Prag1 (NM_172911) Mouse Tagged Lenti ORF Clone
CAT#: MR211757L4
- LentiORF®
Lenti ORF clone of D8Ertd82e (mGFP-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e)
"NM_172911" in other vectors (4)
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Product Images
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Prag1 |
Synonyms | 9830148H23; D8Ertd82e; mFLJ00269; NACK; Sgk223 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR211757).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_172911 |
ORF Size | 4119 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172911.3, NP_766499.2 |
RefSeq Size | 4780 bp |
RefSeq ORF | 4122 bp |
Locus ID | 244418 |
UniProt ID | Q571I4 |
Cytogenetics | 8 22.78 cM |
Gene Summary | Catalytically inactive protein kinase that acts as a scaffold protein (By similarity). Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By similarity). Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (By similarity). Acts as a critical coactivator of Notch signaling (PubMed:25038227).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224352 | D8Ertd82e (untagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e), (10ug) |
USD 1,293.00 |
|
MG211757 | D8Ertd82e (tGFP-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e) |
USD 1,374.00 |
|
MR211757 | D8Ertd82e (Myc-DDK-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e) |
USD 1,174.00 |
|
MR211757L3 | Lenti ORF clone of D8Ertd82e (Myc-DDK-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e) |
USD 1,474.00 |
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