Dxo (NM_033613) Mouse Tagged ORF Clone

CAT#: MR206225

Dom3z (Myc-DDK-tagged) - Mouse DOM-3 homolog Z (C. elegans) (Dom3z), transcript variant 1

Product Images

Circular map for MR206225

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dxo
• Synonyms: Dom3z; NG6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206225 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCCAGAGGGACTAAGAGAAAAGCGGAGAAGACAGAGGTGGAAAAGCCTTTGAACAAACTCCCCC
  GGGCAGTCCCTTCACTGCGGACACAGCCCTCTCTGTACTCGGGACCCTTCCCGTTCTACCGGCGTCCATC
  CGAACTGGGCTGCTTCTCCCTGGACGCACAACGCCAGTACCATGGAGATGCCCGAGCCCTCCGCTACTAC
  AGCCCCCCTCCCATCAACGGCCCAGGCCCGGACTTTGACCTCAGAGATGGATACCCTGACCGATACCAGC
  CCCGGGACGAGGAAGTCCAAGAGAGGTTGGACCATTTGCTGCGCTGGGTCCTGGAACACCGGAACCAGCT
  GGAGGGGGGTCCCGGCTGGCTGGCAGGGGCCACGGTGACATGGAGAGGGCACCTGACAAAATTGCTGACC
  ACGCCGTATGAGCGGCAGGAGGGCTGGCAGCTGGCAGCCTCCCGGTTCCAAGGGACACTGTACCTAAGTG
  AGGTGGAGACCCCGGCTGCTCGGGCCCAGAGGCTTGCACGGCCACCCCTCCTCCGGGAGCTCATGTACAT
  GGGCTACAAATTTGAGCAGTACATGTGCGCAGACAAACCCGGAGGCTCCCCAGACCCCTCTGGGGAAGTT
  AACACCAACGTGGCCTACTGCTCTGTGCTACGCAGCCGCCTGGGAAACCACCCTCTCCTGTTCTCCGGGG
  AGGTAGACTGCCTGAACCCCCAGGCTCCTTGCACACAGCCTCCCTCCTGCTACGTGGAGCTCAAGACATC
  CAAGGAGATGCACAGCCCTGGCCAGTGGAGGAGCTTTTACAGACACAAGTTGCTGAAATGGTGGGCTCAG
  TCGTTCCTCCCGGGGGTCCCACATGTTGTTGCCGGCTTCCGGAACCCAGAAGGTTTCGTCTGTTCCTTAA
  AGACCTTTCCTACCATGGAGATGTTTGAAAATGTCAGGAATGACCGAGAAGGCTGGAATCCCTCTGTGTG
  CATGAACTTCTGTGCTGCCTTCCTTAGCTTTGCCCAGAGCACAGTAGTCCAGGACGATCCCAGGCTTGTC
  CACCTCTTCTCCTGGGAACCTGGTGGCCCTGTCACTGTGTCTGTCCATCGTGACGCACCCTATGCCTTCC
  TGCCCTCATGGTATGTGGAGACTATGACCCAAGACCTCCCACCACTCTCAAAGACTCCCTCTCCCAAGGA
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206225 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPRGTKRKAEKTEVEKPLNKLPRAVPSLRTQPSLYSGPFPFYRRPSELGCFSLDAQRQYHGDARALRYY
  SPPPINGPGPDFDLRDGYPDRYQPRDEEVQERLDHLLRWVLEHRNQLEGGPGWLAGATVTWRGHLTKLLT
  TPYERQEGWQLAASRFQGTLYLSEVETPAARAQRLARPPLLRELMYMGYKFEQYMCADKPGGSPDPSGEV
  NTNVAYCSVLRSRLGNHPLLFSGEVDCLNPQAPCTQPPSCYVELKTSKEMHSPGQWRSFYRHKLLKWWAQ
  SFLPGVPHVVAGFRNPEGFVCSLKTFPTMEMFENVRNDREGWNPSVCMNFCAAFLSFAQSTVVQDDPRLV
  HLFSWEPGGPVTVSVHRDAPYAFLPSWYVETMTQDLPPLSKTPSPKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033613
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033613.1, NP_291091.1
• RefSeq Size: 1472 bp
• RefSeq ORF: 1194 bp
• Locus ID: 112403
• UniProt ID: O70348
• Cytogenetics: 17 B1
• MW: 45.3 kDa
• Gene Summary: Ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap and is part of a pre-mRNA capping quality control. Has decapping, pyrophosphohydrolase and 5'-3' exonuclease activities. Has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN and 5' end monophosphate RNA. The 5' end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates.[UniProtKB/Swiss-Prot Function]