Kynu (BC069848) Mouse Tagged ORF Clone

CAT#: MR206825

Kynu (Myc-DDK-tagged) - Mouse kynureninase (L-kynurenine hydrolase) (cDNA clone MGC:78020 IMAGE:5097145)

Product Images

Circular map for MR206825

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kynu
• Synonyms: 4432411A05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206825 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGCCTTCGCCTCTTGAGCTTCCAGTTGATGCAGTGCGGCGCATCGCGGCTGAACTCAATTGTG
  ACCCAACAGATGAGAGGGTTGCTCTCCGCTTGGATGAGGAAGATAAACTGAGTCATTTTAGGAACTGTTT
  TTATATTCCCAAAATGCGGGACCTGCCTTCAATTGATCTATCTTTAGTGAGTGAGGATGATGATGCCATC
  TATTTCCTGGGAAATTCCCTTGGCCTTCAACCGAAAATGGTTAGGACATACCTGGAGGAAGAACTAGATA
  AGTGGGCCAAGATGGGAGCCTATGGCCATGATGTAGGCAAACGCCCTTGGATTGTAGGGGATGAGAGTAT
  TGTAAGCCTTATGAAGGACATTGTAGGAGCCCATGAGAAAGAAATAGCTCTAATGAATGCTTTGACTATT
  AATTTACATCTCCTGCTGTTATCATTCTTTAAGCCTACTCCAAAGCGGCACAAAATTCTTCTAGAAGCCA
  AAGCCTTCCCTTCTGATCATTATGCTATTGAGTCACAGATTCAACTTCACGGACTTGATGTTGAGAAAAG
  TATGCGGATGGTAAAGCCACGAGAGGGGGAAGAGACCTTAAGGATGGAGGACATACTGGAAGTAATCGAG
  GAGGAAGGAGACTCGATCGCCGTGATCCTGTTCAGTGGGCTGCACTTTTATACTGGACAGCTGTTCAACA
  TTCCTGCCATAACAAAAGCTGGACATGCAAAGGGCTGTTTTGTTGGCTTTGACCTAGCACATGCAGTTGG
  AAATGTTGAACTCCGCTTACATGACTGGGGTGTTGACTTTGCCTGCTGGTGTTCCTATAAGTATTTAAAT
  TCAGGAGCTGGAGGTCTGGCTGGTGCCTTTGTCCACGAGAAACATGCTCATACTGTCAAGCCTGCGTTAG
  TGGGATGGTTCGGCCATGACCTCAGTACAAGGTTTAACATGGATAACAAACTACAATTAATCCCCGGGGC
  CAATGGATTCCGAATTTCAAACCCTCCCATTTTGTTGGTCTGCTCCTTGCACGCCAGTTTAGAGGTCTTT
  CAGCAAGCAACTATGACTGCGCTGAGAAGAAAATCCATTCTGCTGACAGGTTATCTGGAATACATGCTCA
  AACATTACCACAGCAAAGATAACACCGAAAACAAGGGGCCGATTGTGAATATCATCACCCCGTCCAGAGC
  AGAGGAGCGTGGCTGCCAGTTAACACTCACCTTTTCCATTCCCAAGAAAAGCGTTTTTAAGGAACTAGAA
  AAAAGAGGAGTCGTTGTATGTATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206825 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMEPSPLELPVDAVRRIAAELNCDPTDERVALRLDEEDKLSHFRNCFYIPKMRDLPSIDLSLVSEDDDAI
  YFLGNSLGLQPKMVRTYLEEELDKWAKMGAYGHDVGKRPWIVGDESIVSLMKDIVGAHEKEIALMNALTI
  NLHLLLLSFFKPTPKRHKILLEAKAFPSDHYAIESQIQLHGLDVEKSMRMVKPREGEETLRMEDILEVIE
  EEGDSIAVILFSGLHFYTGQLFNIPAITKAGHAKGCFVGFDLAHAVGNVELRLHDWGVDFACWCSYKYLN
  SGAGGLAGAFVHEKHAHTVKPALVGWFGHDLSTRFNMDNKLQLIPGANGFRISNPPILLVCSLHASLEVF
  QQATMTALRRKSILLTGYLEYMLKHYHSKDNTENKGPIVNIITPSRAEERGCQLTLTFSIPKKSVFKELE
  KRGVVVCI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC069848
• ORF Size: 1284 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC069848, AAH69848
• RefSeq Size: 1599 bp
• RefSeq ORF: 1286 bp
• Locus ID: 70789
• Cytogenetics: 2 B
• MW: 48.2 kDa
• Gene Summary: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.[UniProtKB/Swiss-Prot Function]