Kif5a (NM_008447) Mouse Tagged ORF Clone
CAT#: MR211477
- TrueORF®
Kif5a (Myc-DDK-tagged) - Mouse kinesin family member 5A (Kif5a), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_008447" in other vectors (4)
Interest in protein/lysate? Submit request here!
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Kif5a |
Synonyms | D10Bwg0738e; Khc; Kif5; Kns; mKIAA4086 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211477 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGACTAACAACGAATGCAGCATCAAGGTGCTTTGCCGATTTCGGCCCCTGAACCAGGCCGAGA TTCTGCGGGGGGACAAGTTCATCCCCATTTTCCAAGGGGACGACAGCGTCATTATTGGGGGAAAGCCATA TGTCTTTGACCGCGTCTTCCCCCCAAACACCACTCAGGAGCAGGTTTACCACGCCTGTGCCATGCAGATC GTCAAAGACGTCCTTGCTGGTTACAATGGCACAATCTTCGCTTATGGACAGACATCCTCAGGGAAAACGC ATACCATGGAGGGGAAGCTGCACGACCCTCAGCTGATGGGCATCATTCCCCGGATCGCTCGAGACATCTT CAACCACATCTACTCCATGGATGAGAACCTTGAATTCCACATTAAGGTATCTTACTTCGAGATTTACCTG GATAAGATCCGTGACCTTTTGGATGTGACCAAGACGAACCTGTCCGTGCATGAGGACAAAAACCGGGTGC CGTTTGTCAAGGGTTGTACCGAACGCTTTGTGTCCAGCCCAGAGGAGATTCTGGATGTGATCGATGAGGG GAAGTCCAACCGTCACGTAGCTGTCACCAACATGAACGAGCACAGTTCTCGGAGCCACAGCATCTTCCTC ATCAACATCAAGCAGGAGAACGTAGAGACCGAGCAGAAGCTCAGCGGGAAGCTGTACCTCGTGGATCTGG CCGGAAGCGAGAAGGTCAGCAAGACAGGGGCAGAGGGAGCCGTTCTGGACGAGGCAAAGAATATCAACAA GTCGCTGTCGGCCCTGGGGAACGTGATCTCTGCACTGGCAGAGGGCACCAAAAGCTACGTGCCGTACCGC GACAGCAAAATGACGAGGATTCTCCAGGACTCTCTGGGAGGGAACTGCAGGACTACCATGTTCATCTGCT GCTCGCCGTCCAGCTACAATGACGCAGAGACCAAGTCCACGCTCATGTTTGGACAGCGGGCGAAGACCAT CAAGAACACTGCCTCAGTGAATCTGGAGCTGACTGCTGAGCAGTGGAAGAAGAAGTATGAGAAGGAGAAG GAGAAGACCAAGGCCCAGAAGGAGACAATTGCGAAGCTAGAGGCTGAGCTTAGCCGGTGGCGCAATGGAG AGAATGTGCCTGAGACTGAGCGCCTGGCTGGAGAGGACTCAGCTCTGGGAGCTGAGCTCTGCGAGGAGAC CCCTGTGAATGACAACTCATCCATTGTGGTACGCATCGCACCTGAGGAAAGGCAGAAATATGAGGAAGAG ATCCGCCGTCTCTACAAGCAGCTTGATGACAAGGATGATGAGATCAACCAGCAGAGCCAGCTCATTGAGA AGCTGAAGCAGCAGATGCTGGACCAGGAAGAGCTGCTCGTGTCCACTCGGGGAGACAACGAGAAGGTCCA GCGGGAGCTTAGCCACCTGCAGTCCGAGAACGATGCTGCGAAGGACGAGGTGAAGGAAGTGCTGCAGGCC CTAGAGGAGCTGGCGGTCAACTACGACCAGAAGTCCCAGGAGGTGGAGGAGAAGAGCCAGCAGAACCAGC TGCTGGTGGACGAGCTGTCCCAGAAAGTGGCCACCATGCTGTCCCTGGAGTCCGAGCTACAGCGGCTCCA GGAGGTCAGTGGACACCAGCGAAAGCGGATCGCTGAGGTGCTGAATGGGCTGATGAGGGACCTGAGTGAG TTCAGTGTCATCGTGGGCAACGGCGAGATTAAGCTGCCCGTGGAGATCAGTGGGGCCATCGAGGAGGAGT TCACGGTGGCCCGGCTCTACATCAGCAAGATCAAGTCGGAGGTGAAGTCCGTGGTTAAGCGATGTCGGCA GCTGGAGAACCTCCAGGTGGAGTGTCATCGCAAGATGGAGGTGACCGGTAGGGAGCTGTCATCTTGCCAA CTGCTCATCTCACAGCATGAGGCCAAGATCCGTTCACTCACGGAGTACATGCAGACTGTGGAGTTGAAGA AACGGCACCTGGAAGAGTCCTACGACTCCCTGAGCGATGAGCTTGCCAGGCTCCAGGCGCACGAAACTGT ACACGAGGTAGCTCTGAAAGACAAGGAGCCAGACACACAGGACGCGGAGGAGGTGAAGAAGGCCCTGGAA CTACAGATGGAGAATCACCGTGAGGCCCATCACCGGCAGCTGGCCCGCCTCCGGGATGAGATTAATGAGA AACAGAAAACCATTGATGAGCTGAAAGACCTGAACCAGAAGCTCCAGTTAGAGCTGGAGAAGCTTCAGGC CGACTATGAGAGGCTGAAGAATGAAGAGAACGAGAAGAGCGCCAAGCTCCAGGAGCTGACATTTCTGTAT GAGCGACATGAGCAGTCCAAGCAGGACCTCAAGGGGCTGGAGGAGACAGTTGCCCGTGAACTCCAGACCC TCCACAACCTTCGCAAGCTGTTCGTTCAAGACGTCACGACTCGAGTCAAGAAAAGTGCAGAAATGGAGCC CGAGGACAGTGGGGGGATTCATTCCCAAAAGCAGAAGATCTCCTTTCTTGAGAACAACCTGGAACAGCTT ACAAAGGTTCACAAACAGCTGGTACGTGACAATGCAGATCTGCGTTGTGAGCTTCCTAAATTGGAAAAAC GACTTCGGGCTACGGCTGAGAGAGTTAAGGCCCTGGAGGGTGCACTGAAGGAGGCCAAGGAGGGCGCTAT GAAGGACAAGCGTAGATACCAGCAGGAGGTGGACCGCATCAAAGAAGCCGTGCGGTACAAGAGCTCCGGC AAGCGGGGCCATTCTGCCCAGATCGCTAAGCCTGTGAGGCCTGGCCACTATCCTGCCTCCTCACCCACCA ACCCCTACGGCACCCGGAGCCCCGAGTGTATCAGCTACACCAACAACCTCTTCCAGAACTACCAGAACCT GCACCTGCAGGCTGCGCCTAGCTCCACTTCAGATATGTACTTTGCCAGCAGCGGAGCCACATCTGTTGCC CCCTTGGCTTCCTACCAGAAGGCCAACATGGACAATGGAAATGCCACAGATATCAACGACAACAGGAGTG ACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTTTCCCTCTCCACCAAGAGACAGCAGCCAG C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211477 protein sequence
Red=Cloning site Green=Tags(s) MAETNNECSIKVLCRFRPLNQAEILRGDKFIPIFQGDDSVIIGGKPYVFDRVFPPNTTQEQVYHACAMQI VKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYSMDENLEFHIKVSYFEIYL DKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFL INIKQENVETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYR DSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEK EKTKAQKETIAKLEAELSRWRNGENVPETERLAGEDSALGAELCEETPVNDNSSIVVRIAPEERQKYEEE IRRLYKQLDDKDDEINQQSQLIEKLKQQMLDQEELLVSTRGDNEKVQRELSHLQSENDAAKDEVKEVLQA LEELAVNYDQKSQEVEEKSQQNQLLVDELSQKVATMLSLESELQRLQEVSGHQRKRIAEVLNGLMRDLSE FSVIVGNGEIKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQ LLISQHEAKIRSLTEYMQTVELKKRHLEESYDSLSDELARLQAHETVHEVALKDKEPDTQDAEEVKKALE LQMENHREAHHRQLARLRDEINEKQKTIDELKDLNQKLQLELEKLQADYERLKNEENEKSAKLQELTFLY ERHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTTRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQL TKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKSSG KRGHSAQIAKPVRPGHYPASSPTNPYGTRSPECISYTNNLFQNYQNLHLQAAPSSTSDMYFASSGATSVA PLASYQKANMDNGNATDINDNRSDLPCGYEAEDQAKLFPLHQETAAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_008447 |
ORF Size | 3084 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008447.4 |
RefSeq Size | 4304 bp |
RefSeq ORF | 3084 bp |
Locus ID | 16572 |
UniProt ID | P33175 |
Cytogenetics | 10 74.5 cM |
MW | 117 kDa |
Gene Summary | Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL) (PubMed:12682084). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons (PubMed:21976701). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205289 | Kif5a (untagged) - Mouse kinesin family member 5A (Kif5a), transcript variant 2, (10ug) |
USD 895.00 |
|
MG211477 | Kif5a (tGFP-tagged) - Mouse kinesin family member 5A (Kif5a), transcript variant 1 |
USD 1,095.00 |
|
MR211477L3 | Lenti ORF clone of Kif5a (Myc-DDK-tagged) - Mouse kinesin family member 5A (Kif5a), transcript variant 2 |
USD 1,195.00 |
|
MR211477L4 | Lenti ORF clone of Kif5a (mGFP-tagged) - Mouse kinesin family member 5A (Kif5a), transcript variant 2 |
USD 1,195.00 |
{0} Product Review(s)
Be the first one to submit a review