Esrp2 (NM_176838) Mouse Tagged ORF Clone

CAT#: MR210251

Esrp2 (Myc-DDK-tagged) - Mouse epithelial splicing regulatory protein 2 (Esrp2)

Product Images

Circular map for MR210251

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Esrp2
• Synonyms: 9530027K23Rik; Rbm35b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210251 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCCGCCGCCGCCGCCGCCCCCACCCCCGGGCCCAGATCCCGCAGTAGACTCGGCCACAGACCCTT
  GTCCCGAACCTCAGTCGCTGGTGGTTTTGTTCGGGGCCACTGCCGGTGCGCTGGGGCCGGACCTAGGCTC
  GGACGAGACCGACTTAATCCTCCTAGTTTGGCAAGTGGTGGAGCCGCGCAGCCGACAGGTGGGGACGCTG
  CACAAGTCGCTAGTTCGCGCCGAGGCGGCTGCCCTGAGTCCACAATGCCGCGAGGCGAGTGGACTCAGCG
  CAGACAGCCTAGCGCGGGCGGAGTCCCTGGACAAAGTGCTGCAACAGTTCTCACAGCTGGTGAGCGGGGA
  TGTGGCTCTGCTGGGCGGGGGCCCCTATGTGCTCTGCACTGATGGGCAGCAGCTGTTGCGACAGGTTCTG
  CATCCTGAGGCCTCCAGGAAGAACCTGGTGCTCCCCGACACCTTCTTCTCCTTCTACGACCTCCGCAGAG
  AATTCCATATGCAGCACCCAAGCACCTGCTCCGCCAGAGACCTCACAGTGGGCACCATGGCACAGGACTT
  GGGACTAGAAACAGATGCTACCGAAGATGACTTTGGGGTCTGGGAAGTGAAGACTATGGTAGCTGTTATT
  CTCCACCTACTTGAAGGGTCCAATGGTCAGTTGTTCTCGAAGCCAGAAGTGGTAAAGCAGAAATACGAGA
  CAGGCCCTTGCAGCAAGGCTGATGTGGTGGACAATGAGACTGTAGTACGGGCCCGTGGGTTGCCCTGGCA
  ATCATCAGACCAGGATGTGGCTCGATTCTTCAAAGGGCTCAACATTGCCAGGGGTGGTGTGGCCCTCTGT
  CTCAACGCCCAGGGCCGCAGAAATGGCGAGGCCCTCATCCGATTCGTGGACAGCGAGCAGCGGGACCTAG
  CGCTGCAGAGACACAAACACCACATGGGTGTCCGCTATATTGAGGTATATAAAGCCACAGGGGAGGAATT
  CGTAAAGATTGCAGGGGGCACATCACTAGAGGTGGCCCGTTTCCTATCACGGGAAGATCAAGTGATCCTG
  AGGCTACGGGGACTACCCTTTTCAGCCGGGCCAACAGATGTTCTAGGCTTCCTGGGGCCAGAATGCCCAG
  TGACTGGGGGTGCTGATGGGCTGCTCTTTGTCCGCCACCCTGATGGCAGGCCTACTGGGGATGCCTTTGC
  TCTCTTTGCCTGTGAGGAGCTGGCACAGGCTGCTCTGCGCAGGCACAAAGGCATGCTGGGTAAGCGATAC
  ATTGAACTCTTCAGGAGCACGGCAGCCGAGGTGCAGCAGGTCCTTAACCGCTATGCAGCCAGTCCACTCC
  TTCCCACACTGACTGCTCCACTGCTGCCCATTCCCTTTCCACTGGCAGGAGGAACTGGGAGGGACTGTGT
  TCGCCTTAGAGGCCTGCCCTACACAGCCACCATTGAAGACATTCTGAGTTTTCTAGGAGAGGCAGCAGCT
  GACATTCGGCCTCATGGTGTGCATATGGTGCTCAACCAACAGGGCCGGCCATCCGGTGACGCCTTCATCC
  AGATGATGTCGGTGGAGCGTGCTCTGGCTGCTGCCCAGCGATGCCACAAGAAGATGATGAAAGAACGCTA
  TGTGGAAGTAGTCCCTTGTTCCACAGAGGAGATGAGCCGTGTGCTGATGGGGGGTTCCCTGAGCCGCAGT
  GGCCTGTCCCCTCCACCCTGCAAACTGCCCTGCCTCTCACCACCTACCTACGCCACCTTCCAGGCCACCC
  CAGCCCTCATTCCCACAGAGACGACAGCACTATATCCCTCTTCTGCACTGCTGCCAGCTGCCAGGGTACC
  CGCTGCTGCCACTCCTCTTGCCTATTACCCAGGGCCAGCCACTCAACTCTACATGAACTACACAGCCTAC
  TATCCCAGCCCCCCAGTCTCCCCCACTACTGTGGGCTACCTCACCACACCTCCTACTGCCTTGGCCTCTA
  CGCCCACCACAATGCTGTCCCAACCAGGAGCCCTGGTCCGAATGCAGGGGGTGCCATACACAGCTGGTAT
  GAAGGACCTACTCAGTGTCTTTCAAGCCTACCAGCTGGCTCCTGATGACTACACCACTCTGATGCCTGTT
  GGTGACCCACCTCGCACTGTGTTACAAGCCCCTAAGGAGTGGGTGTGTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210251 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTPPPPPPPPPGPDPAVDSATDPCPEPQSLVVLFGATAGALGPDLGSDETDLILLVWQVVEPRSRQVGTL
  HKSLVRAEAAALSPQCREASGLSADSLARAESLDKVLQQFSQLVSGDVALLGGGPYVLCTDGQQLLRQVL
  HPEASRKNLVLPDTFFSFYDLRREFHMQHPSTCSARDLTVGTMAQDLGLETDATEDDFGVWEVKTMVAVI
  LHLLEGSNGQLFSKPEVVKQKYETGPCSKADVVDNETVVRARGLPWQSSDQDVARFFKGLNIARGGVALC
  LNAQGRRNGEALIRFVDSEQRDLALQRHKHHMGVRYIEVYKATGEEFVKIAGGTSLEVARFLSREDQVIL
  RLRGLPFSAGPTDVLGFLGPECPVTGGADGLLFVRHPDGRPTGDAFALFACEELAQAALRRHKGMLGKRY
  IELFRSTAAEVQQVLNRYAASPLLPTLTAPLLPIPFPLAGGTGRDCVRLRGLPYTATIEDILSFLGEAAA
  DIRPHGVHMVLNQQGRPSGDAFIQMMSVERALAAAQRCHKKMMKERYVEVVPCSTEEMSRVLMGGSLSRS
  GLSPPPCKLPCLSPPTYATFQATPALIPTETTALYPSSALLPAARVPAAATPLAYYPGPATQLYMNYTAY
  YPSPPVSPTTVGYLTTPPTALASTPTTMLSQPGALVRMQGVPYTAGMKDLLSVFQAYQLAPDDYTTLMPV
  GDPPRTVLQAPKEWVCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176838
• ORF Size: 2154 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176838.2, NP_789808.1
• RefSeq Size: 2686 bp
• RefSeq ORF: 2154 bp
• Locus ID: 77411
• UniProt ID: Q8K0G8
• Cytogenetics: 8 D3
• MW: 77.4 kDa
• Gene Summary: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2 (By similarity).[UniProtKB/Swiss-Prot Function]