Chst3 (NM_016803) Mouse Untagged Clone

CAT#: MC205469

Chst3 (untagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 3 (Chst3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chst3
• Synonyms: C6ST; C6ST-1; GST-0
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055055
  CTTAGCCACTGAACCATCTCTCCAGCCTCTGTTTTGGGGTGTTTGTTTGAGACAGAATCAAAAGGCTGGT CTAAAGTACATATTGTAGCTAAAGATGGCTTCCAACTCCTGCCTTGCCTCCAGAGTGTTAGGGTGTGACC TTTAAATGACCTATTAAAGAAACCCCAACAGGTGTAGAGTACACCTGTGATCCCAGCTCTTAGGAGGTTG GACAGATGGATAAAGAGGTCCAGGTGTCCCGGACTACATAGAGAGACCCTATCTCAAAAGAGAGGCGAAG ACTTAGGTTTTGAGAGTGGGCCTGAGGTCTCTCTTCAAAAGTTTTATAGAAAGATGTCTCTGTTCCCTGT CTATGAACACGGATGGCCTGAGCACCTGTCTCTTCACAGGATCAGAGTGTCCCCCACCTGAAGAGGGCTG ATTGGGTCCCAAGCTATGCTCCTGAGCTGAGTGCCTGCAGCCAGTCTGAGGAACTCCATGGCGCCCCCTC TCCCCATGGAGAAAGGACTCGCTTTGCCTCAGGATTTCCGGGACCTTGTACACAGCCTAAAGATTCGAGG CAGATACGTCTTGTTCCTGGCATTTGTGGTCATAGTTTTTATCTTCATTGAAAAGGAAAATAAAATCATA TCCAGGGTCTCCGACAAGCTGAAGCAGATCCCTCATTTTGTGGCAGATGCCAACAGCACTGACCCAGCCC TGCTCTTATCGGAGAATGCATCTCTCTTGTCCCTGAGCGAGTTGGATTCCACCTTTTCCCATCTGCGGAG CCGCCTGCACAACCTGAGCCTGCAGCTGGGCGTGGAGCCAGCAATGGAGAGCCAGGAGGCTGGGGCAGAG AAGCCATCCCAGCAGGCTGGAGCAGGGACCCGGCGCCACGTGCTTCTCATGGCCACCACCCGCACGGGTT CCTCGTTCGTGGGCGAGTTCTTCAACCAGCAGGGCAATATCTTCTACCTCTTCGAGCCACTGTGGCACAT CGAGCGCACCGTGTTCTTCCAGCAGCGAGGCGCCAGCGCGGCTGGTTCAGCCTTGGTCTACCGTGATGTC CTCAAGCAGTTGTTGCTATGCGACCTGTATGTGCTGGAGCCCTTCATCAGCCCTCCGCCCGAGGACCACT TGACTCAGTTCCTGTTCCGCCGGGGATCCAGCCGTTCACTCTGCGAGGATCCGGTGTGCACACCCTTCGT CAAGAAGGTCTTTGAGAAGTACCACTGCAGGAACCGTCGCTGCGGGCCACTCAACGTGACCTTGGCGGGC GAGGCCTGCCGCCGCAAGGACCACGTGGCCCTCAAGGCTGTGCGCATCCGTCAGCTGGAGTTCCTGCAGC CGCTAGTTGAGGACCCGAGGTTGGATCTACGAGTCATTCAGCTGGTGCGCGACCCCCGGGCCGTGCTGGC TTCACGCATAGTGGCCTTTGCGGGCAAGTATGAGAACTGGAAGAAGTGGCTGTCCGAGGGGCAGGACCAG CTGAGCGAGGATGAGGTGCAGCGATTGCGGGGCAACTGTGAGAGCATCCGCCTGTCTGCAGAGCTGGGCT TGCGGCAGCCAGCCTGGCTGCGCGGTCGTTACATGCTGGTGCGCTATGAGGATGTGGCACGCAGGCCACT GCAGAAGGCCCGAGAGATGTACAGCTTTGCGGGCATCCCCTTGACCCCGCAGGTGGAGGACTGGATCCAG AAGAACACGCAGGCGACACGCGACAGCAGCGATGTCTACTCCACTCAGAAAAACTCTTCTGAGCAGTTTG AGAAGTGGCGCTTCAGCATGCCTTTCAAGCTGGCACAGGTGGTACAGGCTGCCTGTGGCCCGACCATGCA CCTCTTTGGCTACAAGTTGGCCAGGGATGCCGCCTCACTCACCAACCGCTCCATCAGCCTGCTGGAGGAG CGGGGCACCTTCTGGGTCACGTAGTGGGGGATGTCTGGGACCCTTGGAACTCCTTCTTGTGAAAGGCTGG CCCTGCTTCCCTCACACCCAGCCTGGCAGTGAGACATAGCCCTGGCAGAAAGTCAAAATGGGGAGCGATG ATGGGAACATAGCCCCTGGCTGTAGCGGTAGGGCCCCCTGCCAGCTAGACTCCCCAGAGCAGCTACAGCT AGGGCCTTGGGCTCCTCTGAGGACACCCTGTCTCCTTAGTGGTACTGGTCATAAGGTGTCCTCACCCTAT GACCTGCAGTGTCCCGAGCAGGTCAAGGTAGGTTCCTGTGTGTGGACACACCTCCCTAGCTCTTTCTCAC ACAGTCTACACGAAGCCTGTAAAGGCCTGTAGGTTGTGTGGGCTGGAGTCCAAGTATTTAACAACCAGAA GGGGTGTAGACCCTCTGCAGCCTCTCAGACCTCCTACTGTATTAAGTGTTAACCTCTTCCGCTCTGAGTC AGAGAAGCTGGGATCTGGCTGAGTCCTGGGAAGGAGGAGGACAGCCTAGGGTGAGGAAGGGGACCTTTGA AGCTCCTCAGGGAACAGTGGCTGTGTACCAGCTCATAGAAAATGGTTTGATCAGCTGTTCTAGTCACTCA TGAGTATCAATCAGCCTGTGTAGAGCAAGACACACAAAGTGCATTGAAAACAGACAAGGCCAGGGGTGTG GCTCAGCGGTAAAGCCCTTGCCTCTCTCATGCCCAGGGCCCTAGGTGTGATTCTTAGCACTATAAATATA AGGGAAAATAAGCCATACATACGTGTGCAGTACAGAGAGAGACAGAAAAGCGTAATAAACATTCCCCCCT CTCATAAGCACACGCTCTGCCTCTGTCTCTGTCTCTGTCTCTCTCTGTCTCTGTCTCTCTCTGTCTCTCT CTCTCACTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACACACACACAAACACACACACA CACACTGGTACAGAAAAGCTTCCCTTCTCCACTCTTTATGCTCACCATGTTTTTAGGATTTTTGTTTGTT TGTTTTGTTTTGGGTTTCTATATATCTAAACAAGGAGCCTTAAATTACATCTTTTTGGGATTACGTGGCG AGGGGGAGCAGCAGGGAGCATTTGCTTCTGGTAAAAGGATAGACCAGAAAGGACTTCCCCTTGGATGATC GGAGTCATGCAGAGCCCCCAGGGTCCCACATGTTCTGTGTGAACTTCATGTGGAATGACTCACAGAAGTG ACTACCTTAGGCGCTCGCTCTGTAGCTCAATGTGCGTAGAGTTTGTCTATTGTATAGGAAGCCCTGGGTT CTGTGCCCTGCCCCACACCATATAGGTATGATGGCGTGTACCCATATCCCAGCATTCAGGAAATCAAAGG TTGGCGGTCAAAAGTTCAAGGTGCAGCTATGTATCTTTTGAGATGTCCGAGTCTATAAGCTCCCTCTACT GAGAGCTGCTAGTATTCCTTCTCAAGAACAGGTTATTTGAAAATCTCTTCTTGTAAGCCCCAACATTTGG GGGAGGGGAGGATGGGGGAACTCTTGACTGAGCAGCTAGGTTTCCCCAAGCAGAAGTTCTTCTCTCCCAG TGCAAGCTGAGGTTTAGTGCCCCGGCTAGCCTCGGAACTGGCTCAGTGGGGGTTGGCACGCTGACTTCCT GGACAATTGCTGCATCTGACACTGTGAGCGCCCAGGACACCGGATGTGTAGCATGGATGTGGGCCTAGCA CAGGTGTGGAAGAGGACAGTCAGGACACTCACTGTTGGTGTTTGTTATCCACCTGTTCAGCCGCCGGGTC CCACACATAGGTCATCTTTCGTCACTCAAGCCGGCCTCTGTTTTTGATTTTAACAATCCAAGAGCAAGTG TGTAGGGGACAAAGAGACACCAGCTCTTCATACTTGATGGAGACCGGGACAGGATGCTGCAGGCAGGCTC GGGAGTGCATTATCGTTTCTTCTGAGCTCTTCATCCAGGCCATATTCCCTTTTCTGCATCGCATCTGGGG TGGGAGGGGCTGAGCTGGGAGTTTCCGTCCTTCTTCCCTGTGGGGGTGGGAGATGGGGCTCAAGGTCTCC ATCTCTCGGCTCCCTGAGGACAGAACCCCCACAGTGGACCTTTGGGCCTTCTCAGGACATTGACAATGTT GTGTGCACTGCAAGTTGACTTTATTTATTTTGTAGGAAAAAGAGATGGTATTCTTTAGCAGGATCTGAAA CTGTACCCTAGTCAAGAAGTACAATTAATAACATTATTATTAATAACAATTGGGATGATTCAAAGGTCAC ACACACACCAGACCACCCAAGAACCATGTAGGAGATGAGGCGGAGCCAGGTCATGGAAGCCCAGAATGCT ACAGAGATTCTGTGGGTCTATTATTGATCAGAAAATACAACATGGGGGGGCCTTTTCTATGGCATAACTC AGGTGTCTGCAAAGACAGAGCAGCTCAGGTGGGTGGATAGGCAGGCAGATGGAGCCTTGAATCACATCCC CTGAGGCTCAGCCAGCCAGTGCGAATCCTCAAGGTTCGGAAAGGGACAGTGTAGCGGCAAACTACTGGGC ATCTTCTCTGATGCTTAGAGGCTCTTACCAAGGAGCTTGATGGAGAAAGTGGCCATGTTGGTGGCATGGG ACAACTGCTCACCCAACTTCAAAGATCAAAGGGCACCCAGGTGGCCTATGACAGTGACACTCTTTCCTAC CATTAGGGGTGTCTGCCCCCACTGAAAGCCATGAAATTTCTGGCCCTAAGGGGGTAGGAAGGACTTAGGA GTAGCAGATGGTTTGATCCCATCCCCCCCACACACACACACACATCCCTCAGCTGTCTTCCACATTAGAG CCACTTCAGTTGTCCATGGACTTGTCCCTTTGAGACATCCTGGATTTTGAAGGATAGAAACATCCCCAAG ATGGTCTCGTGTTAATCCCACAACAGAGGCAGAAGGGTCATACTGAGAGAGAGAGAGAGAGAGAGAGAGA GAGAGAGAGGAGTGGGGGCCAGACCCTCAAGAAGCCAAGTGGGTCTGGTCAACCTGTGCACATGAGAAGG AGGGAACATCACTAAAATCAGGGCCTGGGCTGGTGTGTTGTTGGTGAGATCCCGTGGAGTGGCTGGCTAG ATATGGATGAGTTTTCTGAGCATGCTCACACACCCCCAACTTCACATTCTTAGAAATAGCACAACCATAA TGCCTTACCTCAAAAGGATGAGGCAAAGCTTGCAATTAATTCCCAGTGTCTGAAGACCTCCGGGATCCTT TGAGCTGTGTGTCCTTGTCAGTACATGGGGACAGGCTCCTTAGGTATTCCTGACATAGAGGTAAGGTGCT GCCCTTGCTGCCTTGCATAAGCTGTGACAAGCTTCTTACTGGGCACATAGAACGGCTCTGTCATCTGCTT CCACTAAATTTGGGCTTGGACTTTGCCTCCTGCCAAATCTCCATCTCTGCTGGATAGTCTAGTCCCTAGG GTCTAACCACCACCCTCCACTTCCTGGTGGGTCCTACAAGCGCTGTCTTTGTGCCAGTACCCGGATGGTG TCCTGCCTCATGGCTAAATGGTACAGGACATCTTCCCAGACTGAGTGGTCATGGCATGTGCATGTATGTT CATGAGTACATGCATGGTGTGTGGGGGTGGATGGGTGCATTCTGTTCTGCTTCTGGTGCAGCTACACAGC CACAACCTCTTTCTGTCATTGACCTTCTTGGTCTTCTTGTAGCCACAGATAATCTTCCAATGCCCGATTC TGCTGTTCTCATCTGAGAGCTGACAACCCAGCGCTCAGAGTAGAGTTCATGACCTAGGAAACCCTTCTCC TGGTAGCTTACTGAACTTATTTAATTAAAAACGAACATATAGGGGTTGGAGAGATGGTTCATTGGTTAAG AGCACCGGCTGCTTTTGCAAAGGACCTGAGTTCAAATCCCAGCAACCATATGGTGGCTTACAACCATCTG TAATGAGTTCTGGTGCCCTCTTCTGGCATGCAGGTGTACATGCAGATAGAGCACTCCTATGCATAAAATA AATAAATCTTTTAAATAAATAAATAAATGAACATGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_016803
• Insert Size: 1419 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055055, AAH55055
• RefSeq Size: 6000 bp
• RefSeq ORF: 1419 bp
• Locus ID: 53374
• UniProt ID: O88199
• Cytogenetics: 10 B4
• Gene Summary: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.[UniProtKB/Swiss-Prot Function]