Ppp4r3b (NM_134034) Mouse Tagged ORF Clone

CAT#: MR210812

Smek2 (Myc-DDK-tagged) - Mouse SMEK homolog 2, suppressor of mek1 (Dictyostelium) (Smek2)

Product Images

Circular map for MR210812

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp4r3b
• Synonyms: AW011752; AW557776; mKIAA1387; Smek2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210812 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGATACACGGAGGCGAGTGAAGGTCTATACCCTTAACGAAGACCGGCAATGGGACGACCGAGGCA
  CCGGGCACGTCTCCTCCACTTACGTAGAGGAGCTCAAGGGGATGTCGCTGCTGGTTCGTGCCGAGTCCGA
  CGGATCGCTACTCTTAGAGTCAAAGATAAATCCAAACACTGCATATCAGAAACAGCAGGATACATTAATT
  GTTTGGTCAGAAGCTGAAAACTATGATCTGGCTCTAAGTTTTCAAGAAAAAGCTGGCTGTGATGAAATTT
  GGGAAAAAATTTGTCAAGTTCAAGGTAAGGACCCATCAGTAGAAGTCACACAGGACCTCATTGATGAATC
  TGAAGAAGAACGATTTGAAGAAATGCCAGAAACTAGTCATCTAATTGACCTGCCCACATGTGAACTCAGT
  AAACTTGAAGAGATTGCTGACTTAGTTACCTCAGTTCTCTCCTCACCTATCCGTAGGGAAAAGCTGGCTC
  TGGCCTTGGAAAATGAAGGCTATATCAAAAAACTACTACAGCTATTCCAAGCTTGTGAGAACCTAGAAAA
  CACTGAAGGCTTACACCATCTGTATGAAATTATTAGAGGAATCTTATTCCTAAATAAGGCAACACTTTTT
  GAGGTAATGTTTTCTGATGAGTGTATCATGGATGTCGTGGGATGCCTTGAATATGATCCTGCTTTGGCTC
  AGCCAAAAAGACATAGAGAATTCTTAACTAAAACTGCAAAGTTTAAGGAAGTTATACCAATAACAGATTC
  GGAACTAAGGCAAAAAATACATCAGACTTACAGGGTACAGTACATTCAGGACATCATTTTGCCTACACCA
  TCTGTTTTTGAAGAAAATTTCCTTTCTACTCTTACATCTTTTATTTTCTTCAACAAAGTTGAAATAGTCA
  GCATGCTGCAGGAAGATGAGAAGTTTTTGTCTGAAGTTTTTGCACAATTAACAGATGAAGCTACAGATGA
  TGATAAACGGCGCGAGTTGGTTAATTTCTTCAAAGAATTTTGTGCATTTTCTCAGACATTGCAACCTCAG
  AACAGGGATGCTTTTTTCAAAACCTTAGCAAAATTGGGAATTCTTCCAGCTCTTGAAATTGTAATGGGCG
  TGGACGACTTGCAAGTCAGATCAGCTGCTACAGATATATTCTCTTATCTGGTGGAGTTTAGTCCATCCAT
  GGTCCGAGAGTTTGTGATGCAGGAAGCCCAGCAGAGTGATGATGATGTACTTCTTATTAATGTGGTGATT
  GAACAGATGATTTGTGATACTGACCCTGAGCTCGGAGGTGCTGTTCAGTTAATGGGACTTCTTCGTACTC
  TAATTGATCCAGAGAACATGTTGGCTACAACTAATAAAACTGAAAAGAGTGAGTTCTTAAATTTCTTCTA
  CAACCATTGTATGCATGTTCTCACTGCTCCACTTTTGACCAATACGTCAGAAGACAAATGCGAGAAAGAT
  AATATGCTTGGATCTAACACAACCAACACAATTTGCCCTGATAATTATCAAACAGCACAGCTGCTTGCCT
  TAATTTTAGAGTTACTCACATTTTGTGTGGAACATCACACATACCACATAAAAAACTACATCATGAACAA
  GGACTTGCTAAGAAGAGTCTTGGTGTTGATGAATTCAAAGCACACTTTTCTGGCCTTGTGTGCTCTTCGC
  TTTATGAGGCGAATAATTGGCCTGAAAGATGAATTTTATAATCGTTACATCACCAAAGGAAATCTTTTTG
  AGCCAGTTATAAATGCCCTTTTGGATAATGGAACTCGGTACAATCTTTTAAATTCAGCTGTTATTGAATT
  GTTTGAATTTATAAGAGTGGAAGATATCAAGTCTCTTACTGCACATATAGTTGAAAACTTTTATAAAGCA
  CTTGAATCGATTGAATATGTTCAGACATTCAAAGGATTGAAGACTAAATATGAACAAGAAAAAGACAGAC
  AAAATCAGAAACTGAACAGTGTACCATCTATATTGCGTAGTAACCGATTTCGCAGAGATGCAAAAGCCTT
  AGAAGAGGATGAAGAAATGTGGTTTAATGAAGATGATGATGAAGAAGGAAAGGCAGTCATAACACCAGTT
  GAAAAATCTAAGACTGAAGATGATTTTCCAGATAGTTATGAAAAATTTATGGAAACTAAAAAAGCAAAAG
  AAAGTGAAGACAAAGAAAACCTTCCCAAAAGGGCATCATCTGGTGGCTTCAAATTTACTTTCTCCCATTC
  CCCCAGTGCTACTAATGGAACAAACAGTACAAACAGTAAGTCTGTAGTGAGTCAGACAACACCAGCAAGT
  TCTAATGTGGCCTCTTCCAAAACTACAAGCCTGGCTACATCAGTGACAGCCACAAAGGGAAATTTGGTTG
  GCTTGGTAGACTATCCAGATGATGAAGAGGAAGATGAGGAAGAAGAATCATCCCCTAGGAAAAGACCTCG
  CCTGGGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210812 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSDTRRRVKVYTLNEDRQWDDRGTGHVSSTYVEELKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLI
  VWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVEVTQDLIDESEEERFEEMPETSHLIDLPTCELS
  KLEEIADLVTSVLSSPIRREKLALALENEGYIKKLLQLFQACENLENTEGLHHLYEIIRGILFLNKATLF
  EVMFSDECIMDVVGCLEYDPALAQPKRHREFLTKTAKFKEVIPITDSELRQKIHQTYRVQYIQDIILPTP
  SVFEENFLSTLTSFIFFNKVEIVSMLQEDEKFLSEVFAQLTDEATDDDKRRELVNFFKEFCAFSQTLQPQ
  NRDAFFKTLAKLGILPALEIVMGVDDLQVRSAATDIFSYLVEFSPSMVREFVMQEAQQSDDDVLLINVVI
  EQMICDTDPELGGAVQLMGLLRTLIDPENMLATTNKTEKSEFLNFFYNHCMHVLTAPLLTNTSEDKCEKD
  NMLGSNTTNTICPDNYQTAQLLALILELLTFCVEHHTYHIKNYIMNKDLLRRVLVLMNSKHTFLALCALR
  FMRRIIGLKDEFYNRYITKGNLFEPVINALLDNGTRYNLLNSAVIELFEFIRVEDIKSLTAHIVENFYKA
  LESIEYVQTFKGLKTKYEQEKDRQNQKLNSVPSILRSNRFRRDAKALEEDEEMWFNEDDDEEGKAVITPV
  EKSKTEDDFPDSYEKFMETKKAKESEDKENLPKRASSGGFKFTFSHSPSATNGTNSTNSKSVVSQTTPAS
  SNVASSKTTSLATSVTATKGNLVGLVDYPDDEEEDEEEESSPRKRPRLGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134034
• ORF Size: 2463 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134034.1, NP_598795.1
• RefSeq Size: 5111 bp
• RefSeq ORF: 2463 bp
• Locus ID: 104570
• UniProt ID: Q922R5
• Cytogenetics: 11 A3.3
• MW: 93.9 kDa
• Gene Summary: Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers (By similarity).[UniProtKB/Swiss-Prot Function]