Chst3 (NM_016803) Mouse Tagged ORF Clone
CAT#: MR207541
- TrueORF®
Chst3 (Myc-DDK-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 3 (Chst3)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_016803" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Chst3 |
Synonyms | C6ST; C6ST-1; GST-0 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207541 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAAGGACTCGCTTTGCCTCAGGATTTCCGGGACCTTGTACACAGCCTAAAGATTCGAGGCAGAT ACGTCTTGTTCCTGGCATTTGTGGTCATAGTTTTTATCTTCATTGAAAAGGAAAATAAAATCATATCCAG GGTCTCCGACAAGCTGAAGCAGATCCCTCATTTTGTGGCAGATGCCAACAGCACTGACCCAGCCCTGCTC TTATCGGAGAATGCATCTCTCTTGTCCCTGAGCGAGTTGGATTCCACCTTTTCCCATCTGCGGAGCCGCC TGCACAACCTGAGCCTGCAGCTGGGCGTGGAGCCAGCAATGGAGAGCCAGGAGGCTGGGGCAGAGAAGCC ATCCCAGCAGGCTGGAGCAGGGACCCGGCGCCACGTGCTTCTCATGGCCACCACCCGCACGGGTTCCTCG TTCGTGGGCGAGTTCTTCAACCAGCAGGGCAATATCTTCTACCTCTTCGAGCCACTGTGGCACATCGAGC GCACCGTGTTCTTCCAGCAGCGAGGCGCCAGCGCGGCTGGTTCAGCCTTGGTCTACCGTGATGTCCTCAA GCAGTTGTTGCTATGCGACCTGTATGTGCTGGAGCCCTTCATCAGCCCTCCGCCCGAGGACCACTTGACT CAGTTCCTGTTCCGCCGGGGATCCAGCCGTTCACTCTGCGAGGATCCGGTGTGCACACCCTTCGTCAAGA AGGTCTTTGAGAAGTACCACTGCAGGAACCGTCGCTGCGGGCCACTCAACGTGACCTTGGCGGGCGAGGC CTGCCGCCGCAAGGACCACGTGGCCCTCAAGGCTGTGCGCATCCGTCAGCTGGAGTTCCTGCAGCCGCTA GTTGAGGACCCGAGGTTGGATCTACGAGTCATTCAGCTGGTGCGCGACCCCCGGGCCGTGCTGGCTTCAC GCATAGTGGCCTTTGCGGGCAAGTATGAGAACTGGAAGAAGTGGCTGTCCGAGGGGCAGGACCAGCTGAG CGAGGATGAGGTGCAGCGATTGCGGGGCAACTGTGAGAGCATCCGCCTGTCTGCAGAGCTGGGCTTGCGG CAGCCAGCCTGGCTGCGCGGTCGTTACATGCTGGTGCGCTATGAGGATGTGGCACGCAGGCCACTGCAGA AGGCCCGAGAGATGTACAGCTTTGCGGGCATCCCCTTGACCCCGCAGGTGGAGGACTGGATCCAGAAGAA CACGCAGGCGACACGCGACAGCAGCGATGTCTACTCCACTCAGAAAAACTCTTCTGAGCAGTTTGAGAAG TGGCGCTTCAGCATGCCTTTCAAGCTGGCACAGGTGGTACAGGCTGCCTGTGGCCCGACCATGCACCTCT TTGGCTACAAGTTGGCCAGGGATGCCGCCTCACTCACCAACCGCTCCATCAGCCTGCTGGAGGAGCGGGG CACCTTCTGGGTCACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207541 protein sequence
Red=Cloning site Green=Tags(s) MEKGLALPQDFRDLVHSLKIRGRYVLFLAFVVIVFIFIEKENKIISRVSDKLKQIPHFVADANSTDPALL LSENASLLSLSELDSTFSHLRSRLHNLSLQLGVEPAMESQEAGAEKPSQQAGAGTRRHVLLMATTRTGSS FVGEFFNQQGNIFYLFEPLWHIERTVFFQQRGASAAGSALVYRDVLKQLLLCDLYVLEPFISPPPEDHLT QFLFRRGSSRSLCEDPVCTPFVKKVFEKYHCRNRRCGPLNVTLAGEACRRKDHVALKAVRIRQLEFLQPL VEDPRLDLRVIQLVRDPRAVLASRIVAFAGKYENWKKWLSEGQDQLSEDEVQRLRGNCESIRLSAELGLR QPAWLRGRYMLVRYEDVARRPLQKAREMYSFAGIPLTPQVEDWIQKNTQATRDSSDVYSTQKNSSEQFEK WRFSMPFKLAQVVQAACGPTMHLFGYKLARDAASLTNRSISLLEERGTFWVT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016803 |
ORF Size | 1419 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016803.2, NP_058083.1 |
RefSeq Size | 6129 bp |
RefSeq ORF | 1437 bp |
Locus ID | 53374 |
UniProt ID | O88199 |
Cytogenetics | 10 B4 |
MW | 54 kDa |
Gene Summary | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205469 | Chst3 (untagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 3 (Chst3), (10ug) |
USD 457.00 |
|
MG207541 | Chst3 (tGFP-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 3 (Chst3) |
USD 657.00 |
|
MR207541L3 | Lenti ORF clone of Chst3 (Myc-DDK-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 3 (Chst3) |
USD 757.00 |
|
MR207541L4 | Lenti ORF clone of Chst3 (mGFP-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 3 (Chst3) |
USD 757.00 |
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