Brpf1 (NM_030178) Mouse Tagged ORF Clone

CAT#: MR211850

Brpf1 (Myc-DDK-tagged) - Mouse bromodomain and PHD finger containing, 1 (Brpf1)

Product Images

Circular map for MR211850

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brpf1
• Synonyms: 4833438B11Rik; 4930540D11Rik; Brpf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211850 representing NM_030178
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTGGACTTTGACGTGAAGACCTTCTGCCACAATTTGCGGGCAACTAAGCCACCATATGAGTGCC
  CTGTGGAGACTTGCCGCAAGGTTTACAAGAGTTACAGTGGTATCGAGTACCACCTGTACCACTATGACCA
  CGACAGCCCACCACCCCCACAGCAGACCCCACTGCGCAAGCACAAAAAGAAAGGGCGCCAGTCACGACCA
  GCCAACAAGCAGTCACCCAGCCCCTCTGAAGTCTCACAGTCACCAGGCCGAGAGGTGATGAGCTATGCTC
  AGGCCCAGCGCATGGTAGAAGTGGACCTTCATGGCCGTGTCCACCGAATCAGCATCTTTGACAACTTGGA
  TGTGGTGTCAGAGGATGAGGAGGCCCCTGAGGAGGCCCCTGAGAATGGCAGCAACAAGGAAAACACTGAG
  ACACCTGCGGCTACACCTAAGTCAGGCAAGCATAAGAACAAGGAGAAACGAAAAGACTCTAACCACCACC
  ATCACAGCGCTCCTGCCAGTGCTGCTCCCAAATTGCCTGAGGTGGTGTATCGTGAGCTAGAGCAAGATAC
  CCCTGACGCACCACCCCGGCCCACTTCCTACTACCGGTACATCGAGAAATCTGCAGAGGAGCTGGATGAG
  GAGGTGGAGTATGACATGGATGAAGAGGACTATATCTGGCTGGATATCATGAATGAGCGGCGGAAGACTG
  AGGGTGTAAGTCCCATCCCACAAGAGATCTTTGAGTACTTAATGGACCGTTTGGAGAAGGAGTCGTACTT
  TGAGAGTCACAATAAAGGTGACCCCAATGCACTAGTGGATGAAGATGCCGTGTGCTGTATCTGCAATGAT
  GGCGAGTGCCAGAACAGCAATGTTATCCTCTTCTGTGACATGTGTAACTTGGCTGTGCACCAGGAGTGCT
  ACGGTGTCCCCTATATCCCTGAAGGCCAGTGGCTGTGCCGCCGTTGCCTGCAGTCACCTTCTCGTGCAGT
  GGATTGTGCTCTGTGCCCCAATAAGGGTGGTGCCTTCAAGCAGACAGATGATGGCCGCTGGGCCCACGTG
  GTGTGTGCCTTGTGGATCCCTGAGGTTTGCTTTGCCAACACAGTCTTCCTAGAACCTATTGACAGCATTG
  AGCACATCCCACCAGCTCGGTGGAAGCTCACCTGCTACATTTGTAAACAGCGGGGCTCTGGAGCCTGCAT
  CCAGTGCCATAAGGCCAATTGCTACACAGCCTTCCATGTGACATGTGCCCAACAAGCTGGCCTTTACATG
  AAGATGGAACCTGTGCGGGAGACAGGTGCCAATGGTACCTCCTTTAGCGTCCGCAAGACAGCCTACTGTG
  ACATCCACACACCCCCAGGTTCTGCTCGTCGCCTGCCTGCCCTATCCCACAGTGAGGGTGAGGAAGAAGA
  GGATGAAGAAGAAGATGAGGGTAAAAGCTGGAGCTCAGAGAAGGTCAAGAAGGCCAAGGCCAAGTCTCGG
  ATTAAGATGAAGAAAGCTCGGAAGATCTTGGCAGAGAAGAGGGCAGCAGCACCTGTGGTGTCCGTGCCCT
  GCATCCCGCCACACAGGCTCAGTAAGATCACCAACCGCCTGACCATCCAGAGGAAGAGCCAGTTCATGCA
  GAGGCTGCACAGCTACTGGACTCTGAAACGACAATCACGGAATGGGGTCCCACTACTCAGGCGCCTACAA
  ACACACCTTCAGTCTCAGAGGAACTGTGAACAAGTTGGGAGAGATTCTGATGATAAAAACTGGGCCCTCA
  AAGAACAGCTCAAGTCCTGGCAGAGACTGCGGCATGACCTGGAGCGAGCTCGGCTGCTGGTGGAGCTGAT
  CCGCAAGCGAGAGAAACTAAAAAGGGAGACGATCAAGATCCAGCAGATTGCCATGGAGATGCAGCTGACC
  CCTTTCCTCATCCTCCTCCGAAAAACCTTGGAGCAGCTCCAAGAGAAGGACACAGGCAACATCTTCAGCG
  AGCCGGTCCCTCTGTCTGAGGTACCCGACTACCTAGACCACATCAAAAAGCCCATGGACTTTTTCACCAT
  GAAGCAGAACTTGGAGGCTTACCGCTACTTGAACTTTGATGATTTTGAGGAGGACTTCAACCTCATTGTC
  AGCAACTGCCTAAAGTATAATGCCAAGGACACCATCTTCTACAGGGCAGCAGTGCGACTCCGTGAGCAGG
  GTGGTGCTGTGCTCCGTCAAGCCCGGCGACAGGCAGAAAAAATGGGCATTGACTTTGAGACGGGCATGCA
  TATCCCTCACAACCTGGCCGGAGATGAGGTCTCACACCACACTGAAGATGTAGAGGAAGAACGGCTGGTC
  CTGCTGGAGAACCAGAAACACCTGCCAGTAGAAGAACAGCTGAAGTTGTTGCTGGAGAGGCTGGATGAAG
  TCAATGCCAGCAAGCAGAGTGTGGGCCGCTCTCGGCGTGCAAAAATGATCAAGAAGGAGATGACAGCATT
  GCGGCGGAAGCTTGCTCACCAGCGGGAGACTGGCCGGGATGGGCCTGAGCGTCATGGCCCCTCAGGTCGG
  GGCAATCTGACACCCCACCCAGCAGCCTGTGACAAAGATGGACAGACCGACAGTGCTGCAGAAGAGAGCA
  GCAGCCAGGAAACAAGCAAAGGCCTGGGTCCCAACATGTCCTCAACCCCCGCACATGAGGTGGGCAGGAG
  AACCTCAGTTCTGTTCTCCAAAAAGAACCCGAAGACAGCTGGACCGCCCAAGAGGCCGGGCCGGCCCCCA
  AAAAACCGGGAGAGCCAGATGACCCCCAGCCACGGAGGCAGTCCTGTGGGGCCCCCTCAGCTCCCCATCA
  TGGGCTCCCTACGTCAGCGCAAGCGGGGTAGGAGCCCCCGGCCCAGTTCAAGCTCAGACAGCGACAGTGA
  TAAGTCCACAGAAGATCCCCCAATGGACTTACCAGCCAATGGCTTCAGCAGTGGGAACCAGCCAGTGAAG
  AAGAGTTTCTTGGTGTACCGTAATGACTGCAACCTTCCCCGAAGCAGCTCAGACTCTGAGTCCAGCAGCA
  GCAGCAGCAGCAGTGCAGCCTCAGACCGGACCAGCACAACTCCCTCAAAACAAGGCAGGGGCAAGCCCTC
  TTTCTCTCGGGGCACATTCCCAGAAGACAGTAGTGAAGATACCTCAGGCACTGAGAATGAGGCCTACTCC
  GTGGGCACTGGCCGCGGCGTGGGCCACAGCAGTAAGTACCCTCACCCAAAGTCAGGGGTGCTGGGGACCC
  AGTTTCAAGGCCTTGCCAGCCCCCCAGCTGCTGATCCGCCCCCTCTCTCCCGTTCCTGTGAAGTGGTAAG
  AAAGAGTCTGGGTCGAGGAGCTGGCTGGCTGTCAGAGGATGAGGACTCCCCGTTGGATGCTCTGGACCTC
  GTGTGGGCCAAATGCCGAGGCTATCCATCATACCCAGCTCTGATCATTGATCCAAAGATGCCCCGAGAAG
  GTATGTTCCACCATGGGGTTCCTATCCCTGTACCACCACTGGAGGTTCTAAAACTTGGGGAACAAATGAC
  ACAGGAAGCCCGAGAGCATCTCTACCTCGTTCTCTTCTTTGACAACAAACGAACCTGGCAGTGGCTGCCC
  CGGACTAAGCTTGTTCCTCTGGGTGTGAACCAGGATCTAGACAAAGAGAAGATGCTGGAGGGCCGCAAGT
  CCAACATCCGCAAGTCAGTGCAGATTGCTTACCACAGGGCTCTGCAGCACCGAAGCAAGGTGCAGGGTGA
  GCAGAGCAGCGAGACCAGCGATAGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211850 representing NM_030178
  Red=Cloning site Green=Tags(s)
  
  MGVDFDVKTFCHNLRATKPPYECPVETCRKVYKSYSGIEYHLYHYDHDSPPPPQQTPLRKHKKKGRQSRP
  ANKQSPSPSEVSQSPGREVMSYAQAQRMVEVDLHGRVHRISIFDNLDVVSEDEEAPEEAPENGSNKENTE
  TPAATPKSGKHKNKEKRKDSNHHHHSAPASAAPKLPEVVYRELEQDTPDAPPRPTSYYRYIEKSAEELDE
  EVEYDMDEEDYIWLDIMNERRKTEGVSPIPQEIFEYLMDRLEKESYFESHNKGDPNALVDEDAVCCICND
  GECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCPNKGGAFKQTDDGRWAHV
  VCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLYM
  KMEPVRETGANGTSFSVRKTAYCDIHTPPGSARRLPALSHSEGEEEEDEEEDEGKSWSSEKVKKAKAKSR
  IKMKKARKILAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRLQ
  THLQSQRNCEQVGRDSDDKNWALKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKIQQIAMEMQLT
  PFLILLRKTLEQLQEKDTGNIFSEPVPLSEVPDYLDHIKKPMDFFTMKQNLEAYRYLNFDDFEEDFNLIV
  SNCLKYNAKDTIFYRAAVRLREQGGAVLRQARRQAEKMGIDFETGMHIPHNLAGDEVSHHTEDVEEERLV
  LLENQKHLPVEEQLKLLLERLDEVNASKQSVGRSRRAKMIKKEMTALRRKLAHQRETGRDGPERHGPSGR
  GNLTPHPAACDKDGQTDSAAEESSSQETSKGLGPNMSSTPAHEVGRRTSVLFSKKNPKTAGPPKRPGRPP
  KNRESQMTPSHGGSPVGPPQLPIMGSLRQRKRGRSPRPSSSSDSDSDKSTEDPPMDLPANGFSSGNQPVK
  KSFLVYRNDCNLPRSSSDSESSSSSSSSAASDRTSTTPSKQGRGKPSFSRGTFPEDSSEDTSGTENEAYS
  VGTGRGVGHSSKYPHPKSGVLGTQFQGLASPPAADPPPLSRSCEVVRKSLGRGAGWLSEDEDSPLDALDL
  VWAKCRGYPSYPALIIDPKMPREGMFHHGVPIPVPPLEVLKLGEQMTQEAREHLYLVLFFDNKRTWQWLP
  RTKLVPLGVNQDLDKEKMLEGRKSNIRKSVQIAYHRALQHRSKVQGEQSSETSDSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030178
• ORF Size: 3738 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030178.2, NP_084454.1
• RefSeq Size: 4648 bp
• RefSeq ORF: 3741 bp
• Locus ID: 78783
• Cytogenetics: 6 E3
• MW: 140.8 kDa
• Gene Summary: Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Preferentially mediates histone H3-K23 acetylation (PubMed:27939640). Positively regulates the transcription of RUNX1 and RUNX2 (By similarity).[UniProtKB/Swiss-Prot Function]