Ccp110 (NM_182995) Mouse Tagged ORF Clone
CAT#: MR211433
- TrueORF®
Ccp110 (Myc-DDK-tagged) - Mouse RIKEN cDNA 6330503K22 gene (6330503K22Rik)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_182995" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ccp110 |
Synonyms | 6330503K22Rik; AA415922; AI427129; AW557948; CP110 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211433 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAATATGAGGAGTTCTGCGAGAAAGCACTTGGCCGAGCTCAGGAAGCATCGCTGTCCACAGGCA GCTTCCTGCCCGCTCAGGCAGAAAGTGTCTCACTCATCCGTTTCCATGGGGTGGCTGTACTTTCTCCACT GCTTACCATTGAGAAGAGAAAGAAAATTCAAGAGGAAAAGCAGAAAGCACTTGATGTGCAGTCAAGAAAG CAGGCCAACAGGAAGAAAGCCTTACTGACTCGTGTCCAGGAGATTCTTGAAAATGTTCAGGTTAGAAAAG CACCAAATGCCAGTGATTTTGATCAGTGGGCAACAGAAACCATTTACTCTAATCCAGAAGTCACAGACTT GAATGTTCCTGTTAGAGTTCCAAACAGCTTGCCGAGCCCCACCGAACACTGTACTTCAGTAAAGCTTGAA AAGATAACTGGACTTTTGCCTGTGAATAATGAGGACCAACAAACCCCTAAGCGGGTGGGGCTGCCTGGAG ACTCAGAGGTGTCTGGTTCTCTGAGGCAGTGTGAGAGTCCAGAAAGCAGGCAGGCAGAAGATGGAGCTGC TCTAAGGCTTTCTTCAGCAAGCCCACAGGAGACCATCATTTCTGATGTCTTAGGAAAGGAAGAACAGGAC CCATCATGTTTGGCTGAAGTCACTCCAGATCCCTACATAATGAGCCTTCAGAATCTAATGAAGAGGTCAA AGGAATACGTGGAAAGAGAACTGTCTAGCCGGAGTCTGAGAAATAGTTTAAAGAGAAGTGTTAATGAAAC GCATTCAGACAGAGAAAACGATGCTGCTAAGGCGAGCGACTGTGTAAAGGAGAAGGCCCCACCCATGCCC ATAGGCAGGCACTGTGGCTCTGCCATCCCTGACAAACCAAGCCTTAATAAGTCAAATGTTCTCCTCCAAG GTGCTTCACAAGCAAGCAGCATGGGCACGGCAGGCTTAGCTAGCTTTTCTAAGATAGACCTGCCTGCAGG AGCTGCTCCTCCCGCTGCTCCAGATGCCGGATCAGATTTTACAGTTATTCCTACCTTTGTTACTGAAAAT AAAGTTAAAAGTCTTAAAGGTCCATATGCCAAATTACCTAGTCCAGAGCCAAGTATGAGTCCTACGATGC ATCGGAGGCATTCTCGGTCAGCATCAGCATGTCAGATCCTTATAAACAACCCAGTAAATGCCTGTGAGCT GAGCCCAAAGGGGAAAGAAGAGGCAGTGGATAGAACCGCTCCAGCTGCTGCTGAGACAACAAATGAGTCT GAAACTGTGCCAAAATCACCGACTGATTTGACAGGGGTTTGTTCAAGTAATGTTTCTGCTACCAAAATCA CGTCTGAGAGCACAAGAGAGATGGTCGTGGGTAAGCCAAGTCAGAGGCAGCAGGCTTTAGGAGCTCACTT AGGCAATAACGTCACTGTTGAGCGGTCTGCTATGGAAGGTCCCTTCATAGCTGATGACAGGGGAGCCCAG AAGGTGGACGGTACTTGTATGGCTGTGCCCAAGTTGCATGAACTGCAGCCCAGCAGTCAGTGTGTGTCAA GTCAAACCTTGGAAGATGTGTGTGAACTCAAGTCAGCCAGTCTGCTAGCAAAGAACTCCTGCAACTTACA AATGGAACTGAATAAATCTTACGATGTGAAACACCCATCTCCCTTACTTACGCAAACCCAGACTTCCAGA CAGCAGATGGACACACCGCCGGTGTTCCGTGGAAACGAGCAGTTTGTAGATAACAGTTTTGAGAAAGTTA AGCGGAGACTTGATTTAGATGTTGATAGTTTACAAAAAGAAAATTGTCCTTATATTATAACAGCTGGAGT AGCTGAGCAGGAGCGGGACCGTCTGCTGGAAAGAAGATACCCCAAGGGATTCGTCCACATTAACAAGAAT AAGATGCTAGAAACTAGCCCCAAAGAAGGCCAGGAACTACTGAAAAGCAAGATGTTAGCTTTTGAAGAAA TGCGAAAGAGACTAGAGGAACAGCATGCCCAGCAGTTGTCACTCCTCATAGCTGAGCAGGAGAGGGAGCA GGAGCAACTGCAAAAGGAAATAGAAGAGCAGGAGAAGATGCTGAAGGAGAAGGCAGTTACGACGGACGTC TCTGACTTGAACAGTGCACTGGAGTGGAGACAAAGAACCGATTCTGCTTTGCTAGAAACCATGCTGTCCC AAGTGGACTCACTCCAGACTTCAAACAACTCTGGCTTCATAACTTCTGCCCTGCAGTACAGCTTCGGCTC TGCAGGTGAAGCACCGTTCTACCTCTGGGGATCACTGACCAGTGGCGTGACCAGAGTCTCAGGAACAAGG CCTTGTGGACGGGCCCAAGCTAAATGGTCTCAGGTTTTTAATCCAGAAATACACGCAAAATTTAATAAAA TAACTGCCGTGGCAAAAGGGTTTCTTACTCGTAAACTTATGCAAACAGACAAACTGAAGCAGCTTCGACA AACTGTAAAAGACACCATGGAATTCATACGAAGTTTTCAGTCAGAAGCCCCTCTGAAGAGAGGTGTCGTC TCAGCCCAAGATGCTTCCCTTCAGGAACGAGTGTTAGCTCAGTTGCGAGCTGCTCTGTATGGTATTCATG ACATCTTCTTCGTAATGGATGCAGCCGAGAGAATGTCAATCCTCCATCATGATCGAGAAGCCCGCAAAGA GAAACTGCTCCGGCAAATGGATAAGATGAAAAGTCCACGAGTGGCGCTTTCAGTGGCAACACAGAAGTCC CTGGACAGGAAGAAGTTCATGAAAGTTGCTGAGATGGGAATGCCAAATAAGAAGTTCCTGCTTAAACAAA ACCCCTCAGAAACAAGAGTCCTTCAGCCAAACCAAGGACAGAATGCACCCGTTCATAGGCTACTTAGTAG ACAAGGAACCCCTAAGACTTCAGTGAAGGGGGTTGTGCAAAATAGACAGAAGCCTTCACAGAGCAGAGTG CCTAACAGAGCGCCTGTGTCAGGAGCATATGCAGGAAAAACCCAAAGAAAGCGGCCAAATGTTGCGACAA TT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211433 protein sequence
Red=Cloning site Green=Tags(s) MEEYEEFCEKALGRAQEASLSTGSFLPAQAESVSLIRFHGVAVLSPLLTIEKRKKIQEEKQKALDVQSRK QANRKKALLTRVQEILENVQVRKAPNASDFDQWATETIYSNPEVTDLNVPVRVPNSLPSPTEHCTSVKLE KITGLLPVNNEDQQTPKRVGLPGDSEVSGSLRQCESPESRQAEDGAALRLSSASPQETIISDVLGKEEQD PSCLAEVTPDPYIMSLQNLMKRSKEYVERELSSRSLRNSLKRSVNETHSDRENDAAKASDCVKEKAPPMP IGRHCGSAIPDKPSLNKSNVLLQGASQASSMGTAGLASFSKIDLPAGAAPPAAPDAGSDFTVIPTFVTEN KVKSLKGPYAKLPSPEPSMSPTMHRRHSRSASACQILINNPVNACELSPKGKEEAVDRTAPAAAETTNES ETVPKSPTDLTGVCSSNVSATKITSESTREMVVGKPSQRQQALGAHLGNNVTVERSAMEGPFIADDRGAQ KVDGTCMAVPKLHELQPSSQCVSSQTLEDVCELKSASLLAKNSCNLQMELNKSYDVKHPSPLLTQTQTSR QQMDTPPVFRGNEQFVDNSFEKVKRRLDLDVDSLQKENCPYIITAGVAEQERDRLLERRYPKGFVHINKN KMLETSPKEGQELLKSKMLAFEEMRKRLEEQHAQQLSLLIAEQEREQEQLQKEIEEQEKMLKEKAVTTDV SDLNSALEWRQRTDSALLETMLSQVDSLQTSNNSGFITSALQYSFGSAGEAPFYLWGSLTSGVTRVSGTR PCGRAQAKWSQVFNPEIHAKFNKITAVAKGFLTRKLMQTDKLKQLRQTVKDTMEFIRSFQSEAPLKRGVV SAQDASLQERVLAQLRAALYGIHDIFFVMDAAERMSILHHDREARKEKLLRQMDKMKSPRVALSVATQKS LDRKKFMKVAEMGMPNKKFLLKQNPSETRVLQPNQGQNAPVHRLLSRQGTPKTSVKGVVQNRQKPSQSRV PNRAPVSGAYAGKTQRKRPNVATI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_182995 |
ORF Size | 3015 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_182995.2 |
RefSeq Size | 5024 bp |
RefSeq ORF | 3015 bp |
Locus ID | 101565 |
UniProt ID | Q7TSH4 |
Cytogenetics | 7 F2 |
MW | 111.1 kDa |
Gene Summary | Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation (PubMed:23141541). Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation (PubMed:26965371). Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223165 | Ccp110 (untagged) - Mouse RIKEN cDNA 6330503K22 gene (6330503K22Rik), (10ug) |
USD 962.00 |
|
MG211433 | Ccp110 (tGFP-tagged) - Mouse RIKEN cDNA 6330503K22 gene (6330503K22Rik) |
USD 1,075.00 |
|
MR211433L3 | Lenti ORF clone of Ccp110 (Myc-DDK-tagged) - Mouse RIKEN cDNA 6330503K22 gene (6330503K22Rik) |
USD 1,175.00 |
|
MR211433L4 | Lenti ORF clone of Ccp110 (mGFP-tagged) - Mouse RIKEN cDNA 6330503K22 gene (6330503K22Rik) |
USD 1,175.00 |
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