Ece1 (NM_199307) Mouse Tagged ORF Clone

CAT#: MR210476

Ece1 (Myc-DDK-tagged) - Mouse endothelin converting enzyme 1 (Ece1)

Product Images

Circular map for MR210476

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ece1
• Synonyms: AW322500; ECE-1; ECE-1a; ECE-1b; ECE-1d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210476 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAACCTACAAGCGGGCCACGCTGGACGAAGAGGATCTGGTGGACTCACTCTCCGAGGGCGATGTGT
  ACCCCAATGGCCTACAGGTGAACTTCCGCAGCTCCCGGAGCGGCCAGAGGTGCTGGGCAGCTCGGACCTC
  GGTGGAGAAGCGGCTGGTGGTGCTGGTGACGCTTCTGGCGGCAGGGTTGGTGGCCTGCCTGGCAGCCCTG
  GGCATCCAGTACCAGACAAGAACGCCTCCGGTATGTCTGACGGAGGCCTGTGTCTCAGTGACCAGCTCTA
  TCCTAAACTCCATGGACCCCACAGTAGACCCGTGCCAGGACTTCTTCAGCTACGCCTGCGGGGGCTGGAT
  CAAGGCCAACCCCGTGCCCGATGGCCACTCACGCTGGGGAACCTTCAGCAACCTCTGGGAACACAACCAA
  GCTGTCATCAAGCATCTACTGGAAAATGCCACGGCCAGTGTGAGCGAGGCAGAGAGAAAAGCCCAAGTGT
  ACTACCGTGCGTGCATGAACGAAACTAGGATTGAGGAGCTTCGGGCCAAGCCCCTGATGGAGCTGATTGA
  GAAGCTGGGAGGCTGGAATATCACAGGACCCTGGGCCAAGGACAACTTCCAGGACACACTGCAGGTGGTC
  ACAGCTCACTACCGCACCTCACCCTTCTTCTCTGTTTATGTCAGCGCGGACTCCAAGAATTCCAACAGCA
  ATGTGATCCAGGTGGACCAGTCCGGCCTTGGCTTGCCCTCCAGAGACTATTACCTGAACAAGACAGAGAA
  TGAAAAGGTCCTGACTGGATACCTGAACTACATGGTCCAGCTGGGGAAGCTCCTGGGCGGTGGGGACGAG
  GATGCCATCCGGCCACAGATGCAGCAGATCCTGGATTTTGAGACGGCGCTGGCCAACATCACCATCCCCC
  AGGAGAAGCGCAGGGATGAAGAACTCATCTACCACAAAGTCACGGCTGCCGAGCTGCAGACCTTGGCACC
  TGCCATCAACTGGTTACCCTTTCTGAACACCATTTTTTACCCAGTGGAGATCAATGAGTCTGAGCCAATC
  GTGGTCTACGACAAGGAATACTTGAGACAAGTCTCCACCCTCATCAACAACACCGACAAATGTCTGCTCA
  ACAACTACATGATGTGGAACCTGGTACGCAAAACAAGCTCCTTCCTGGACCAGCGCTTTCAGGACGCCGA
  CGAGAAGTTCATGGAGGTTATGTATGGGACGAAGAAGACCTGTATTCCCCGCTGGAAGTTTTGTGTGAGT
  GACACGGAAAACAACCTGGGCTTTGCCCTGGGACCCATGTTTGTGAAAGCAACCTTTGCAGAAGACAGTA
  AGAATATAGCCAGTGAGATCATCATGGAGATCAAGAAGGCGTTTGAGGAGAGTCTGAGCACTCTGAAATG
  GATGGATGAAGAGACTCGGAGATCAGCCAAGGAGAAGGCGGATGCTATCTACAACATGATAGGCTACCCC
  AACTTCATCATGGACCCCAAGGAGCTGGACAAAGTCTTCAATGACTACACAGCAGTTCCTGATCTCTACT
  TTGAGAACGCCATGCGCTTTTTCAACTTCTCATGGAGGGTCACAGCTGACCAGCTCAGAAAAGCTCCCAA
  CAGAGATCAGTGGAGTATGACGCCGCCCATGGTGAACGCTTACTACTCGCCCACCAAGAACGAGATCGTG
  TTTCCAGCTGGAATACTGCAGGCGCCATTTTATACCCGCTCTTCACCCAACGCCTTGAACTTCGGTGGCA
  TTGGTGTCGTAGTGGGCCACGAGCTGACTCACGCTTTCGATGATCAAGGTCGGGAGTACGACAAGGATGG
  GAACCTTCGACCCTGGTGGAAGAACTCGTCCGTGGAGGCGTTCAAGCAGCAGACGGAGTGCATGGTGCAG
  CAATACAGCAACTACAGCGTGAACGGGGAGCCCGTGAACGGTCGACACACCCTCGGGGAGAACATTGCCG
  ACAACGGGGGACTCAAGGCGGCCTACCGGGCGTACCAGAACTGGGTAAAGAAGAACGGAGCTGAGCAGAC
  GCTGCCCACCCTGGGTCTCACCAGCAACCAGCTCTTCTTCCTAGGATTTGCACAGGTCTGGTGCTCTGTC
  CGCACACCTGAGAGCTCCCACGAAGGCCTCATCACGGATCCGCACAGCCCCTCTCGCTTCCGGGTCATCG
  GCTCGCTCTCCAACTCCAAGGAGTTCTCAGAACACTTTCGCTGCCCGCCTGGCTCTCCCATGAACCCTCA
  TCACAAGTGCGAAGTCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210476 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTYKRATLDEEDLVDSLSEGDVYPNGLQVNFRSSRSGQRCWAARTSVEKRLVVLVTLLAAGLVACLAAL
  GIQYQTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQ
  AVIKHLLENATASVSEAERKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVV
  TAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDE
  DAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPI
  VVYDKEYLRQVSTLINNTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCIPRWKFCVS
  DTENNLGFALGPMFVKATFAEDSKNIASEIIMEIKKAFEESLSTLKWMDEETRRSAKEKADAIYNMIGYP
  NFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIV
  FPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQ
  QYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTSNQLFFLGFAQVWCSV
  RTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPHHKCEVW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199307
• ORF Size: 2262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199307.2, NP_955011.1
• RefSeq Size: 4856 bp
• RefSeq ORF: 2262 bp
• Locus ID: 230857
• UniProt ID: Q4PZA2
• Cytogenetics: 4 D3
• MW: 85.5 kDa
• Gene Summary: Converts big endothelin-1 to endothelin-1.[UniProtKB/Swiss-Prot Function]