Zmynd11 (BC058999) Mouse Tagged ORF Clone

CAT#: MR208886

Zmynd11 (Myc-DDK-tagged) - Mouse zinc finger, MYND domain containing 11 (cDNA clone MGC:69582 IMAGE:6827880)

Product Images

Circular map for MR208886

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zmynd11
• Synonyms: 2210402G22Rik; BS69
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208886 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGAGTCCACGGTATGCATCCCAAAGAGACCACCCGCCAGCTGAGCTTAGCTGTGAAAGATGGTC
  TTATTGTCGAAACTCTAACAGTGGGCTGCAAAGGTTCAAAAGCTGGTATTGAACAGGAAGGATATTGGTT
  ACCAGGAGATGAGATTGACTGGGAAACAGAAACTCATGACTGGTATTGTTTTGAATGCCATTTGCCTGGA
  GAGGTGTTGATATGTGACCTGTGTTTTCGTGTGTATCATTCCAAGTGTTTGTCTGATGAGTTCAGGCTTA
  GAGACAGCAGTAGTCACTGGCAGTGCCCAGTTTGCAGGAGCATTAAGAAGAAGCACTCTAACAAGCAGGA
  AATGGGCACCTACTTGAGGTTCATCGTCTCCCGAATGAAGGAGCGGGCTATAGACCTTAATAAGAAAGGA
  AAGGACAGTAAGCATCCTATGTACCGGAGGCTGGTCCACTCAGCTGTTGACGTCCCTACCATACAAGAGA
  AGGTGAATGAGGGGAAGTACCGGAGTTACGAGGAGTTCAAGGCTGACGCACAGCTGCTTCTCCACAACAC
  AGTGATCTTCTATGGAGACAGTGAGCAGGCGGACATTGCACGGATGTTATATAAAGACACGTGTCACGAG
  CTGGACGAGCTGCAGCTTTGCAAGAACTGCTTCTATCTGTCAAACGCACGGCCCGACAACTGGTTCTGCT
  ACCCTTGCATACCTAATCATGAGCTGGTTTGGGCTAAAATGAAAGGTTTTGGGTTTTGGCCAGCCAAAGT
  CATGCAGAAAGAAGACAATCAAGTGGATGTTCGCTTCTTTGGCCATCACCACCAGAGAGCCTGGATTCCT
  TCTGAAAACATCCAGGATATCACGGTCAATGTTCACCGACTGCACGTGAAGCGCAGCATGGGCTGGAAGA
  AGGCCTGTGACGAGCTGGAACTGCATCAGCGCTTCCTCCGTGAAGGAAGGTTCTGGAAGTCTAAGAATGA
  GGACCGAGGTGAGGAAGAGGCAGAATCCAGTATCTCCTCCACCAGTAACGAGCAGCTGAAGGTCACTCAA
  GAGCCAAGAGCAAAGAAAGGACGACGTAATCAGAGTGTGGAGCCCAAAAAGGAAGAACCCGAGCCAGAAA
  CGGAAGCAGTGAGTTCCAGCCAGGAAATCCCCACAATGCCTCAGCCAATTGAGAGGGTGTCAGTGTCAAC
  CCAGACCAAGAAGTTAAGTGCCTCTTCCCCACGAATGCTGCATCGAAGCACTCAGACGACGAGCGACGGC
  GTCTGTCAGAGCATGTGCCACGACAAGTACACCAAAATTTTTAATGACTTTAAAGACAGGATGAAGTCGG
  ACCACAAGCGGGAAACAGAGAGAGTGGTCCGTGAAGCGCTGGAAAAGTTGCGTTCTGAAATGGAAGAAGA
  AAAAAGACAGGCCGTGAACAAAGCTGTGGCCAGCCTGCAGGGCGACATGGACAGAAAGGGTAAGCAGCTG
  AAGGAGAAGTGCAAGGAAGAGTTTGTAGAGGAGATCAAGAAGCTAGCGGCGCAGCACAAGCAGCTCATTT
  CCCAGACCAAGAAGAAGCAGTGGTGCTACAACTGTGAGGAGGAGGCCATGTACCACTGCTGCTGGAACAC
  GTCCTACTGCTCCATCAAGTGCCAGCAGGAGCACTGGCACGCAGAGCACAAGCGCACCTGCCGCCGGAAG
  AGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208886 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRVHGMHPKETTRQLSLAVKDGLIVETLTVGCKGSKAGIEQEGYWLPGDEIDWETETHDWYCFECHLPG
  EVLICDLCFRVYHSKCLSDEFRLRDSSSHWQCPVCRSIKKKHSNKQEMGTYLRFIVSRMKERAIDLNKKG
  KDSKHPMYRRLVHSAVDVPTIQEKVNEGKYRSYEEFKADAQLLLHNTVIFYGDSEQADIARMLYKDTCHE
  LDELQLCKNCFYLSNARPDNWFCYPCIPNHELVWAKMKGFGFWPAKVMQKEDNQVDVRFFGHHHQRAWIP
  SENIQDITVNVHRLHVKRSMGWKKACDELELHQRFLREGRFWKSKNEDRGEEEAESSISSTSNEQLKVTQ
  EPRAKKGRRNQSVEPKKEEPEPETEAVSSSQEIPTMPQPIERVSVSTQTKKLSASSPRMLHRSTQTTSDG
  VCQSMCHDKYTKIFNDFKDRMKSDHKRETERVVREALEKLRSEMEEEKRQAVNKAVASLQGDMDRKGKQL
  KEKCKEEFVEEIKKLAAQHKQLISQTKKKQWCYNCEEEAMYHCCWNTSYCSIKCQQEHWHAEHKRTCRRK
  R
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC058999
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058999, AAH58999
• RefSeq Size: 4107 bp
• RefSeq ORF: 1685 bp
• Locus ID: 66505
• Cytogenetics: 13 A1
• MW: 66 kDa
• Gene Summary: Chromatin reader that specifically recognizes and binds histone H3.3 trimethylated at 'Lys-36' (H3.3K36me3) and regulates RNA polymerase II elongation. Does not bind other histone H3 subtypes (H3.1 or H3.2). Colocalizes with highly expressed genes and functions as a transcription corepressor by modulating RNA polymerase II at the elongation stage (PubMed:24590075). Binds non-specifically to dsDNA (By similarity). Acts as a tumor-suppressor by repressing a transcriptional program essential for tumor cell growth (PubMed:24590075).[UniProtKB/Swiss-Prot Function]