Dlg4 (NM_001109752) Mouse Tagged ORF Clone

CAT#: MR210275

Dlg4 (Myc-DDK-tagged) - Mouse discs, large homolog 4 (Drosophila) (Dlg4), transcript variant 2

Product Images

Circular map for MR210275

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dlg4
• Synonyms: Dlgh4; PSD-95; PSD95; SAP90; SAP90A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210275 representing NM_001109752
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGTCTCTGTATAGTGACAACCAAGAAATACCGCTACCAAGATGAAGACACGCCCCCTCTGGAAC
  ACAGCCCGGCCCACCTCCCCAACCAGGCCAATTCTCCCCCTGTGATTGTCAACACGGACACCCTAGAAGC
  CCCAGGATATGTGAACGGAACAGAGGGGGAGATGGAGTATGAGGAGATCACATTGGAAAGGGGTAACTCA
  GGTCTGGGCTTCAGCATCGCAGGTGGCACCGACAACCCACACATCGGTGACGACCCATCCATCTTTATCA
  CCAAGATCATTCCTGGTGGGGCTGCAGCCCAGGATGGCCGCCTCAGGGTCAACGACAGCATCCTGTTTGT
  CAATGAAGTGGATGTCCGGGAGGTGACCCATTCAGCTGCAGTGGAGGCCCTCAAAGAGGCGGGTTCCATC
  GTTCGCCTCTACGTCATGCGCCGGAAACCCCCAGCTGAGAAGATCATAGAGATCAAGCTTATCAAAGGGC
  CTAAAGGACTTGGCTTCAGCATCGCAGGGGGCGTTGGGAACCAGCACATCCCTGGAGATAATAGCATCTA
  CGTAACCAAGATCATCGAAGGAGGCGCTGCCCACAAGGATGGCAGGTTGCAGATCGGAGACAAGATCCTG
  GCGGTCAACAGTGTGGGGCTAGAGGATGTCATGCATGAGGACGCCGTGGCAGCCCTGAAGAACACATATG
  ACGTTGTGTACCTAAAGGTGGCCAAGCCCAGCAATGCCTACCTGAGTGACAGCTATGCTCCCCCAGACAT
  CACAACCTCATATTCTCAGCACCTGGACAATGAGATCAGTCATAGCAGCTACTTGGGCACCGACTACCCC
  ACAGCCATGACCCCCACTTCCCCTCGGCGCTACTCCCCCGTGGCCAAGGACCTGCTAGGGGAGGAAGATA
  TTCCCCGGGAACCAAGGCGGATCGTGATCCATCGGGGCTCCACCGGCCTGGGCTTCAACATTGTGGGCGG
  CGAGGACGGTGAAGGCATCTTCATCTCCTTCATCCTTGCTGGGGGTCCAGCTGACCTCAGTGGGGAGCTA
  CGGAAGGGGGACCAGATCCTGTCGGTCAATGGTGTTGACCTCCGCAATGCCAGTCATGAACAGGCTGCCA
  TTGCCCTGAAGAACGCGGGTCAGACGGTCACGATCATCGCTCAGTATAAACCAGAAGAGTATAGCCGATT
  CGAGGCCAAGATCCATGATCTTCGGGAACAGCTTATGAATAGTAGCCTGGGCTCAGGGACTGCATCTCTG
  CGAAGCAACCCCAAGCGGGGCTTCTATATCAGGGCCCTGTTTGACTACGACAAGACCAAGGACTGCGGTT
  TCTTGAGCCAGGCCCTGAGCTTCCACTTTGGGGATGTGCTTCATGTAATTGACGCCAGCGACGAAGAGTG
  GTGGCAAGCGCGGCGGGTCCACTCTGACAGTGAGACCGATGACATTGGCTTCATTCCCAGCAAACGGCGG
  GTCGAGCGACGAGAGTGGTCAAGGTTAAAGGCCAAGGACTGGGGCTCCAGCTCTGGATCACAGGGTCGAG
  AAGACTCGGTTCTGAGCTATGAGACGGTGACGCAGATGGAAGTGCACTACGCTCGCCCCATCATCATCCT
  TGGGCCTACCAAAGACCGTGCCAACGATGATCTTCTCTCCGAGTTCCCCGACAAGTTTGGATCCTGTGTC
  CCTCATACGACACGTCCTAAGCGGGAATATGAGATAGACGGCCGCGATTACCACTTTGTCTCCTCCCGGG
  AGAAAATGGAGAAGGACATTCAGGCGCACAAGTTCATTGAGGCTGGCCAGTACAACAGCCACCTCTACGG
  GACCAGCGTCCAGTCTGTGCGAGAGGTAGCAGAGCAGGGGAAGCACTGCATCCTTGATGTCTCAGCCAAT
  GCCGTGCGGCGGCTGCAGGCGGCCCACCTGCACCCTATCGCCATCTTCATCCGTCCCCGCTCCCTGGAGA
  ATGTGCTAGAGATCAATAAGCGGATCACAGAGGAGCAAGCCCGGAAAGCCTTCGACAGGGCCACGAAGCT
  GGAGCAGGAGTTCACGGAGTGCTTCTCAGCCATCGTAGAGGGCGACAGCTTTGAAGAGATCTATCACAAA
  GTGAAACGTGTCATCGAAGACCTCTCAGGCCCCTACATCTGGGTCCCAGCCCGAGAGAGACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210275 representing NM_001109752
  Red=Cloning site Green=Tags(s)
  
  MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYVNGTEGEMEYEEITLERGNS
  GLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSI
  VRLYVMRRKPPAEKIIEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKIL
  AVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYP
  TAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGEL
  RKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASL
  RSNPKRGFYIRALFDYDKTKDCGFLSQALSFHFGDVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRR
  VERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCV
  PHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSAN
  AVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHK
  VKRVIEDLSGPYIWVPARERL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109752
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109752.1, NP_001103222.1
• RefSeq Size: 3330 bp
• RefSeq ORF: 2166 bp
• Locus ID: 13385
• UniProt ID: Q62108
• Cytogenetics: 11 B3
• MW: 80.6 kDa
• Gene Summary: Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (Probable).[UniProtKB/Swiss-Prot Function]