Hmbs (NM_001110251) Mouse Tagged ORF Clone

CAT#: MR205538

Hmbs (Myc-DDK-tagged) - Mouse hydroxymethylbilane synthase (Hmbs), transcript variant 2

Product Images

Circular map for MR205538

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hmbs
• Synonyms: PBGD; T25658; Ups; Uros1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205538 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGTAACGGCGGCGCGGCCACAACCGCGGAAGAAAACGGCTCAAAGATGAGGGTGATTCGAGTGG
  GCACCCGTAAGAGCCAGCTGGCTCGCATACAGACCGACACTGTGGTGGCGATGCTGAAAGCCTTGTACCC
  TGGCATACAGTTTGAAATCATTGCTATGTCCACCACGGGAGACAAGATTCTTGATACTGCACTCTCTAAG
  ATTGGAGAGAAGAGCCTGTTTACCAAGGAGCTAGAAAACGCCCTGGAAAAAAACGAAGTGGACCTGGTTG
  TTCACTCCCTGAAGGATGTGCCTACCATACTACCTCCTGGCTTTACTATTGGAGCCATCTGCAAACGGGA
  AAACCCTTGTGATGCTGTTGTCTTTCACCCAAAGTTTATTGGAAAGACCCTGGAAACCTTGCCAGAGAAA
  AGTGCCGTGGGAACCAGCTCTCTGAGGAGAGTGGCTCAGCTACAGAGAAAGTTCCCCAACCTGGAATTCA
  AGAGTATTCGGGGAAACCTCAACACCCGCCTTCGGAAGCTGGATGAGCTGCAGGAATTCAGTGCCATTGT
  CCTGGCTGTGGCTGGCCTACAGCGCATGGGCTGGCAGAACCGGGTGGGCCAGATTTTGCACCCAGAGGAA
  TGCATGTATGCTGTGGGTCAGGGGGCCCTAGCCGTGGAAGTCCGAGCCAAGGACCAGGATATCTTGGACC
  TAGTGAGTGTGTTGCACGATCCTGAAACTCTGCTTCGCTGCATTGCTGAAAGGGCTTTTCTGAGGCACCT
  GGAAGGAGGCTGCAGCGTGCCCGTAGCAGTGCATACAGTGATGAAAGATGGGCAACTGTACCTGACTGGT
  GGAGTATGGAGTCTAGATGGCTCAGATAGCATGCAAGAGACTATGCAGGCCACCATCCAGGTCCCTGTTC
  AGCAAGAAGATGGTCCAGAAGATGACCCACAACTGGTTGGAATCACTGCCCGGAACATTCCAAGAGGAGC
  CCAGCTAGCTGCTGAGAACCTGGGCATCAGCCTGGCCAGCTTGCTGCTCAACAAAGGAGCCAAGAACATC
  CTGGATGTTGCACGGCAGCTTAATGATGTGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205538 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGNGGAATTAEENGSKMRVIRVGTRKSQLARIQTDTVVAMLKALYPGIQFEIIAMSTTGDKILDTALSK
  IGEKSLFTKELENALEKNEVDLVVHSLKDVPTILPPGFTIGAICKRENPCDAVVFHPKFIGKTLETLPEK
  SAVGTSSLRRVAQLQRKFPNLEFKSIRGNLNTRLRKLDELQEFSAIVLAVAGLQRMGWQNRVGQILHPEE
  CMYAVGQGALAVEVRAKDQDILDLVSVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTVMKDGQLYLTG
  GVWSLDGSDSMQETMQATIQVPVQQEDGPEDDPQLVGITARNIPRGAQLAAENLGISLASLLLNKGAKNI
  LDVARQLNDVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110251
• ORF Size: 1086 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1553 bp
• RefSeq ORF: 1035 bp
• Locus ID: 15288
• Cytogenetics: 9 24.84 cM
• MW: 39.3 kDa
• Gene Summary: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.[UniProtKB/Swiss-Prot Function]