Phf23 (BC033533) Mouse Tagged ORF Clone
CAT#: MR204847
- TrueORF®
Phf23 (Myc-DDK-tagged) - Mouse PHD finger protein 23 (cDNA clone MGC:25514 IMAGE:2655211)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"BC033533" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Phf23 |
Synonyms | JUNE-1, RP23-172M21.13 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204847 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCCAGTCCCGAAGATCCGCCTCCGACTCTTAAACCAGAGACTCAGCCACCGGAGAAAAGGC GGAGAACCATTGAGGATTTTAACAAATTCTGCAGTTTTGTCTTGGCCTATGCTGGTTACATCCCCCCTAG CAAAGAGGCTCCTGACAGTGCTACTCTGCTTGAGAAGATGAAGCTCAAGGACTCTCTTTTTGATCTGGAT GGGCCCAAAGTGGCATCTCCACTCTCTCCCACATCATTGACGCATACCTCCCGGCCCCCCGCTGCTCTCG CCCCAGTGCCGCTGTCCCAGGGGGACCTCTCCCAGCCTCGAAAGAAGGACCGAAAGAATAGAAAGTTGGG ACCAGGAGGTGGGGCTGGCTTTGGGGTGCTTCGGAGACCTCGACCAGCTCCTGGGGATGGGGAAAAGCGG TCTCGAATCAAGAAGAGCAAGAAGCGGAAGTTGAAAAAGGCAGATCGGGGAGATAGACTCCCACCTCCTG GCCCTCCTAGGGCTCCTCCCAGTGATACAGACTCTGAAGAGGAGGAGGAAGAGGAAGAAGAGGAAGATGA TGAGGAAGAGATGACAGTGGGGGGTGGAGTCCCAGCCCCTGTGCTCCCAACACCCCCTGAGGCCCCCAGG CCCCCTGTTACAGTGCACTCTGAAGGTGCTCCCCCTACTGACAGTGAAGGCAAAGATGTGGGCAGCACAG AAACAAGCCAAGATGGAGATGCTAGCTCCAGTGAAGGCGAGATGCGGGTCATGGATGAGGACATCATGGT AGAATCAGGTGACGACTCCTGGGATCTGATCACGTGTTACTGCAGAAAGCCCTTTGCGGGGCGGCCCATG ATTGAGTGCAGCCTCTGTGGGACGTGGATCCACCTCTCCTGTGCTAAGATTAAGAAGACCAACGTTCCCG ACTTCTTTTATTGCCAGAAATGCAAGGAACTTCGGCCAGAGGCCCGGCGGTTAGGGGGTCTCCCCAAATC TGGAGAACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204847 protein sequence
Red=Cloning site Green=Tags(s) MAEPSPEDPPPTLKPETQPPEKRRRTIEDFNKFCSFVLAYAGYIPPSKEAPDSATLLEKMKLKDSLFDLD GPKVASPLSPTSLTHTSRPPAALAPVPLSQGDLSQPRKKDRKNRKLGPGGGAGFGVLRRPRPAPGDGEKR SRIKKSKKRKLKKADRGDRLPPPGPPRAPPSDTDSEEEEEEEEEEDDEEEMTVGGGVPAPVLPTPPEAPR PPVTVHSEGAPPTDSEGKDVGSTETSQDGDASSSEGEMRVMDEDIMVESGDDSWDLITCYCRKPFAGRPM IECSLCGTWIHLSCAKIKKTNVPDFFYCQKCKELRPEARRLGGLPKSGEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC033533 |
ORF Size | 990 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC033533, AAH33533 |
RefSeq Size | 1728 bp |
RefSeq ORF | 992 bp |
Locus ID | 78246 |
Cytogenetics | 11 |
MW | 36 kDa |
Gene Summary | Acts as a negative regulator of autophagy, through promoting ubiquitination and degradation of LRSAM1, an E3 ubiquitin ligase that promotes autophagy in response to starvation or infecting bacteria.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218494 | Phf23 (untagged) - Mouse PHD finger protein 23 (cDNA clone MGC:25514 IMAGE:2655211), (10ug) |
USD 330.00 |
|
MG204847 | Phf23 (tGFP-tagged) - Mouse PHD finger protein 23 (cDNA clone MGC:25514 IMAGE:2655211) |
USD 500.00 |
|
MR204847L3 | Lenti ORF clone of Phf23 (Myc-DDK-tagged) - Mouse PHD finger protein 23 (cDNA clone MGC:25514 IMAGE:2655211) |
USD 600.00 |
|
MR204847L4 | Lenti ORF clone of Phf23 (mGFP-tagged) - Mouse PHD finger protein 23 (cDNA clone MGC:25514 IMAGE:2655211) |
USD 600.00 |
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