Slc27a4 (NM_011989) Mouse Tagged ORF Clone
CAT#: MR209717
- TrueORF®
Slc27a4 (Myc-DDK-tagged) - Mouse solute carrier family 27 (fatty acid transporter), member 4 (Slc27a4)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_011989" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Slc27a4 |
Synonyms | BB144259; FATP4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209717 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTACTTGGAGCATCTCTGGTGGGGGCGCTACTGTTCTCCAAGCTAGTGCTGAAGCTGCCCTGGACCC AGGTGGGATTCTCCCTGTTGCTCCTGTACTTGGGGTCTGGTGGCTGGCGTTTCATCCGGGTCTTCATCAA GACGGTCAGGAGAGATATCTTTGGTGGCATGGTGCTCCTGAAGGTGAAGACCAAGGTGCGACGGTACCTT CAGGAGCGGAAGACGGTGCCCCTGCTGTTTGCTTCAATGGTACAGCGCCACCCGGACAAGACAGCCCTGA TTTTCGAGGGCACAGACACTCACTGGACCTTCCGCCAGCTGGATGAGTACTCCAGTAGTGTGGCCAACTT CCTGCAGGCCCGGGGCCTGGCCTCAGGCAATGTAGTTGCCCTCTTTATGGAAAACCGCAATGAGTTTGTG GGTCTGTGGCTAGGCATGGCCAAGCTGGGCGTGGAGGCGGCTCTCATCAACACCAACCTTAGGCGGGATG CCCTGCGCCACTGTCTTGACACCTCAAAGGCACGAGCTCTCATCTTTGGCAGTGAGATGGCCTCAGCTAT CTGTGAGATCCATGCTAGCCTGGAGCCCACACTCAGCCTCTTCTGCTCTGGATCCTGGGAGCCCAGCACA GTGCCCGTCAGCACAGAGCATCTGGACCCTCTTCTGGAAGATGCCCCGAAGCACCTGCCCAGTCACCCAG ACAAGGGTTTTACAGATAAGCTCTTCTACATCTACACATCGGGCACCACGGGGCTACCCAAAGCTGCCAT TGTGGTGCACAGCAGGTATTATCGTATGGCTTCCCTGGTGTACTATGGATTCCGCATGCGGCCTGATGAC ATTGTCTATGACTGCCTCCCCCTCTACCACTCAGCAGGAAACATCGTGGGGATTGGCCAGTGCTTACTCC ACGGCATGACTGTGGTGATCCGGAAGAAGTTCTCAGCCTCCCGGTTCTGGGATGATTGTATCAAGTACAA CTGCACAATTGTACAGTACATTGGCGAGCTCTGCCGCTACCTCCTGAACCAGCCACCCCGTGAGGCTGAG TCTCGGCACAAGGTGCGCATGGCACTGGGCAACGGTCTCCGGCAGTCCATCTGGACCGACTTCTCCAGCC GTTTCCACATCCCCCAGGTGGCTGAGTTCTATGGGGCCACTGAATGCAACTGTAGCCTGGGCAACTTTGA CAGCCGGGTGGGGGCCTGTGGCTTCAATAGCCGCATCCTGTCCTTTGTGTACCCTATCCGTTTGGTACGT GTCAATGAGGATACCATGGAACTGATCCGGGGACCCGATGGAGTCTGCATTCCCTGTCAACCAGGTCAGC CAGGCCAGCTGGTGGGTCGCATCATCCAGCAGGACCCTCTGCGCCGTTTCGACGGGTACCTCAACCAGGG TGCCAACAACAAGAAGATTGCTAATGATGTCTTCAAGAAGGGGGACCAAGCCTACCTCACTGGTGACGTC CTGGTGATGGATGAGCTGGGTTACCTGTACTTCCGAGATCGCACTGGGGACACGTTCCGCTGGAAAGGGG AGAATGTATCTACCACTGAGGTGGAGGGCACACTCAGCCGCCTGCTTCATATGGCAGATGTGGCAGTTTA TGGTGTTGAGGTGCCAGGAACTGAAGGCCGAGCAGGAATGGCTGCCGTTGCAAGTCCCATCAGCAACTGT GACCTGGAGAGCTTTGCACAGACCTTGAAAAAGGAGCTGCCTCTGTATGCCCGCCCCATCTTCCTGCGCT TCTTGCCTGAGCTGCACAAGACAGGGACCTTCAAGTTCCAGAAGACAGAGTTGCGGAAGGAGGGCTTTGA CCCATCTGTTGTGAAAGACCCGCTGTTCTATCTGGATGCTCGGAAGGGCTGCTACGTTGCACTGGACCAG GAGGCCTATACCCGCATCCAGGCAGGCGAGGAGAAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209717 protein sequence
Red=Cloning site Green=Tags(s) MLLGASLVGALLFSKLVLKLPWTQVGFSLLLLYLGSGGWRFIRVFIKTVRRDIFGGMVLLKVKTKVRRYL QERKTVPLLFASMVQRHPDKTALIFEGTDTHWTFRQLDEYSSSVANFLQARGLASGNVVALFMENRNEFV GLWLGMAKLGVEAALINTNLRRDALRHCLDTSKARALIFGSEMASAICEIHASLEPTLSLFCSGSWEPST VPVSTEHLDPLLEDAPKHLPSHPDKGFTDKLFYIYTSGTTGLPKAAIVVHSRYYRMASLVYYGFRMRPDD IVYDCLPLYHSAGNIVGIGQCLLHGMTVVIRKKFSASRFWDDCIKYNCTIVQYIGELCRYLLNQPPREAE SRHKVRMALGNGLRQSIWTDFSSRFHIPQVAEFYGATECNCSLGNFDSRVGACGFNSRILSFVYPIRLVR VNEDTMELIRGPDGVCIPCQPGQPGQLVGRIIQQDPLRRFDGYLNQGANNKKIANDVFKKGDQAYLTGDV LVMDELGYLYFRDRTGDTFRWKGENVSTTEVEGTLSRLLHMADVAVYGVEVPGTEGRAGMAAVASPISNC DLESFAQTLKKELPLYARPIFLRFLPELHKTGTFKFQKTELRKEGFDPSVVKDPLFYLDARKGCYVALDQ EAYTRIQAGEEKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011989 |
ORF Size | 1932 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011989.5 |
RefSeq Size | 4008 bp |
RefSeq ORF | 1932 bp |
Locus ID | 26569 |
UniProt ID | Q91VE0 |
Cytogenetics | 2 20.64 cM |
MW | 72.3 kDa |
Gene Summary | Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. Appears to be the principal fatty acid transporter in small intestinal enterocytes. Plays a role in the formation of the epidermal barrier. Required for fat absorption in early embryogenesis. Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids (VLCFAs). Indirectly inhibits RPE65 via substrate competition and via production of VLCFA derivatives like lignoceroyl-CoA. Prevents light-induced degeneration of rods and cones.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG209717 | Slc27a4 (tGFP-tagged) - Mouse solute carrier family 27 (fatty acid transporter), member 4 (Slc27a4) |
USD 799.00 |
|
MR209717L3 | Lenti ORF clone of Slc27a4 (Myc-DDK-tagged) - Mouse solute carrier family 27 (fatty acid transporter), member 4 (Slc27a4) |
USD 899.00 |
|
MR209717L4 | Lenti ORF clone of Slc27a4 (mGFP-tagged) - Mouse solute carrier family 27 (fatty acid transporter), member 4 (Slc27a4) |
USD 899.00 |
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