Hsd17b4 (NM_008292) Mouse Tagged ORF Clone
CAT#: MR210361
- TrueORF®
Hsd17b4 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 4 (Hsd17b4)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_008292" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Hsd17b4 |
Synonyms | 17-beta-HSD; 17[b]-HSD; DBP; MFE-2; Mfp-2; MFP2; MPF-2; perMFE-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210361 representing NM_008292
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGCCGCTGAGGTTCGACGGGCGTGTGGTCTTGGTCACCGGCGCCGGGGGAGGATTGGGCCGAG CTTACGCCCTGGCGTTTGCAGAAAGAGGAGCATTAGTCATTGTGAACGACTTAGGAGGGGACTTCAAGGG AATTGGTAAAGGCTCCTCTGCTGCAGACAAGGTTGTGGCAGAGATAAGAAGGAAAGGCGGAAAAGCAGTG GCCAATTACGATTCAGTTGAAGCAGGCGAGAAGCTTGTGAAGACGGCACTGGACACATTTGGCAGAATAG ACGTTGTGGTCAACAATGCTGGAATCCTGAGGGACCGTTCCTTCTCCAGGATAAGTGATGAAGACTGGGA TATAATTCATAGAGTTCATTTGCGGGGCTCCTTCCAAGTGACCCGGGCAGCATGGGACCATATGAAGAAA CAGAATTATGGAAGAATCCTTATGACTTCCTCAGCTTCTGGAATATATGGCAACTTTGGCCAGGCGAATT ATAGTGCTGCAAAGCTGGGCATTCTGGGTCTCTGCAATACTCTCGCCATTGAAGGCAGGAAGAACAACAT TCATTGCAACACCATTGCCCCCAACGCTGGGTCACGGATGACGGAGACTGTGTTGCCGGAAGATCTTGTT GAAGCCCTGAAGCCAGAGTATGTGGCCCCTCTGGTGCTTTGGCTTTGCCATGAGAGCTGTGAGGAAAATG GTGGCCTATTTGAGGTTGGAGCAGGATGGATTGGAAAATTGCGCTGGGAGAGGACCCTGGGCGCCATCGT CAGAAAGCGGAATCAGCCCATGACTCCCGAGGCAGTGAGGGACAACTGGGAGAAGATCTGTGACTTCAGC AATGCCAGCAAGCCGCAGACCATTCAAGAATCAACAGGTGGTATAGTCGAAGTTTTACATAAGGTAGATT CAGAAGGAATCTCACCAAACCGTACCAGTCACGCGGCACCTGCAGCCACGTCAGGATTCGTTGGTGCTGT TGGCCATAAACTTCCTTCATTTTCTTCTTCGTATACGGAGCTGCAGAGTATTATGTATGCCCTCGGAGTG GGAGCGTCAGTCAAAAATCCAAAGGATTTGAAGTTTGTTTATGAAGGCAGTGCTGACTTCTCCTGTTTGC CCACCTTCGGAGTCATTGTCGCTCAGAAGTCCATGATGAATGGAGGGCTGGCAGAGGTTCCTGGGCTGTC ATTCAACTTTGCAAAGGCTCTTCACGGGGAGCAGTACTTGGAGCTGTATAAGCCACTTCCTCGATCAGGA GAATTAAAATGTGAAGCAGTTATTGCTGACATCCTGGATAAAGGCTCTGGCGTAGTGATTGTTATGGACG TCTATTCTTATTCTGGGAAGGAACTTATATGCTATAATCAGTTCTCTGTCTTTGTTGTTGGCTCTGGGGG CTTTGGTGGAAAACGGACATCAGAAAAACTCAAAGCAGCTGTAGCTGTACCAAATCGACCTCCAGATGCT GTACTGAGAGATGCCACCTCACTGAATCAGGCCGCGCTGTACCGCCTCAGCGGAGACTGGAATCCTCTAC ACATTGACCCGGACTTTGCGAGCGTTGCCGGTTTTGAGAAGCCCATATTACATGGACTATGTACCTTTGG ATTTTCTGCAAGGCATGTTTTACAGCAGTTTGCAGATAATGATGTATCAAGATTCAAGGCGATTAAGGTT CGTTTTGCCAAACCAGTGTATCCAGGACAGACTCTACAAACTGAGATGTGGAAGGAAGGAAACAGAATTC ATTTTCAAACCAAGGTCCACGAGACTGGAGATGTTGTCATTTCAAATGCGTACGTGGATCTCGTGCCTGC ATCTGGAGTTTCAACCCAGACACCTTCAGAGGGTGGAGAGCTCCAGAGTGCTCTTGTGTTTGGGGAGATA GGCCGCCGCCTCAAGAGTGTTGGCCGTGAGGTGGTAAAGAAAGCGAATGCTGTGTTTGAATGGCATATCA CGAAAGGTGGGACTGTTGCAGCCAAGTGGACCATTGACCTGAAGAGCGGCTCAGGGGAGGTGTACCAAGG CCCCGCAAAGGGCTCTGCTGATGTGACCATCATCATTTCCGATGAGGATTTTATGGAAGTGGTCTTCGGC AAGCTTGACCCACAGAAGGCCTTCTTCAGTGGCAGGCTGAAGGCCAGAGGGAACATCATGCTGAGCCAGA AACTACAGATGATTCTTAAAGACTATGCCAAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210361 representing NM_008292
Red=Cloning site Green=Tags(s) MASPLRFDGRVVLVTGAGGGLGRAYALAFAERGALVIVNDLGGDFKGIGKGSSAADKVVAEIRRKGGKAV ANYDSVEAGEKLVKTALDTFGRIDVVVNNAGILRDRSFSRISDEDWDIIHRVHLRGSFQVTRAAWDHMKK QNYGRILMTSSASGIYGNFGQANYSAAKLGILGLCNTLAIEGRKNNIHCNTIAPNAGSRMTETVLPEDLV EALKPEYVAPLVLWLCHESCEENGGLFEVGAGWIGKLRWERTLGAIVRKRNQPMTPEAVRDNWEKICDFS NASKPQTIQESTGGIVEVLHKVDSEGISPNRTSHAAPAATSGFVGAVGHKLPSFSSSYTELQSIMYALGV GASVKNPKDLKFVYEGSADFSCLPTFGVIVAQKSMMNGGLAEVPGLSFNFAKALHGEQYLELYKPLPRSG ELKCEAVIADILDKGSGVVIVMDVYSYSGKELICYNQFSVFVVGSGGFGGKRTSEKLKAAVAVPNRPPDA VLRDATSLNQAALYRLSGDWNPLHIDPDFASVAGFEKPILHGLCTFGFSARHVLQQFADNDVSRFKAIKV RFAKPVYPGQTLQTEMWKEGNRIHFQTKVHETGDVVISNAYVDLVPASGVSTQTPSEGGELQSALVFGEI GRRLKSVGREVVKKANAVFEWHITKGGTVAAKWTIDLKSGSGEVYQGPAKGSADVTIIISDEDFMEVVFG KLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008292 |
ORF Size | 2205 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008292.4, NP_032318.2 |
RefSeq Size | 2685 bp |
RefSeq ORF | 2208 bp |
Locus ID | 15488 |
UniProt ID | P51660 |
Cytogenetics | 18 D1 |
MW | 79.5 kDa |
Gene Summary | Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205700 | Hsd17b4 (untagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 4 (Hsd17b4), (10ug) |
USD 674.00 |
|
MG210361 | Hsd17b4 (tGFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 4 (Hsd17b4) |
USD 873.00 |
|
MR210361L3 | Lenti ORF clone of Hsd17b4 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 4 (Hsd17b4) |
USD 973.00 |
|
MR210361L4 | Lenti ORF clone of Hsd17b4 (mGFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 4 (Hsd17b4) |
USD 973.00 |
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