Efcab3 (NM_001081046) Mouse Tagged ORF Clone

CAT#: MR206900

Efcab3 (Myc-DDK-tagged) - Mouse EF-hand calcium binding domain 3 (Efcab3)

Product Images

Circular map for MR206900

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Efcab3
• Synonyms: 4921510J17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206900 representing NM_001081046
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTTTCAGAAATTAAAGCCACGATGAAGCTAAAGCCCTTAAAAGCACCTGTCTTTCACAAGAGAG
  ATAAAGATTTGCGAGGATCTTTCTCAGACCATTTAAAGCACAAAGAATCAAAGCTGAGTCCTGCACAACT
  TGAAGCCTTCCGTAATGCTTACAACTTCTTCACCAAGGATAGAACTGGCTGTATTGATTCACACGGATTG
  ATAAGCACCATAGCAAAACTGGGAATGAATCTAAACACGTACGATATCTACAATGAATTAAAATGTGCTG
  ATCTCGACAGGGACGGTAAGATCAACTTCTCAGATTTTATAAATGTGTTGACAGATAAGAAACTCTTCCT
  CAAGGCTGTGGTTCCAGAAAAGAAAATATGCTTAGATTTAGCTAACAACCCAGGAATCTTGTTGTTTGAA
  ATCCTATCAAAATTTGTAGAAACCTCGTCCCTGCACAGAAAGGATATAATAGAGCTAGTAAGCTATTTCC
  GAAAAAGGTTCCAGGAGAGCCACTCAGAAATAATGTGGAGTTCGTATGGGAGAAGGGGCCTAAAGTCAGA
  GATCTGCTCTCCCCCTCGCTCGAGCACTGCTGCCTTTGCCAACTCTGCTAGGATCTCCATAATGAAAGAA
  AGGGATTTGTATAAGTTTCTCGAAGCCCTCAAACGGTGTAATCTCCGTACAGATAGCCCATACTCAAAAA
  TACCTGTGTTTCCGTTGTTTCCTGATGTGGATGGGACGGTGATGGGAAAACCATTTAAAGACACACAAAA
  AATAGAGATGCTAAGGAGAAAGGAGCCTCTGACTTTCTTTGAGGACTATTTTTTCAATAAAAGAGATTGG
  AAAACACAGGCCATGAATGTGAAACCTCTCAAGTCTGCCTCAGGCTATTCAGATGACATCTTAGCCATTG
  ACCACCTGTTCAAGAAAAAGCAACATTGGACTGTGACTGATGCGGCTGCTATCAAACAGCATGTAAAGAA
  AGCCACGGAGAGTTATAACTTAGGAATTGCCCTTGACCACCGGAAAGAAATGCTGAACCTCTGGAAGAAG
  ATTCGAGGGGATTTGGTTGGGATCGAAAGCAACAATGAGTCCTTTTACAACACCTTTTCGACCTACACCT
  GGTCCTGGAACGTCTGCCAAGAGCTGCTCTCCGCTAAGGACCTCCGCTTGCACGACGCCAGCATGAACAA
  GAGTTCCCCCTCCAACTCTGGGTTATCTTCCCCATCGGACTTCAGTGAGTCTGACCCGGAGACGGGGAGG
  AAGAGGAAACGGAAAAGTTCCAGAGGGTTTCGGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206900 representing NM_001081046
  Red=Cloning site Green=Tags(s)
  
  MAVSEIKATMKLKPLKAPVFHKRDKDLRGSFSDHLKHKESKLSPAQLEAFRNAYNFFTKDRTGCIDSHGL
  ISTIAKLGMNLNTYDIYNELKCADLDRDGKINFSDFINVLTDKKLFLKAVVPEKKICLDLANNPGILLFE
  ILSKFVETSSLHRKDIIELVSYFRKRFQESHSEIMWSSYGRRGLKSEICSPPRSSTAAFANSARISIMKE
  RDLYKFLEALKRCNLRTDSPYSKIPVFPLFPDVDGTVMGKPFKDTQKIEMLRRKEPLTFFEDYFFNKRDW
  KTQAMNVKPLKSASGYSDDILAIDHLFKKKQHWTVTDAAAIKQHVKKATESYNLGIALDHRKEMLNLWKK
  IRGDLVGIESNNESFYNTFSTYTWSWNVCQELLSAKDLRLHDASMNKSSPSNSGLSSPSDFSESDPETGR
  KRKRKSSRGFRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081046
• ORF Size: 1296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081046.1, NP_001074515.1
• RefSeq Size: 1744 bp
• RefSeq ORF: 1299 bp
• Locus ID: 70894
• UniProt ID: Q80X60
• Cytogenetics: 11 E1
• MW: 49.7 kDa