Atp2a3 (NM_001163336) Mouse Tagged ORF Clone

CAT#: MR211414

Atp2a3 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 1

Product Images

Circular map for MR211414

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp2a3
• Synonyms: Serca3; SERCA3b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211414 representing NM_001163336
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGCGCACCTGCTCTCCGCTGCCGACGTGCTGCGTCGCTTCTCGGTGACAGCCGAGGGCGGCT
  TGAGCCTGGAGCAGGTGACCGACGCGCGGGAGCGTTACGGCCCCAACGAGCTCCCGACCGAGGAAGGGAA
  ATCCCTGTGGGAGCTGGTGGTGGAGCAGTTTGAGGACCTCTTGGTGCGCATCCTGCTGCTGGCAGCTCTG
  GTTTCCTTTGTCCTGGCCTGGTTCGAGGAGGGTGAGGAGACCACAACGGCCTTCGTGGAGCCCCTGGTCA
  TCATGCTAATTCTCGTGGCCAATGCGATCGTGGGAGTGTGGCAGGAACGCAACGCAGAGAGTGCAATTGA
  GGCCTTGAAGGAGTATGAGCCTGAGATGGGTAAGGTGATCCGGTCTGACCGCAAGGGTGTACAGAGGATC
  CGAGCCAGGGACATCGTCCCTGGGGACATTGTGGAAGTGGCAGTGGGAGACAAGGTACCCGCTGACCTCC
  GTCTCATCGAGATCAAGTCCACCACACTTCGAGTGGACCAGTCCATCCTGACAGGGGAATCCGTGTCTGT
  GACGAAGCACACAGATGCCATTCCAGACCCCCGAGCCGTGAACCAGGACAAGAAGAACATGCTATTTTCT
  GGCACCAATATTGCATCAGGCAAAGCCCTGGGTGTGGCTGTGGCTACAGGCCTGCAGACAGAGCTGGGCA
  AGATTCGGAGCCAGATGGCGGCTGTGGAGCCTGAGCGGACGCCATTGCAGCGCAAGCTGGATGAATTTGG
  GAGGCAGCTGTCCCATGCCATCTCTGTGATCTGTGTGGCTGTGTGGGTCATCAACATCGGCCACTTTGCT
  GACCCGGCCCACGGTGGATCCTGGCTCCGTGGTGCAGTCTACTACTTCAAGATCGCTGTGGCCCTGGCTG
  TGGCTGCTATCCCCGAGGGTCTCCCAGCAGTCATCACTACATGCCTGGCCCTGGGCACAAGACGTATGGC
  ACGTAAGAATGCCATTGTGCGGAGCCTGCCTTCTGTGGAGACGCTGGGCTGCACCTCGGTCATCTGCTCT
  GACAAGACGGGGACGCTCACCACCAATCAGATGTCTGTCTGCAGGATGTTCGTGGTGGCTGAAGCAGAGG
  CAGGCACCTGTCGTTTGCATGAATTCACCATTTCGGGTACCACGTACACCCCAGAGGGCGAAGTGAGGCA
  AGGGGAGCAGCCTGTGCGCTGTGGGCAGTTTGATGGGCTAGTGGAGCTCGCCACCATCTGTGCCCTCTGC
  AATGATTCTGCGCTGGACTACAATGAGGCCAAGGGTGTGTATGAGAAGGTAGGAGAGGCCACAGAGACCG
  CCCTGACTTGCCTGGTAGAGAAGATGAATGTGTTTGACACAGACCTGAAAGGACTGTCGCGGGTGGAGCG
  TGCTGGCGCCTGCAACTCAGTCATCAAGCAACTCATGCGGAAAGAGTTCACCCTGGAGTTCTCCCGGGAC
  CGGAAGTCTATGTCCGTGTACTGCACACCTACTCGTGCTGACCCCAAGGTCCAGGGCAGCAAGATGTTTG
  TGAAGGGAGCTCCTGAGAGTGTAATTGAGCGCTGTAGCTCAGTCCGAGTGGGCAGCCGAACAGCACCCCT
  GAGCACCACTTCCAGAGAGCATATCCTGGCCAAGATCCGGGACTGGGGCTCAGGCTCTGACACACTGCGC
  TGCTTAGCCTTGGCCACCAGGGACACACCCCCAAGGAAGGAGGACATGCATCTAGATGACTGCAGCCGGT
  TTGTACAGTACGAGACAGACCTGACCTTCGTGGGCTGCGTGGGCATGTTAGACCCACCGAGACCAGAGGT
  GGCTGCTTGTATCACACGCTGTTCCCGGGCTGGCATTCGAGTGGTCATGATCACAGGGGACAACAAAGGG
  ACAGCTGTGGCCATCTGCCGCCGACTTGGCATCTTTGGGGACACGGAGGATGTGTTGGGCAAGGCCTACA
  CGGGCCGTGAGTTTGATGACCTCAGCCCAGAGCAGCAGCGCCAGGCTTGTCGCACTGCCCGGTGCTTTGC
  CCGAGTGGAACCCGCACATAAGTCTCGAATCGTGGAGAACCTACAGTCCTTTAATGAGATCACTGCCATG
  ACTGGCGATGGGGTGAATGATGCACCAGCCCTGAAGAAGGCAGAGATCGGCATCGCCATGGGCTCAGGCA
  CTGCTGTGGCCAAGTCAGCAGCAGAGATGGTGCTGTCCGATGACAACTTTGCCTCCATTGTGGCTGCAGT
  AGAAGAGGGCAGGGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATCTCCTCCAATGTTGGC
  GAGGTTGTCTGCATCTTCCTCACAGCAATTCTGGGCCTGCCCGAAGCTCTGATCCCTGTGCAGCTGCTCT
  GGGTGAACCTGGTAACAGATGGCTTACCGGCCACAGCCCTTGGCTTCAACCCACCAGATCTGGATATCAT
  GGAGAAGCCGCCTCGGAATCCCCGTGAGGCCCTCATCAGTGGCTGGCTTTTTTTCCGCTATTTGGCTATT
  GGAGTGTATGTAGGCCTGGCTACAGTGGCTGCCGCCACCTGGTGGTTCCTGTATGACACCGAGGGACCTC
  AAGTCACCTTCTATCAGCTGAGGAACTTCCTGAAGTGCTCTGAAGACAATCCACTGTTTGCTGGCATTGA
  CTGCAAGGTTTTTGAGTCACGCTTCCCCACAACCATGGCCTTATCTGTGCTTGTGACCATTGAAATGTGC
  AACGCCCTCAACAGCGTCTCTGAGAACCAGTCACTGCTGCGCATGCCACCCTGGCTGAACCCTTGGCTGC
  TGGGGGCTGTGGTCATGTCCATGGCTCTGCATTTTCTTATCCTCCTGGTGCCACCTCTGCCTCTCATTTT
  TCAGGTGACCCCACTGAGTGGTCGGCAGTGGGGGGTGGTGCTTCAGATGTCTCTGCCTGTCATCCTGCTC
  GATGAGGCCCTCAAGTATCTGTCCAGGAATCACATGGATGAAAAAAAGGACCTGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211414 representing NM_001163336
  Red=Cloning site Green=Tags(s)
  
  MEEAHLLSAADVLRRFSVTAEGGLSLEQVTDARERYGPNELPTEEGKSLWELVVEQFEDLLVRILLLAAL
  VSFVLAWFEEGEETTTAFVEPLVIMLILVANAIVGVWQERNAESAIEALKEYEPEMGKVIRSDRKGVQRI
  RARDIVPGDIVEVAVGDKVPADLRLIEIKSTTLRVDQSILTGESVSVTKHTDAIPDPRAVNQDKKNMLFS
  GTNIASGKALGVAVATGLQTELGKIRSQMAAVEPERTPLQRKLDEFGRQLSHAISVICVAVWVINIGHFA
  DPAHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICS
  DKTGTLTTNQMSVCRMFVVAEAEAGTCRLHEFTISGTTYTPEGEVRQGEQPVRCGQFDGLVELATICALC
  NDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLKGLSRVERAGACNSVIKQLMRKEFTLEFSRD
  RKSMSVYCTPTRADPKVQGSKMFVKGAPESVIERCSSVRVGSRTAPLSTTSREHILAKIRDWGSGSDTLR
  CLALATRDTPPRKEDMHLDDCSRFVQYETDLTFVGCVGMLDPPRPEVAACITRCSRAGIRVVMITGDNKG
  TAVAICRRLGIFGDTEDVLGKAYTGREFDDLSPEQQRQACRTARCFARVEPAHKSRIVENLQSFNEITAM
  TGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYNNMKQFIRYLISSNVG
  EVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEKPPRNPREALISGWLFFRYLAI
  GVYVGLATVAAATWWFLYDTEGPQVTFYQLRNFLKCSEDNPLFAGIDCKVFESRFPTTMALSVLVTIEMC
  NALNSVSENQSLLRMPPWLNPWLLGAVVMSMALHFLILLVPPLPLIFQVTPLSGRQWGVVLQMSLPVILL
  DEALKYLSRNHMDEKKDLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163336
• ORF Size: 2997 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163336.1, NP_001156808.1
• RefSeq Size: 4520 bp
• RefSeq ORF: 3000 bp
• Locus ID: 53313
• UniProt ID: Q64518
• Cytogenetics: 11 B4
• MW: 110 kDa
• Gene Summary: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.[UniProtKB/Swiss-Prot Function]