Cdk10 (NM_194444) Mouse Tagged ORF Clone

CAT#: MR204870

Cdk10 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 10 (Cdk10), transcript variant 2

Product Images

Circular map for MR204870

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdk10
• Synonyms: BC017131
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204870 representing NM_194444
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGGAAGATGCCGAAGCGTTAAGGAGTTTGAGAAGCTGAACCGGATTGGCGAGGGCACCTATGGCATCG
  TGTATCGGGCCAGGGATACCCAGACAGATGAAATTGTCGCCCTGAAGAAGGTGCGGATGGACAAAGAGAA
  GGATGGCATCCCCATCAGCAGCCTGCGTGAGATCACACTGCTCTTGCGTCTCCGCCATCCAAACATTGTG
  GAGCTGAAGGAGGTGGTTGTGGGCAACCACCTGGAGAGCATCTTCCTGGTCATGGGTTACTGCGAACAAG
  ATCTGGCCAGCCTATTGGAAAATATGCCAACACCCTTCTCGGAGGCCCAGGTTAAATGCATCATGCTACA
  GGTGCTTCGTGGCCTTCAGTACCTGCACAGGAACTTCATCATCCACAGGGACCTGAAGGTGTCCAACTTG
  CTCATGACAGACAAGGGCTGTGTAAAGACAGCTGATTTTGGCCTGGCTCGGGCCTATGGTGTTCCAGTAA
  AGCCAATGACTCCCAAGGTTGTTACCCTCTGGTACCGAGCCCCAGAGCTGCTGCTTGGAACTACCACCCA
  GACTACCAGCATTGACATGTGGGCTGTCGGCTGCATCCTGGCAGAGCTGCTGGCCCATAAGCCCCTCCTC
  CCTGGCACTTCCGAGATCCACCAGATCGACTTGATTGTACAGCTGTTGGGGACACCGAGTGAGAATATCT
  GGCCGGGTTTCTCCAAGCTGCCGCTGGCCGGCCAGTACAGCTTGAGGAAACAGCCCTATAACAACCTCAA
  GCACAAGTTCCCGTGGCTCTCAGAGGCCGGACTCCGTCTGCTCAACTTCCTCTTCATGTATGACCCTAAG
  AAAAGGGCAACCTCGGGAGACTGCCTGGAGAGCTCCTACTTCAAGGAGAAGCCCCTGCCCTGTGAACCGG
  AGCTCATGCCTACCTTCCCCCACCACCGCAATAAGCGTGCTGCCCCAGCTGCCGCTGAAGGGCAGAGCAA
  ACGATGCCGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204870 representing NM_194444
  Red=Cloning site Green=Tags(s)
  
  LGRCRSVKEFEKLNRIGEGTYGIVYRARDTQTDEIVALKKVRMDKEKDGIPISSLREITLLLRLRHPNIV
  ELKEVVVGNHLESIFLVMGYCEQDLASLLENMPTPFSEAQVKCIMLQVLRGLQYLHRNFIIHRDLKVSNL
  LMTDKGCVKTADFGLARAYGVPVKPMTPKVVTLWYRAPELLLGTTTQTTSIDMWAVGCILAELLAHKPLL
  PGTSEIHQIDLIVQLLGTPSENIWPGFSKLPLAGQYSLRKQPYNNLKHKFPWLSEAGLRLLNFLFMYDPK
  KRATSGDCLESSYFKEKPLPCEPELMPTFPHHRNKRAAPAAAEGQSKRCRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194444
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194444.1
• RefSeq Size: 1623 bp
• RefSeq ORF: 870 bp
• Locus ID: 234854
• UniProt ID: Q3UMM4
• Cytogenetics: 8 E1
• MW: 33.1 kDa
• Gene Summary: The protein encoded by this gene belongs to the CDK (cyclin-dependent kinase) subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. The human ortholog has been shown to play a role in cellular proliferation. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 1. [provided by RefSeq, Jan 2010]