Pisd (NM_177298) Mouse Tagged ORF Clone

CAT#: MR206380

Pisd (Myc-DDK-tagged) - Mouse phosphatidylserine decarboxylase (Pisd)

Product Images

Circular map for MR206380

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pisd
• Synonyms: 9030221M09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206380 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGTCTGGGGGCCGCGCGTGTGTGCGCTCGCTTCGTGGAGGTGTGCTGTGGCGGAGCAGCCCCT
  GTCACTATGAATCTACTGCCACACGCCATTTTCTGGGGACCTTACAGAAATTGCCTCTTCAGGCAGGTGT
  CAGAAATTTCCATACTGCTCCTGTCCGATCCTTGTTCCTGCTGCGCCCTGTGCCCATCCTGTTGGCAACA
  GGTGGAGGGTATGCCGGGTACCGGCAGTATGAGAAGTACAGGGAACGGAAGCTTGAGAAGCTGGGCTTGG
  AGATCCCGCCCAAACTTGCTAGTCACTGGGAGGTGTCCCTGTATAAATCTGTGCCAACGCGTTTGCTGTC
  ACGTGCCTGTGGTCGCCTCAACCAAGTAGAACTTCCTTACTGGCTCCGGAGGCCAGTCTACAGCCTGTAT
  ATCTGGACCTTTGGGGTGAACATGACTGAGGCTGCAGTGGAGGACCTGCACCACTACCGCAACCTCAGCG
  AGTTCTTCCGGCGTAAGCTGAAGCCTCAGGCACGGCCCGTATGTGGCTTGCACTGTGTGACCAGCCCATC
  AGATGGCAAGATCCTCACCTTTGGGCAGGTGAAGAACTCTGAGGTGGAGCAGGTAAAGGGCGTAACCTAC
  TCCTTGGAGTCCTTCCTGGGCCCTCGAGCCAATACAGAGGACCTGCCCTTCCCTCCAGCTTCCTCCAGTG
  ACTCCTTCAGAAACCAGCTGGTCACCCGGGAAGGGAATGAGCTGTACCACTGTGTCATCTACCTGGCCCC
  AGGAGACTACCACTGCTTCCACTCCCCCACTGACTGGACCATCTCACATCGGCGCCACTTCCCAGGTTCC
  CTGATGTCAGTGAACCCTGGCATGGCCCGATGGATCAAAGAGCTCTTTTGTCACAATGAGCGTGTAGTCC
  TAACTGGAGACTGGAAACATGGGTTCTTCTCGTTGACAGCAGTGGGAGCCACCAATGTGGGCTCTATCCG
  TATCCACTTTGACCGAGACCTGCACACAAACAGCCCCAGGTACAGCAAGGGCTCCTACAATGACCTGAGC
  TTTGTAACACATGCCAACAAGGAGGGCATTCCCATGCGCAAGGGTGAGCCCCTGGGGGAGTTCAACCTGG
  GCTCCACCATCGTGCTCATCTTCGAGGCACCCAAGGACTTCAACTTCAGGCTGAAGGCAGGGCAGAAGAT
  CCGCTTTGGGGAAGCACTGGGCTCCCTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206380 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASGGRACVRSLRGGVLWRSSPCHYESTATRHFLGTLQKLPLQAGVRNFHTAPVRSLFLLRPVPILLAT
  GGGYAGYRQYEKYRERKLEKLGLEIPPKLASHWEVSLYKSVPTRLLSRACGRLNQVELPYWLRRPVYSLY
  IWTFGVNMTEAAVEDLHHYRNLSEFFRRKLKPQARPVCGLHCVTSPSDGKILTFGQVKNSEVEQVKGVTY
  SLESFLGPRANTEDLPFPPASSSDSFRNQLVTREGNELYHCVIYLAPGDYHCFHSPTDWTISHRRHFPGS
  LMSVNPGMARWIKELFCHNERVVLTGDWKHGFFSLTAVGATNVGSIRIHFDRDLHTNSPRYSKGSYNDLS
  FVTHANKEGIPMRKGEPLGEFNLGSTIVLIFEAPKDFNFRLKAGQKIRFGEALGSL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177298
• ORF Size: 1221 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177298.3, NP_796272.2
• RefSeq Size: 2150 bp
• RefSeq ORF: 1221 bp
• Locus ID: 320951
• UniProt ID: Q8BSF4
• Cytogenetics: 5 B1
• MW: 45.9 kDa
• Gene Summary: Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.[UniProtKB/Swiss-Prot Function]