Rorb (BC024842) Mouse Tagged ORF Clone

CAT#: MR206022

Rorb (Myc-DDK-tagged) - Mouse RAR-related orphan receptor beta (cDNA clone MGC:38728 IMAGE:5358124)

Product Images

Circular map for MR206022

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rorb
• Synonyms: MGC38728, Nr1f2, RZR-beta, RZRB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206022 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAGAGATGCTGTAAAGTTCGGGAGGATGTCCAAGAAGCAGCGGGACAGCCTGTATGCTGAGGTGC
  AGAAGCATCAGCAAAGGCTGCAGGAGCAGCGGCAGCAGCAGAGTGGGGAGGCGGAGGCCCTCGCCAGGGT
  GTACAGCAGCAGCATTAGCAATGGCCTCAGCAACCTGAACACCGAGACCGGCGGCACATACGCCAACGGG
  CACGTCATTGACCTGCCCAAGTCCGAAGGTTATTACAGCATAGATTCCGGTCAGCCGTCTCCCGATCAGT
  CAGGACTGGACATGACTGGGATCAAACAGATAAAGCAAGAACCTATCTATGACCTCACATCTGTACCCAA
  CTTGTTTACCTATAGCTCTTTCAACAACGGGCAGTTAGCTCCCGGGATAACAATGTCTGAGATCGATCGA
  ATTGCACAGAACATCATTAAGTCCCATTTGGAGACATGTCAGTACACCATGGAAGAACTCCATCAGCTGG
  CATGGCAGACCCACACCTACGAGGAAATCAAGGCGTATCAAAGCAAGTCCAGGGAGGCTCTGTGGCAGCA
  GTGTGCCATCCAGATCACCCATGCTATCCAGTACGTGGTGGAGTTCGCCAAGCGGATAACAGGCTTCATG
  GAGCTGTGTCAGAACGATCAGATCTTACTTCTGAAGTCAGGTTGCTTGGAAGTGGTTTTAGTGAGAATGT
  GTCGTGCCTTCAACCCATTAAACAACACTGTTCTGTTTGAAGGAAAATATGGAGGAATGCAAATGTTCAA
  AGCCTTAGGTTCGGATGACCTAGTGAATGAAGCATTTGACTTTGCGAAGAATCTGTGTTCCTTGCAGCTG
  ACTGAGGAAGAGATTGCTCTGTTCTCCTCTGCTGTTCTGATATCCCCAGACCGAGCCTGGCTGATCGAAC
  CAAGAAAAGTCCAGAAGCTTCAGGAAAAGATTTATTTTGCACTGCAACATGTGATTCAGAAGAACCACCT
  GGATGATGAGACCCTGGCAAAGTTAATAGCCAAGATACCAACTATCACGGCAGTCTGCAACTTGCATGGG
  GAGAAGCTGCAGGTATTTAAGCAGTCTCATCCAGACATAGTGAATACACTGTTTCCTCCATTGTACAAGG
  AGCTCTTTAATCCTGACTGTGCTGCGGTCTGCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206022 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRDAVKFGRMSKKQRDSLYAEVQKHQQRLQEQRQQQSGEAEALARVYSSSISNGLSNLNTETGGTYANG
  HVIDLPKSEGYYSIDSGQPSPDQSGLDMTGIKQIKQEPIYDLTSVPNLFTYSSFNNGQLAPGITMSEIDR
  IAQNIIKSHLETCQYTMEELHQLAWQTHTYEEIKAYQSKSREALWQQCAIQITHAIQYVVEFAKRITGFM
  ELCQNDQILLLKSGCLEVVLVRMCRAFNPLNNTVLFEGKYGGMQMFKALGSDDLVNEAFDFAKNLCSLQL
  TEEEIALFSSAVLISPDRAWLIEPRKVQKLQEKIYFALQHVIQKNHLDDETLAKLIAKIPTITAVCNLHG
  EKLQVFKQSHPDIVNTLFPPLYKELFNPDCAAVCK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC024842
• ORF Size: 1155 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024842, AAH24842
• RefSeq Size: 1832 bp
• RefSeq ORF: 1157 bp
• Locus ID: 225998
• Cytogenetics: 19 B
• MW: 43.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It is a DNA-binding protein that can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, and to help regulate the expression of some genes involved in circadian rhythm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]