Epn2 (NM_010148) Mouse Tagged ORF Clone
CAT#: MR209265
- TrueORF®
Epn2 (Myc-DDK-tagged) - Mouse epsin 2 (Epn2)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_010148" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Epn2 |
Synonyms | 9530051D10Rik; AA536924; Ibp2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209265 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACTTCATCTATCAGACGGCAGATGAAAAACATTGTGAACAATTACTCAGAGGCTGAAATCAAAG TCCGGGAGGCCACCTCCAATGACCCGTGGGGCCCATCCAGCTCTCTGATGACTGAGATTGCTGACCTGAC CTACAATGTGGTAGCGTTCTCGGAGATCATGAGCATGGTTTGGAAGCGACTTAATGACCATGGCAAGAAC TGGCGGCATGTATACAAGGCACTGACACTGCTGGACTACCTTATCAAGACAGGTTCTGAGCGGGTGGCCC AGCAGTGCCGTGAGAACATCTTTGCCATACAGACTCTGAAGGACTTTCAGTACATTGACCGTGATGGCAA AGACCAGGGTATTAACGTTCGAGAGAAATCAAAGCAACTGGTTGCGCTCCTCAAGGATGAGGAGCGGCTG AAGGTTGAGAGAGTTCAGGCTCTCAAAACCAAAGAGCGCATGGCTCAGGTGGCCACTGGCGTGGGCAGCA ACCAAATCACCTTTGGCCGTGGCTCCAGCCAGCCCAACCTTTCTACCAGCTACTCAGAGCAGGAGTATGG CAAGGCTGGGGGCTCGCCGGCATCCTACCACGGCTCTACTTCCCCACGAGTATCCTCTGAGTTAGAGCAG GCCCGGCCACAGACAAGCGGAGAAGAGGAGCTGCAGCTACAACTGGCACTTGCCATGAGCAGAGAGGTTG CTGAGCAGTCATCAGAAAGCGTGCAGACAGCCAGAGGCAGCAAGGAAGAACGCCTCAGGCGGGGTGATGA CCTCAGATTGCAGATGGCTCTGGAAGAAAGCCGGAGAGACACAGTAAAAGTTCCAAAAAAGAAAGAGGCG AAAGCTTGTTGCAAGCCAGGCTCCCACTCCCAGCAGACTACCTTGTTGGATTTAATGGATGCCCTCCCCA GCTCAGGCCCTGTTACACAGAAAACTGAGCCCTGGAGTGCAGGAGCCTCTGCTAACCAGACCAACCCCTG GGGTGGAACAGTGGCTCCTTCGAACATTACTGACCCGTGGCCATCGTTTGGTACCAAGCCAGCTGCCTCT GTGGACCCCTGGGGAGTACCTACCACAGCCAGCACACAGTCTGTCCCTAAGAACTCAGACCCTTGGGCAG CCTCACAGCAGCCTGCCTCCAATGCTGGAAAGACAACAGATGCCTGGGGAGCTGCTAAGCCCAGTTCTGC CTCAGGGTCCTTTGAGCTCTTCAGTAATTTCAACGGTACAGTTAAAGATGATTTTTCTGAATTTGACAAC CTTCGAACTTCAAAAAAACCAGCTGAGTCAGGGGCCTCAGTGCCACCCCAGGACAGCAGAACCACGAGCC CTGACCTCTTTGAGTCTCAATCCTTGACTTCTGCGTCAAGCAAGCCTAGCAGTGCCAGGAAAACACCTGA GTCCTTCCTGGGCCCCAATGCAGCCCTGGTGAACCTGGACTCATTGGTGACTAAGCCTGCTCCACCAGCT CAGTCCCTTAATCCCTTCCTGGCACCAGGTGCTGCTGCTCCAGCCCCTGTCAACCCCTTCCAGGTCAACC AGCCCCAGCCGCTGACACTGAACCAGCTCCGGGGAAGCCCTGTCCTGGGAAGCAGTGCATCCTTTGGGTC TGGTCCAGGGGTGGAGACTGTGGCTCCCATGACCTCAGTAGCTCCACACTCATCAGTGGGGGCCAGTGGC TCCTCCTTGACACCACTGGGCCCTACAGCAATGAACATGGTAGGCAGTGTGGGCATTCCCCCATCAGCAG CTCAGTCAACAGGCACAACAAACCCTTTCCTTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209265 protein sequence
Red=Cloning site Green=Tags(s) MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMVWKRLNDHGKN WRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERL KVERVQALKTKERMAQVATGVGSNQITFGRGSSQPNLSTSYSEQEYGKAGGSPASYHGSTSPRVSSELEQ ARPQTSGEEELQLQLALAMSREVAEQSSESVQTARGSKEERLRRGDDLRLQMALEESRRDTVKVPKKKEA KACCKPGSHSQQTTLLDLMDALPSSGPVTQKTEPWSAGASANQTNPWGGTVAPSNITDPWPSFGTKPAAS VDPWGVPTTASTQSVPKNSDPWAASQQPASNAGKTTDAWGAAKPSSASGSFELFSNFNGTVKDDFSEFDN LRTSKKPAESGASVPPQDSRTTSPDLFESQSLTSASSKPSSARKTPESFLGPNAALVNLDSLVTKPAPPA QSLNPFLAPGAAAPAPVNPFQVNQPQPLTLNQLRGSPVLGSSASFGSGPGVETVAPMTSVAPHSSVGASG SSLTPLGPTAMNMVGSVGIPPSAAQSTGTTNPFLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010148 |
ORF Size | 1788 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010148.4 |
RefSeq Size | 4266 bp |
RefSeq ORF | 1788 bp |
Locus ID | 13855 |
UniProt ID | Q8CHU3 |
Cytogenetics | 11 B2 |
MW | 63.5 kDa |
Gene Summary | Plays a role in the formation of clathrin-coated invaginations and endocytosis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204572 | Epn2 (untagged) - Mouse epsin 2 (Epn2), (10ug) |
USD 555.00 |
|
MR209265L3 | Lenti ORF clone of Epn2 (Myc-DDK-tagged) - Mouse epsin 2 (Epn2) |
USD 855.00 |
|
MR209265L4 | Lenti ORF clone of Epn2 (mGFP-tagged) - Mouse epsin 2 (Epn2) |
USD 855.00 |
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