Ece1 (NM_199307) Mouse Untagged Clone

CAT#: MC221329

Ece1 (untagged) - Mouse endothelin converting enzyme 1 (Ece1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ece1
• Synonyms: AW322500; ECE-1; ECE-1a; ECE-1b; ECE-1d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221329 representing NM_199307
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAACCTACAAGCGGGCCACGCTGGACGAAGAGGATCTGGTGGACTCACTCTCCGAGGGCGATGTGT
  ACCCCAATGGCCTACAGGTGAACTTCCGCAGCTCCCGGAGCGGCCAGAGGTGCTGGGCAGCTCGGACCTC
  GGTGGAGAAGCGGCTGGTGGTGCTGGTGACGCTTCTGGCGGCAGGGTTGGTGGCCTGCCTGGCAGCCCTG
  GGCATCCAGTACCAGACAAGAACGCCTCCGGTATGTCTGACGGAGGCCTGTGTCTCAGTGACCAGCTCTA
  TCCTAAACTCCATGGACCCCACAGTAGACCCGTGCCAGGACTTCTTCAGCTACGCCTGCGGGGGCTGGAT
  CAAGGCCAACCCCGTGCCCGATGGCCACTCACGCTGGGGAACCTTCAGCAACCTCTGGGAACACAACCAA
  GCTGTCATCAAGCATCTACTGGAAAATGCCACGGCCAGTGTGAGCGAGGCAGAGAGAAAAGCCCAAGTGT
  ACTACCGTGCGTGCATGAACGAAACTAGGATTGAGGAGCTTCGGGCCAAGCCCCTGATGGAGCTGATTGA
  GAAGCTGGGAGGCTGGAATATCACAGGACCCTGGGCCAAGGACAACTTCCAGGACACACTGCAGGTGGTC
  ACAGCTCACTACCGCACCTCACCCTTCTTCTCTGTTTATGTCAGCGCGGACTCCAAGAATTCCAACAGCA
  ATGTGATCCAGGTGGACCAGTCCGGCCTTGGCTTGCCCTCCAGAGACTATTACCTGAACAAGACAGAGAA
  TGAAAAGGTCCTGACTGGATACCTGAACTACATGGTCCAGCTGGGGAAGCTCCTGGGCGGTGGGGACGAG
  GATGCCATCCGGCCACAGATGCAGCAGATCCTGGATTTTGAGACGGCGCTGGCCAACATCACCATCCCCC
  AGGAGAAGCGCAGGGATGAAGAACTCATCTACCACAAAGTCACGGCTGCCGAGCTGCAGACCTTGGCACC
  TGCCATCAACTGGTTACCCTTTCTGAACACCATTTTTTACCCAGTGGAGATCAATGAGTCTGAGCCAATC
  GTGGTCTACGACAAGGAATACTTGAGACAAGTCTCCACCCTCATCAACAACACCGACAAATGTCTGCTCA
  ACAACTACATGATGTGGAACCTGGTACGCAAAACAAGCTCCTTCCTGGACCAGCGCTTTCAGGACGCCGA
  CGAGAAGTTCATGGAGGTTATGTATGGGACGAAGAAGACCTGTATTCCCCGCTGGAAGTTTTGTGTGAGT
  GACACGGAAAACAACCTGGGCTTTGCCCTGGGACCCATGTTTGTGAAAGCAACCTTTGCAGAAGACAGTA
  AGAATATAGCCAGTGAGATCATCATGGAGATCAAGAAGGCGTTTGAGGAGAGTCTGAGCACTCTGAAATG
  GATGGATGAAGAGACTCGGAGATCAGCCAAGGAGAAGGCGGATGCTATCTACAACATGATAGGCTACCCC
  AACTTCATCATGGACCCCAAGGAGCTGGACAAAGTCTTCAATGACTACACAGCAGTTCCTGATCTCTACT
  TTGAGAACGCCATGCGCTTTTTCAACTTCTCATGGAGGGTCACAGCTGACCAGCTCAGAAAAGCTCCCAA
  CAGAGATCAGTGGAGTATGACGCCGCCCATGGTGAACGCTTACTACTCGCCCACCAAGAACGAGATCGTG
  TTTCCAGCTGGAATACTGCAGGCGCCATTTTATACCCGCTCTTCACCCAACGCCTTGAACTTCGGTGGCA
  TTGGTGTCGTAGTGGGCCACGAGCTGACTCACGCTTTCGATGATCAAGGTCGGGAGTACGACAAGGATGG
  GAACCTTCGACCCTGGTGGAAGAACTCGTCCGTGGAGGCGTTCAAGCAGCAGACGGAGTGCATGGTGCAG
  CAATACAGCAACTACAGCGTGAACGGGGAGCCCGTGAACGGTCGACACACCCTCGGGGAGAACATTGCCG
  ACAACGGGGGACTCAAGGCGGCCTACCGGGCGTACCAGAACTGGGTAAAGAAGAACGGAGCTGAGCAGAC
  GCTGCCCACCCTGGGTCTCACCAGCAACCAGCTCTTCTTCCTAGGATTTGCACAGGTCTGGTGCTCTGTC
  CGCACACCTGAGAGCTCCCACGAAGGCCTCATCACGGATCCGCACAGCCCCTCTCGCTTCCGGGTCATCG
  GCTCGCTCTCCAACTCCAAGGAGTTCTCAGAACACTTTCGCTGCCCGCCTGGCTCTCCCATGAACCCTCA
  TCACAAGTGCGAAGTCTGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_199307
• Insert Size: 2262 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199307.2, NP_955011.1
• RefSeq Size: 4856 bp
• RefSeq ORF: 2262 bp
• Locus ID: 230857
• UniProt ID: Q4PZA2
• Cytogenetics: 4 D3
• Gene Summary: Converts big endothelin-1 to endothelin-1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3), as well as variant 4, encodes isoform c.