Rab30 (NM_029494) Mouse Tagged ORF Clone
CAT#: MR202099
- TrueORF®
Rab30 (Myc-DDK-tagged) - Mouse RAB30, member RAS oncogene family (Rab30)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_029494" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Rab30 |
Synonyms | 5033421K01Rik; AI323892; Rsb30 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202099 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTATGGAAGATTATGATTTCCTGTTCAAAATTGTTTTAATTGGCAACGCTGGAGTGGGAAAGACGT GCCTAGTCCGAAGATTCACTCAGGGTCTTTTCCCCCCAGGTCAAGGAGCCACAATTGGAGTTGATTTTAT GATTAAGACAGTGGAGATTAATGGTGAAAAAGTGAAGTTACAGATCTGGGACACCGCAGGTCAAGAGAGA TTTCGCTCCATCACTCAAAGTTACTATCGAAGCGCCAATGCCTTGATCCTTACCTATGACATCACCTGTG AGGAATCCTTCCGCTGCCTTCCTGAGTGGTTGCGGGAGATAGAACAGTATGCTAGCAATAAAGTCATCAC TGTGTTAGTAGGCAACAAGATTGACCTGGCTGAAAGGCGAGAGGTCTCCCAGCAGAGAGCAGAAGAGTTC TCAGAGGCTCAGGACATGTATTACCTGGAGACTTCAGCCAAGGAATCCGACAATGTGGAGAAACTCTTCC TTGACTTAGCATGCCGACTCATCAGCGAAGCAAGACAGAACACACTGGTGAACAATGTATCGTCACCCTT ACCCGGAGAGGGGAAAAGCATCAGCTATTTGACTTGTTGTAATTTCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202099 protein sequence
Red=Cloning site Green=Tags(s) MSMEDYDFLFKIVLIGNAGVGKTCLVRRFTQGLFPPGQGATIGVDFMIKTVEINGEKVKLQIWDTAGQER FRSITQSYYRSANALILTYDITCEESFRCLPEWLREIEQYASNKVITVLVGNKIDLAERREVSQQRAEEF SEAQDMYYLETSAKESDNVEKLFLDLACRLISEARQNTLVNNVSSPLPGEGKSISYLTCCNFN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_029494 |
ORF Size | 612 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_029494.2, NP_083770.2 |
RefSeq Size | 2007 bp |
RefSeq ORF | 612 bp |
Locus ID | 75985 |
UniProt ID | Q923S9 |
Cytogenetics | 7 E1 |
MW | 23.1 kDa |
Gene Summary | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). Required for maintaining the structural integrity of the Golgi apparatus, possibly by mediating interactions with cytoplasmic scaffolding proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203956 | Rab30 (untagged) - Mouse RAB30, member RAS oncogene family (Rab30), (10ug) |
USD 300.00 |
|
MG202099 | Rab30 (tGFP-tagged) - Mouse RAB30, member RAS oncogene family (Rab30) |
USD 500.00 |
|
MR202099L3 | Lenti ORF clone of Rab30 (Myc-DDK-tagged) - Mouse RAB30, member RAS oncogene family (Rab30) |
USD 600.00 |
|
MR202099L4 | Lenti ORF clone of Rab30 (mGFP-tagged) - Mouse RAB30, member RAS oncogene family (Rab30) |
USD 600.00 |
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