Pcdha7 (BC080863) Mouse Tagged ORF Clone

CAT#: MR211274

Pcdha7 (Myc-DDK-tagged) - Mouse protocadherin alpha 7 (cDNA clone MGC:90581 IMAGE:30356469)

Product Images

Circular map for MR211274

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pcdha7
• Synonyms: Cnr4; Crnr4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211274 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAAACCTGAGAGGATACAACTGGAAGAGCCAACAATTACTCCTGTTCCTGATAATTGTGGCAGCCT
  GGGAGGCAGGGAGCGGCCAGCTCCACTACTCCGTCCCAGAGGAAGCCAAACACGGCACCTTCGTGGGCCG
  CATCGCGCAGGACCTGGGGCTGGAGCTGACGGAGCTGGTGCCCCGCCTGTTCAGGGTGGCGTCCAAGGAC
  CGCGGGGACCTTCTGGAGGTAAATCTTCAGAATGGCATTTTGTTTGTGAATTCTCGCATCGACCGGGAGG
  AACTGTGCGGGCGGAGCGCGGAGTGTAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAGGTTTT
  CCACGTGGAGGTGGAGGTGAAGGACATTAATGACAACCCTCCCATGTTTCCAGCGACACAAAAGGCTCTG
  TTTATCCTGGAGTCCAGACTGCTTGACTCGCGGTTTCCACTAGAGGGCGCGTCAGATGCAGATGTCGGTT
  CCAATGCTCTGCTGACTTACAGACTGAGCACCAATGAACATTTCTCTCTGGATGTACCGCCCAACCACGA
  GCAGGTGAAACCTCTTGGGCTGGTTTTGCGGAAACCTTTAGACAGAGAAGAAGCTGCAGAGATTCGCTTA
  TTGCTCACGGCCACCGACGGAGGCAAACCGGAGCTGACAGGCACCGTTCAATTACTCATCACCGTGCTGG
  ATGTCAATGACAATGCTCCAGTTTTTGACAGATCTCTGTATACTGTGAAATTGCCAGAAAACGTTCCAAA
  TGGAACATTGGTAATCAAAGTCAATGCCTCAGATTTGGACGAAGGCGTGAATGGGGACGTTATGTACTCA
  TTTTCTAGTGATGTTTCTTCAGATATAAAATCCAAGTTCCACATGGACACTGTGAGTGGAGAGATTACAG
  TTATAGGAATTATTGATTTCGAAGAGAGTAAAGCTTACAAGATTCCATTGGAGGCACGTGATAAAGGGTT
  CCCACAACTGCCTGGACACTGTACCATTCTTGTGGAAGTTGTAGATGCTAATGACAACGCTCCACAGTTG
  ACGGTTAGTTCCTTGTCTCTCCCAGTATCCGAGGACAGTCAACCTGGAAGAGTCGTCACTTTAATTAGCG
  TATTTGACCGTGATTCCGGTGCCAATGGGCAGGTGACCTGCTCCCTGACTCCTCATATCCCCTTCAAGCT
  GGTGTCCACATTCAAAAATTACTATTCGCTCGTGCTGGACAGTGCTCTGGACCGAGAGACCATCGCTAAC
  TATGATGTGATTGTGACCGCTCGCGATGGCGGTTCGCCCTCCCTGTGGGCCACGGCCAGCGTGTCCGTGG
  AGGTGGCTGATGTGAACGACAATGCTCCACTGTTTGCGCAGCCTGAATACACGGTGTTCGTGAAGGAGAA
  CAACCCGCCTGGTGCGCACATCTTTACAGTGTCAGCGATGGATGCTGATGCGCAGGAGAATGCGCTGGTG
  TCCTACTCGCTGGTGGAACGGAGGGTGGGCGAGCGCTTGCTGTCGAGCTATGTGTCTGTGCACGCGGAGA
  GCGGCAAGGTGTTCGCTCTGCAGCCTCTGGACCATGAGGAGCTGGAACTGCTGCAGTTCCAGGTGAGCGC
  GCGGGATGCTGGTGTGCCTGCCCTGGGCAGCAATGTGACTCTGCAGGTGTTTGTACTGGACGAGAACGAC
  AATGCACCTACCCTGCTGGGGCCTTTGGCTAACGGGGTTGGTGGCACAATGAATGAGGTAGTATCAAGAG
  CTTTGGTGCCTGGTCAGGTTGTGGCGAAAGTCCGGGCCGTAGATGAGGACTCTGGATACAATGCGTGGTT
  GTCTTTTGAGCTGCAGCCAGTTGCGGGTGGAGTTCGTAGTCCCTTCCGTGTAGGCCTGTACACAGGAGAA
  ATCAGCACGACACGTGCTCTGGAGGAGACAGATGCCTTGCGGCAGTGCTTGTTAGTGTTGGTGAAAGATC
  ACGGAGAACCTTCTCTGACCGCCACAGCCACGGTGTTGCTTTCTCTGGTAGACAGCGGCCAAACACAGAA
  GGTCTCATCTAAGGTGTCAGCTGGAGCCTCCAGAGTGGATCAGAGGCTGGTGGATGTCAACGTGTACCTG
  ATCATCGCCATCTGCGCGGTGTCCAGCCTGTTGGTGCTCACGCTACTGCTGTACACAGCGCTGCGCTGTT
  CGGCGACGCCCACCGATGGAGCCTGTGCTCCAGGGAAGCCCATGCTGGTGTGCTCCAGCGCGGTGGGGAG
  CTGGTCATACTCGCAGCAGAGGCGACAGAGAGTGTGCTCTGGAGAAGGCCCACCCAAGACCGACCTCATG
  GCCTTCAGCCCCAGCCTACCTCAGGGTCCCAGCTCTACAGATAACCCCCGGCAGCCCAACCCTGACTGGC
  GCTACTCTGCCTCGCTAAGAGCAGGCATGCACAGCTCTGTGCACCTGGAGGAGGCTGGCATTCTACGGGC
  TGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTATCCAGTGCAACACCAGAACCTGAGGCAGGAGAG
  GTGTCCCCTCCGGTGGGCGCCGGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCA
  AACAGTCCGGTCCCGGTGAGTTGCCAGACAAATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCG
  GCAGGAGCCTGCTAACATCTCCATCCGGCAGGAGCCTGCTAACAACCAAATTGACAAAAGCGATTTTATA
  ACCTTCGGCAAAAAGGAGGAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAA
  AAGAGAAAGGGAACAGCACGACGGACAACAGTGACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211274 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVNLRGYNWKSQQLLLFLIIVAAWEAGSGQLHYSVPEEAKHGTFVGRIAQDLGLELTELVPRLFRVASKD
  RGDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVEVEVKDINDNPPMFPATQKAL
  FILESRLLDSRFPLEGASDADVGSNALLTYRLSTNEHFSLDVPPNHEQVKPLGLVLRKPLDREEAAEIRL
  LLTATDGGKPELTGTVQLLITVLDVNDNAPVFDRSLYTVKLPENVPNGTLVIKVNASDLDEGVNGDVMYS
  FSSDVSSDIKSKFHMDTVSGEITVIGIIDFEESKAYKIPLEARDKGFPQLPGHCTILVEVVDANDNAPQL
  TVSSLSLPVSEDSQPGRVVTLISVFDRDSGANGQVTCSLTPHIPFKLVSTFKNYYSLVLDSALDRETIAN
  YDVIVTARDGGSPSLWATASVSVEVADVNDNAPLFAQPEYTVFVKENNPPGAHIFTVSAMDADAQENALV
  SYSLVERRVGERLLSSYVSVHAESGKVFALQPLDHEELELLQFQVSARDAGVPALGSNVTLQVFVLDEND
  NAPTLLGPLANGVGGTMNEVVSRALVPGQVVAKVRAVDEDSGYNAWLSFELQPVAGGVRSPFRVGLYTGE
  ISTTRALEETDALRQCLLVLVKDHGEPSLTATATVLLSLVDSGQTQKVSSKVSAGASRVDQRLVDVNVYL
  IIAICAVSSLLVLTLLLYTALRCSATPTDGACAPGKPMLVCSSAVGSWSYSQQRRQRVCSGEGPPKTDLM
  AFSPSLPQGPSSTDNPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSSATPEPEAGE
  VSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPANISIRQEPANNQIDKSDFI
  TFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC080863
• ORF Size: 2838 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC080863, AAH80863
• RefSeq Size: 3882 bp
• RefSeq ORF: 2840 bp
• Locus ID: 12939
• Cytogenetics: 18 B2- B3
• MW: 102.3 kDa
• Gene Summary: Calcium-dependent cell-adhesion protein involved in cells self-recognition and non-self discrimination (PubMed:27161523). Thereby, it is involved in the establishment and maintenance of specific neuronal connections in the brain (PubMed:27161523).[UniProtKB/Swiss-Prot Function]