Tbc1d17 (BC017607) Mouse Tagged ORF Clone

CAT#: MR208694

Tbc1d17 (Myc-DDK-tagged) - Mouse TBC1 domain family, member 17 (cDNA clone MGC:27642 IMAGE:4507589)

Product Images

Circular map for MR208694

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tbc1d17
• Synonyms: MGC27642
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208694 representing BC017607
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGATCCAGCTACAGGGTGGTGTTTGAAAAGGGAGGCGTGTACCTGCACACCAGCGCCCGGAAAC
  ACCAGGATCCAGACTCGCTCATCGCCGGCGTCATACGAGTGGTGGAAAAGGACAGCGACGTCTTTCTGCA
  CTGGGCTCCGGTGGAGGAGGCTGGAGACCCAACCCAGATTCTCTTCAAGAAGGACCCGAGTCGGGGTGAG
  CCCAGCACCTCAGAGGAGGAACCTACCTTTGACCCAGGCTATGAACCTGACTGGGCTGTCATTAGCACGG
  TGCGGCCACAGCCCCACCTGGCAGAGCCCAGGAAAGGTGCAGAGCCCAGTTCCTCCCGGAGCTCCTGGGC
  CTTCTCCGTGAGCCTGGGGGAGCTCAAGTCCATCCGCAGATCCAAGCCAGGCCTCAGCTGGGCCTACCTG
  GTCCTGGTGACCCAGGCTGGAGGCTCCCTGCCTGCCTTACACTTTCACAGAGGGGGTACCCGAGCCCTGC
  TCCGAGTCCTTAGCCGATACCTGCTGTTGGCCAGCTCTCCACAGGACTCCCGCCTCTACTTGGTCTTCCC
  CCAGGACCCCTCTGCCCTGTCCGACTCCTTCCACCACCTGCAGCTCTTTGACCAGGACAGCTCCAATGTG
  GTGTCTCGTTTCCTTCAGGACCCCTACTCCACCACCTTCAGTAGCTTCTCTCGAGTGACCAACTTCTTCC
  GGGGAGCCCTACAGCCGCACCCTGAGGGCGCCTCCTCCCCTAACCTACCCCCGCTGCCAGATGATGAACC
  AGAGCCTGGCTTTGAGGTCATTTCTTGCGTGGAGCTGGGGCAGCGTCCCACAGTGGAGCGTGCTCCTCCA
  GTCACAGAGGAAGAGTGGAACCGCTACGTGGGCCCCGAGGGTCGCCTGCAGAATGTCCCTGAGTTGAAAA
  ACCGGATCTTCTCAGGCGGTCTCAGCCCTGGCCTACGTCGAGAGGCTTGGAAGTTCCTTCTGGGGTACCT
  TAGCTGGGAGAGCTCAGCTGAAGAGCACAAGGCGCATGTGCGGAAGAAAACGGATGAGTACTTCCGAATG
  AAGCTGCAGTGGAAGTCTGTGAGTGCTGAGCAGGAGCGGAGGAACTCACTGCTGCATGGATACCGAAGCC
  TCATTGAGAGAGATGTAAGCCGCACGGACAGGACTAACAAGTTCTATGAAGGCCCTGAGAACCCTGGGCT
  GAGCCTGCTCCATGATATCCTTCTTACCTACTGCATGTACCACTTCGACTTGGGCTACGTTCAGGGTATG
  AGTGACCTCCTGTCCCCGATCCTCTTCGTTGTTCAGAACGAAGTAGATGCCTTCTGGTGTTTCTGCGGCT
  TCATGGAACTTGTGCATGGGAACTTTGAAGAGAGTCAGGAGACTATGAAGCGGCAGCTTGGGCAGCTCCT
  GCTGCTTCTGCGGGTGCTGGATCAGCCACTCTGTGATTTCCTGGATTCTCAGGACTCGGGCTCGCTGTGC
  TTCTGTTTCCGGTGGCTGCTCATCTGGTTCAAGAGGGAATTCCCCTTTCCTGATGTCCTCCGGCTGTGGG
  AGGTGGGCGGCCAGTTGGGGTGCTGTGGACAGGGCTTCCTGGCCCCAATTTGCACCTGCTGGTGGCCTGT
  GCCATCTTGGATATGGAACGGGACACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208694 representing BC017607
  Red=Cloning site Green=Tags(s)
  
  MEGSSYRVVFEKGGVYLHTSARKHQDPDSLIAGVIRVVEKDSDVFLHWAPVEEAGDPTQILFKKDPSRGE
  PSTSEEEPTFDPGYEPDWAVISTVRPQPHLAEPRKGAEPSSSRSSWAFSVSLGELKSIRRSKPGLSWAYL
  VLVTQAGGSLPALHFHRGGTRALLRVLSRYLLLASSPQDSRLYLVFPQDPSALSDSFHHLQLFDQDSSNV
  VSRFLQDPYSTTFSSFSRVTNFFRGALQPHPEGASSPNLPPLPDDEPEPGFEVISCVELGQRPTVERAPP
  VTEEEWNRYVGPEGRLQNVPELKNRIFSGGLSPGLRREAWKFLLGYLSWESSAEEHKAHVRKKTDEYFRM
  KLQWKSVSAEQERRNSLLHGYRSLIERDVSRTDRTNKFYEGPENPGLSLLHDILLTYCMYHFDLGYVQGM
  SDLLSPILFVVQNEVDAFWCFCGFMELVHGNFEESQETMKRQLGQLLLLLRVLDQPLCDFLDSQDSGSLC
  FCFRWLLIWFKREFPFPDVLRLWEVGGQLGCCGQGFLAPICTCWWPVPSWIWNGTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC017607
• ORF Size: 1638 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC017607.1
• RefSeq Size: 2376 bp
• RefSeq ORF: 1640 bp
• Locus ID: 233204
• Cytogenetics: 7 B3
• MW: 87.1 kDa
• Gene Summary: Probable GTPase-activating protein for Rab8; its transient association with Rab8 is mediated by OPTN. Inhibits Rab8-mediated endocytic trafficking, such as of transferrin receptor (TfR) and reduces Rab8 recruitnment to tubules emanating from the endocytic recycling compartment (ERC). Involved in regulation of autophagy. Mediates inhibition of autophagy caused by the OPTN variant GLC1E LYS-50; the function requires its catalytic activity, however, the involved Rab is not known (By similarity).[UniProtKB/Swiss-Prot Function]