Rtn4r (NM_022982) Mouse Tagged ORF Clone

CAT#: MR207571

Rtn4r (Myc-DDK-tagged) - Mouse reticulon 4 receptor (Rtn4r)

Product Images

Circular map for MR207571

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rtn4r
• Synonyms: NgR; NgR1; NOGOR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207571 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGGGCGTCCTCCGGAGGAAGCAGGCTGCTGGCATGGGTGTTATGGCTACAGGCCTGGAGGGTAG
  CAACACCATGCCCTGGTGCTTGTGTGTGCTACAATGAGCCCAAGGTAACAACAAGCTGCCCCCAGCAGGG
  TCTGCAGGCTGTGCCCACTGGCATCCCAGCCTCTAGCCAGCGAATCTTCCTGCATGGCAACCGAATCTCT
  CACGTGCCAGCTGCGAGCTTCCAGTCATGCCGAAATCTCACTATCCTGTGGCTGCACTCTAATGCGCTGG
  CTCGGATCGATGCTGCTGCCTTCACTGGTCTGACCCTCCTGGAGCAACTAGATCTTAGTGATAATGCACA
  GCTTCATGTCGTGGACCCTACCACGTTCCACGGCCTGGGCCACCTGCACACACTGCACCTAGACCGATGT
  GGCCTGCGGGAGCTGGGTCCCGGCCTATTCCGTGGACTAGCAGCTCTGCAGTACCTCTACCTACAAGACA
  ACAATCTGCAGGCACTCCCTGACAACACCTTTCGAGACCTGGGCAACCTCACGCATCTCTTTCTGCATGG
  CAACCGTATCCCCAGTGTGCCTGAGCACGCTTTCCGTGGCCTGCACAGTCTTGACCGCCTCCTCTTGCAC
  CAGAACCATGTGGCTCGTGTGCACCCACATGCCTTCCGGGACCTTGGCCGCCTCATGACCCTCTACCTGT
  TTGCCAACAACCTCTCCATGCTGCCTGCAGAGGTCCTAATGCCCCTGAGGTCTCTGCAGTACCTGCGACT
  CAATGACAACCCCTGGGTGTGTGACTGCCGGGCACGTCCACTCTGGGCCTGGCTGCAGAAGTTCCGAGGT
  TCCTCATCAGAGGTGCCCTGCAACCTGCCCCAACGCCTGGCAGACCGTGATCTTAAGCGCCTCGCTGCCA
  GTGACCTAGAGGGCTGTGCTGTGGCTTCAGGACCCTTCCGTCCCATCCAGACCAGTCAGCTCACTGATGA
  GGAGCTGCTGAGCCTCCCCAAGTGCTGCCAGCCAGATGCTGCAGACAAAGCCTCAGTACTGGAACCCGGG
  AGGCCAGCTTCTGCCGGAAACGCCCTCAAGGGACGTGTGCCTCCCGGTGACACTCCACCAGGCAATGGCT
  CAGGCCCTCGGCACATCAATGACTCTCCATTTGGAACTTTGCCCAGCTCTGCAGAGCCCCCACTGACTGC
  CCTGCGGCCTGGGGGTTCCGAGCCACCAGGACTTCCCACCACTGGTCCCCGCAGGAGGCCAGGTTGTTCC
  CGGAAGAATCGCACCCGCAGCCACTGCCGTCTGGGCCAGGCGGGAAGTGGGGCCAGTGGAACAGGGGACG
  CAGAGGGTTCAGGGGCTCTGCCTGCTCTGGCCTGCAGCCTTGCTCCTCTGGGCCTTGCACTGGTACTTTG
  GACAGTGCTTGGGCCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207571 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKRASSGGSRLLAWVLWLQAWRVATPCPGACVCYNEPKVTTSCPQQGLQAVPTGIPASSQRIFLHGNRIS
  HVPAASFQSCRNLTILWLHSNALARIDAAAFTGLTLLEQLDLSDNAQLHVVDPTTFHGLGHLHTLHLDRC
  GLRELGPGLFRGLAALQYLYLQDNNLQALPDNTFRDLGNLTHLFLHGNRIPSVPEHAFRGLHSLDRLLLH
  QNHVARVHPHAFRDLGRLMTLYLFANNLSMLPAEVLMPLRSLQYLRLNDNPWVCDCRARPLWAWLQKFRG
  SSSEVPCNLPQRLADRDLKRLAASDLEGCAVASGPFRPIQTSQLTDEELLSLPKCCQPDAADKASVLEPG
  RPASAGNALKGRVPPGDTPPGNGSGPRHINDSPFGTLPSSAEPPLTALRPGGSEPPGLPTTGPRRRPGCS
  RKNRTRSHCRLGQAGSGASGTGDAEGSGALPALACSLAPLGLALVLWTVLGPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022982
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022982.3
• RefSeq Size: 1890 bp
• RefSeq ORF: 1422 bp
• Locus ID: 65079
• UniProt ID: Q99PI8
• Cytogenetics: 16 A3
• MW: 51 kDa
• Gene Summary: Receptor for RTN4, OMG and MAG (PubMed:11201742, PubMed:12089450, PubMed:15504325, PubMed:18411262, PubMed:22923615). Functions as receptor for the sialylated gangliosides GT1b and GM1 (PubMed:18411262). Besides, functions as receptor for chondroitin sulfate proteoglycans (PubMed:22406547). Can also bind heparin (PubMed:22406547). Intracellular signaling cascades are triggered via the coreceptor NGFR (By similarity). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed:22325200). Mediates axonal growth inhibition (By similarity). Mediates axonal growth inhibition and plays a role in regulating axon regeneration and neuronal plasticity in the adult central nervous system (PubMed:11201742, PubMed:12089450, PubMed:15504325, PubMed:22923615). Plays a role in postnatal brain development (PubMed:27339102). Required for normal axon migration across the brain midline and normal formation of the corpus callosum (PubMed:27339102). Protects motoneurons against apoptosis; protection against apoptosis is probably mediated via interaction with MAG (PubMed:26335717). Acts in conjunction with RTN4 and LINGO1 in regulating neuronal precursor cell motility during cortical development (PubMed:20093372). Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed:22325200).[UniProtKB/Swiss-Prot Function]