAAATGCTTGTGTCTGCCAGGCTACATACCATCCGACAAGCCCAACTA
  CTGTACACCCCTGAACAGCGCTTTGAATTTAGACAAAGAAAGTGACCTGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_206958
• Insert Size: 4185 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206958.2, NP_996841.1
• RefSeq Size: 6771 bp
• RefSeq ORF: 4185 bp
• Locus ID: 268977
• UniProt ID: Q8CG19
• Cytogenetics: 17 E2
• Gene Summary: Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Outcompeted by LRRC32/GARP for binding to LAP regulatory chain of TGF-beta.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and has an alternate 5' coding segment, compared to variant 1. The encoded isoform (LTBP-1S) has a shorter and distinct N-terminus when compared to isoform LTBP-1L. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.