Sike1 (NM_025679) Mouse Tagged ORF Clone
CAT#: MR202191
- TrueORF®
Sike1 (Myc-DDK-tagged) - Mouse suppressor of IKBKE 1 (Sike1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_025679" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Sike1 |
Synonyms | 2810005O12Rik; 5730470L24Rik; AI450236; AI839862; Sike; Sikeb |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202191 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTGCACGATCGAGAAGATCTTGACGGACGCCAAGACGCTGCTGGAGCGGCTGCGCGAGCACGATG CGGCGGCCGAGTCGCTGGTGGACCAGTCGGCTGCGCTGCACCGGCGGGTGGCGGCGATGCGGGAGGCGGG CGCCGTGTTACCCGAGCAGTATCAAGAGGATGCATCCGACGTGAAGGACATGTCTAAATATAAACCTCAC ATTCTGCTGTCTCAAGAGAATACCCAGATTAGAGACTTACAGCAGGAAAACAGAGAACTGTGGGTTTCCT TGGAGGAGCACCAGGATGCGTTGGAACTGATCATGAGCAAGTACCGGAAACAGATGTTACAGTTAATGGT CGCTAAGAAAGCCGTGGATGCCGAACCAGTTCTGAAAGCTCACCAGTCTCACTCTGCAGAGATTGAAAGT CAGATCGATAGAATCTGTGAAATGGGAGCAGTGATGCGGAGAGCTGTTCAGGTGGACGATAACCAATTCT GTAAGGTTCAGGAGAGACTAGCTCAGCTGGAGCTTGAAAATAAGGAACTTCGAGAGTTACTGTCCATCAG CAGTGAATCTCTGCAGGTTGGAAAAGAAAGCTCTGTGGCTCCTGCCTCTCAGACCATCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202191 protein sequence
Red=Cloning site Green=Tags(s) MSCTIEKILTDAKTLLERLREHDAAAESLVDQSAALHRRVAAMREAGAVLPEQYQEDASDVKDMSKYKPH ILLSQENTQIRDLQQENRELWVSLEEHQDALELIMSKYRKQMLQLMVAKKAVDAEPVLKAHQSHSAEIES QIDRICEMGAVMRRAVQVDDNQFCKVQERLAQLELENKELRELLSISSESLQVGKESSVAPASQTIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025679 |
ORF Size | 624 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025679.3 |
RefSeq Size | 2798 bp |
RefSeq ORF | 624 bp |
Locus ID | 66641 |
UniProt ID | Q9CPR7 |
Cytogenetics | 3 F2.2 |
MW | 23.5 kDa |
Gene Summary | Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200385 | Sike1 (untagged) - Mouse suppressor of IKBKE 1 (Sike1), (10ug) |
USD 300.00 |
|
MG202191 | Sike1 (tGFP-tagged) - Mouse RIKEN cDNA 5730470L24 gene (5730470L24Rik) |
USD 500.00 |
|
MR202191L3 | Lenti ORF clone of Sike1 (Myc-DDK-tagged) - Mouse suppressor of IKBKE 1 (Sike1) |
USD 600.00 |
|
MR202191L4 | Lenti ORF clone of Sike1 (mGFP-tagged) - Mouse suppressor of IKBKE 1 (Sike1) |
USD 600.00 |
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