Rbm12b1 (NM_028226) Mouse Tagged ORF Clone

CAT#: MR210892

Rbm12b1 (Myc-DDK-tagged) - Mouse RNA binding motif protein 12B (Rbm12b)

Product Images

Circular map for MR210892

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rbm12b1
• Synonyms: 3000004N20Rik; Rbm12b; Rbm12ba
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210892 representing NM_028226
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTAGTCATCCGTTTACTGGGGCTTCCTTTTATTGCGGGTCCTGTGGATATCCGTCACTTCTTCA
  AGGGATTGACTATTCCTGATGGAGGAGTGCATATAATTGGAGGGAAAGTTGGGGAGGCTTTTATAATTTT
  TGCAACAGATGAAGATGCAAGACGTGCTATAAGTCGCTCAGGAGGGTTTATCAAGGATTCATCTGTAGAG
  CTTTTTCTTAGTAGCAAGGTAGAAATGCAGAAGACCATAGAAATGAAAAGAACTGCTCGAGTAGGAAGAG
  GGCGACCAGGATCCGGGGCATCAGGGGTTGGCAACGTGTATCATTTTAGTGATGCTCTGAAGGAAGAAGA
  AAGTTATTCTGGATATGGCTCTTCGGTTAATCGAGATGCTGGGTTTCATACAAATGGTACAGGACTTGAC
  TTAAGGCCAAGAAAGACCAGGCCATTGAAGGCTGAGAATCCTTACTTATTTCTACGAGGTTTGCCTTACT
  TAGTAAATGAAGATGATGTCCGTGTCTTTTTCTCTGGTTTGTGTGTAGATGGAGTAATTCTCTTAAAACA
  TCATGATGGCCGAAATAATGGTGATGCTATAGTAAAATTTGCTTCATGTGTTGATGCTTCAGGAGGTCTT
  AAATGCCATAGAAGTTTCATGGGTTCAAGATTTATAGAAGTCATGCAGGGTTCAGAACAACAGTGGATTG
  AATTTGGTGGTACTGCAACCGAGGGCGGTGACACTCCTCGTATGAGATCTGAAGAGCATTCTCCTTCAAG
  AAGGATTAATGGTAGACATTTTCGGAAACGGTCTCATTCAAAATCTCCTAGAGCACGTTCTCGCTCTCCT
  CTTGGATTTTATGTTCACTTAAAAAATCTGTCCTTAAATACTAACAAAAGAGATTTAAGGAATCTCTTTA
  GAGATACTGACCTGACTAATGACCAGATTAAGTTTGTGTATAAAGATGAAAGAAGAACACGCTATGCCTT
  TGTGATGTTCAAGAATCAAAAAGACTATAATACTGCCCTGGGTTTGCATAAGACTGTTTTACAATATCGT
  CCAGTCCTTATTGATCCAGTGTCTAGGAAGGAAATGGTGAGAATCATTGAATGCTATGAGAAGAAGAGAC
  CAGAGTCTTTAGAGAAGGAAAGGCCAGGACGTGTTTCACAAAAGTACTCTCAAGAAGGCTTCTCTGGCTC
  TGGTCAGAAGCTGTGCATATATATAAGAAATTTACCATTTGATGTTACAAAGGGTGAAGTGCAGAAGTTC
  TTTGCAGACTTTTCTCTTGTTGAGGATGACATTTATCTGCTTTGTGATGACAAGGGAGTTGGTTTGGGAG
  AAGCACTGGTGAGATTTAAATCAGAAGAACAAGCCATGAAAGCTGAACGTTTGAACCGACAAAGATTCTT
  GGGGATAGAGGTGTTGTTAAGACTTATATCTGAGGAACAAATGCAGGAGTTTGGTGTAAAGTCTTCCTGG
  TTGTCTAATGAGAGGACACAAGCGTGTTCCAGGTCACACGATGGAGATGACTGTTCCTGTTTGTTTGACT
  TAAAAGATCCGTCATCCTGTTCATTTGGCCAGTCTGAAAGCCTTCGTTACCATCCAAAAGACTTAAGGAA
  AATGGGTCATTTCAAGCATCCCCAAGGGTATTTCCGGCAGTCTGACAGGCGCTCTCCTGAGGACTTCAGG
  CACTCCCCAGAAGACTATAGGCACCCCTGGGAGGAGCATACCAGCCACTCTAGGGAGGAAGACTGGAGAC
  TGCCTCTGGAGGATTGGAAATGGTCACAGGAAGATGATTTTAGGCAGTGTCATGAGAAAGATCGTAGGCA
  GCTAAGAAGCCCTTGGGAAGAAGACTTCAGACGGCCATCTCAGGAGCACTTCAGGAGATCCTATCAGGAG
  CACATTAGACAGCCACCCGAAGAACATTTCAGGCGTTCCCGAGAGGAAGATTTCAGGCATGTGGCTGATG
  AAGACTTTAGGCAAGCTTCTGATGAGGACTTCCGGACCTCCCAAGAAGATTTGAGATATCCCACTGATGA
  GGACTTTAGGAGGGTGTCTGTGGAAGACCTCAGGGAAGTTCCAGAGAAGGACCTTCGACTTCCTAAAAAT
  TTTAGATCTCCTGGAGAGGAGTTTTGGACCCCACCTGATTTCAGAGGTCAACATCCCTTTGGGAACTTTG
  ATCATCTACAAGGTGGCAAATTTGATTTTGAAAAGTATAAATTTGGAAGTTTTCCTGATGCAAAAGTTAC
  CTCTGATCTAAATTTAAATTGTGTTTCAGATAAAATAATTCCTGTTAAGATATCCAATCTTCCATTCAAA
  GCTAATGCTAGTGAAATTCTAGACTTTTTTCATGGTTATAAAGTCATACCTGATTCCGTTTCATTACAGT
  ATAATGAGCAAGGATTACCTATAGGAGAAGCCATTGTTGCTATGACAAATTATAATGAAGCTCTAGCTGC
  AGTTAAAGATCTAAGTGGTAGACCAGTTGGTCCCCGCAAAGTTAAGCTTAGTTTGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210892 representing NM_028226
  Red=Cloning site Green=Tags(s)
  
  MAVVIRLLGLPFIAGPVDIRHFFKGLTIPDGGVHIIGGKVGEAFIIFATDEDARRAISRSGGFIKDSSVE
  LFLSSKVEMQKTIEMKRTARVGRGRPGSGASGVGNVYHFSDALKEEESYSGYGSSVNRDAGFHTNGTGLD
  LRPRKTRPLKAENPYLFLRGLPYLVNEDDVRVFFSGLCVDGVILLKHHDGRNNGDAIVKFASCVDASGGL
  KCHRSFMGSRFIEVMQGSEQQWIEFGGTATEGGDTPRMRSEEHSPSRRINGRHFRKRSHSKSPRARSRSP
  LGFYVHLKNLSLNTNKRDLRNLFRDTDLTNDQIKFVYKDERRTRYAFVMFKNQKDYNTALGLHKTVLQYR
  PVLIDPVSRKEMVRIIECYEKKRPESLEKERPGRVSQKYSQEGFSGSGQKLCIYIRNLPFDVTKGEVQKF
  FADFSLVEDDIYLLCDDKGVGLGEALVRFKSEEQAMKAERLNRQRFLGIEVLLRLISEEQMQEFGVKSSW
  LSNERTQACSRSHDGDDCSCLFDLKDPSSCSFGQSESLRYHPKDLRKMGHFKHPQGYFRQSDRRSPEDFR
  HSPEDYRHPWEEHTSHSREEDWRLPLEDWKWSQEDDFRQCHEKDRRQLRSPWEEDFRRPSQEHFRRSYQE
  HIRQPPEEHFRRSREEDFRHVADEDFRQASDEDFRTSQEDLRYPTDEDFRRVSVEDLREVPEKDLRLPKN
  FRSPGEEFWTPPDFRGQHPFGNFDHLQGGKFDFEKYKFGSFPDAKVTSDLNLNCVSDKIIPVKISNLPFK
  ANASEILDFFHGYKVIPDSVSLQYNEQGLPIGEAIVAMTNYNEALAAVKDLSGRPVGPRKVKLSLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028226
• ORF Size: 2508 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028226.2, NP_082502.2
• RefSeq Size: 3313 bp
• RefSeq ORF: 2511 bp
• Locus ID: 72397
• UniProt ID: Q80YR9
• Cytogenetics: 4 A1
• MW: 97 kDa