Eaf2 (NM_134111) Mouse Tagged ORF Clone

CAT#: MR200759

Eaf2 (Myc-DDK-tagged) - Mouse ELL associated factor 2 (Eaf2), transcript variant 1

Product Images

Circular map for MR200759

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eaf2
• Synonyms: AW048865; Festa; FESTA-L; FESTA-S; Traits; U19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR200759 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAATCTGCCTAGGACTTCCAATCTTGTACAGCATTCTCCATCAGAAGAGAAGATGTCTCCAACGT
  CTCTAATGGATGATATTGAAAGAGAACTGAAAGCAGAAGCTAGTCTTATGGACCAGATGAGTAGTTGTGA
  TAGTTCATCAGATTCCAAAAGTTCTTCATCTTCAAGTAGTGAGGATAGTTCTAGTGATTCTGAAGATGAT
  GACCAATTCTCTCCTTTGGGTCCAAGGAAATACAGCTCGGAGCACCCTAGCATGTCTGCTGGGCCACAGT
  ACAGGACTTCAGAGGCTGATGCTACTTGTCACCGACTTCAGGACCACAGTACCCTTCTGATGAGTACTTT
  ACGAAGTGACTTGCAGCTGAGTGAGTCAGAAAGCGACAGTGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR200759 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWNLPRTSNLVQHSPSEEKMSPTSLMDDIERELKAEASLMDQMSSCDSSSDSKSSSSSSSEDSSSDSEDD
  DQFSPLGPRKYSSEHPSMSAGPQYRTSEADATCHRLQDHSTLLMSTLRSDLQLSESESDSED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134111
• ORF Size: 399 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134111.2, NP_598872.1
• RefSeq Size: 1903 bp
• RefSeq ORF: 399 bp
• Locus ID: 106389
• UniProt ID: Q91ZD6
• Cytogenetics: 16 B3
• MW: 14.5 kDa
• Gene Summary: Acts as a transcriptional transactivator of ELL and ELL2 elongation activities (By similarity). Acts as a transcriptional transactivator of TCEA1 elongation activity.[UniProtKB/Swiss-Prot Function]