Abcb1b (NM_011075) Mouse Tagged ORF Clone
CAT#: MG227343
- TrueORF®
Abcb1b (tGFP-tagged) - Mouse ATP-binding cassette sub-family B (MDR/TAP) member 1B (Abcb1b), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_011075" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Abcb1b |
Synonyms | Abcb1; mdr; Mdr1; Mdr1b; Pgy-1; Pgy1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227343 representing NM_011075
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTTGAAGAGAACCTTAAGGGAAGAGCAGACAAGAACTTCTCGAAGATGGGCAAAAAGAGTAAAA AGGAGAAGAAAGAAAAGAAACCTGCTGTTGGCGTATTTGGGATGTTTCGCTATGCAGATTGGCTGGACAA GCTGTGCATGATTCTGGGAACTCTCGCTGCTATTATCCATGGAACATTACTTCCCCTCTTGATGCTGGTG TTTGGAAACATGACAGATAGTTTTACAAAAGCAGAAGCCAGTATTCTGCCAAGCATTACTAATCAAAGTG GACCCAACAGTACTCTGATCATCAGCAACAGCAGTCTGGAGGAAGAGATGGCCATATACGCCTACTATTA CACCGGGATTGGTGCTGGTGTGCTCATAGTTGCCTACATCCAGGTTTCACTTTGGTGCCTGGCAGCTGGA AGACAGATACACAAGATTAGGCAGAAGTTTTTCCATGCTATAATGAATCAGGAGATAGGCTGGTTTGATG TGCATGATGTTGGGGAGCTCAACACCCGGCTCACAGATGATGTCTCCAAAATTAATGACGGAATTGGTGA CAAAATTGGGATGTTTTTTCAGTCCATAACCACATTTTTAGCCGGTTTTATCATAGGATTTATAAGTGGT TGGAAGCTAACCCTTGTCATTTTGGCTGTCAGCCCTCTTATTGGATTGTCATCTGCTTTGTGGGCAAAGG TATTGACTTCATTTACTAATAAGGAACTCCAGGCTTATGCAAAAGCTGGAGCAGTTGCTGAAGAAGTCTT AGCAGCCATCAGAACTGTGATTGCCTTTGGAGGACAACAGAAGGAACTTGAAAGGTACAATAAAAATTTA GAAGAAGCTAAAAATGTTGGCATAAAGAAAGCTATCACAGCCAGCATTTCGATAGGCATTGCCTACCTGT TGGTCTATGCATCATATGCACTGGCATTCTGGTATGGGACATCCTTGGTCCTCTCAAATGAATATTCTAT TGGAGAAGTGCTTACTGTCTTCTTCTCTATTTTGTTGGGGACTTTTAGTATTGGACACTTGGCCCCAAAC ATAGAAGCCTTTGCAAACGCACGAGGGGCAGCCTTTGAAATCTTCAAGATAATTGATAACGAGCCAAGCA TTGACAGCTTCTCAACAAAGGGCTACAAACCAGACAGTATAATGGGAAACTTAGAGTTTAAAAATGTTCA CTTCAACTACCCATCGAGAAGCGAAGTTCAGATCTTGAAGGGCCTCAATCTGAAGGTGAAGAGCGGACAG ACGGTGGCCCTGGTTGGCAACAGTGGCTGTGGAAAAAGCACAACTGTCCAGCTGATGCAGAGGCTCTACG ACCCCCTGGAGGGCGTGGTCAGTATCGACGGACAAGACATCAGAACCATCAATGTGAGGTATCTGAGGGA GATCATTGGTGTGGTGAGTCAGGAACCTGTGCTGTTTGCCACCACGATCGCCGAGAACATTCGCTATGGC CGAGAAGATGTCACCATGGATGAGATTGAGAAAGCTGTCAAGGAAGCCAATGCCTATGACTTCATCATGA AACTGCCCCACCAATTTGACACCCTGGTTGGTGAGAGAGGGGCGCAGCTGAGTGGGGGACAGAAACAGAG AATCGCCATTGCCCGGGCCCTGGTCCGCAATCCCAAGATCCTTTTGTTGGACGAGGCCACCTCAGCCCTG GATACAGAAAGTGAAGCTGTGGTGCAGGCCGCACTGGATAAGGCTAGAGAAGGCCGGACCACCATTGTGA TAGCTCATCGCTTGTCTACAGTTCGTAATGCTGACGTCATTGCTGGTTTTGATGGTGGTGTCATTGTGGA GCAAGGAAATCATGATGAGCTCATGAGAGAAAAGGGCATTTACTTCAAACTTGTCATGACACAGACTAGA GGAAATGAAATTGAACCAGGAAATAATGCTTATGGATCCCAGAGTGACACTGATGCTTCTGAACTGACTT CAGAAGAATCCAAATCACCTTTAATAAGGAGATCAATTTACAGAAGTGTCCACAGAAAGCAAGACCAAGA GAGAAGACTTAGTATGAAAGAGGCTGTGGATGAAGATGTGCCTCTGGTTTCCTTTTGGCGGATCCTAAAT CTAAATCTAAGTGAATGGCCTTATTTACTTGTTGGCGTACTTTGCGCTGTTATAAATGGGTGCATACAAC CAGTGTTTGCCATAGTATTTTCAAGGATTGTAGGGGTTTTTTCAAGAGATGATGACCATGAAACTAAACG ACAGAATTGTAATTTGTTTTCCCTGTTCTTTCTGGTTATGGGGCTGATTTCTTTTGTTACATATTTCTTT CAGGGCTTCACATTTGGCAAAGCCGGAGAGATCCTCACCAAGCGAGTCCGATACATGGTTTTCAAATCCA TGCTGAGACAGGATATAAGCTGGTTCGATGACCATAAGAACAGCACTGGCTCACTGACCACCAGGCTCGC CAGTGATGCTTCTAGTGTTAAAGGGGCGATGGGCGCCAGGCTTGCTGTAGTTACCCAGAATGTAGCAAAC CTCGGGACAGGAGTCATCCTCTCCTTAGTCTATGGCTGGCAGCTGACACTTCTACTTGTAGTAATTATAC CGCTCATTGTATTGGGCGGAATTATTGAAATGAAGCTGTTGTCTGGCCAAGCCTTGAAGGACAAGAAACA GCTTGAGATCTCTGGGAAGATTGCTACAGAAGCAATTGAAAACTTCCGCACTATTGTCTCTTTGACTCGG GAGCAGAAGTTTGAAACCATGTATGCCCAGAGCTTGCAGGTACCATACAGAAATGCGATGAAGAAAGCAC ACGTGTTTGGGATCACGTTCTCCTTCACCCAGGCCATGATGTATTTTTCTTATGCTGCTTGTTTCCGGTT CGGTGCCTACTTGGTGGCACAACAACTCATGACTTTTGAAAATGTTATGTTGGTATTTTCTGCTGTTGTC TTTGGTGCCATGGCAGCTGGGAATACTAGTTCATTTGCTCCTGACTATGCGAAAGCCAAAGTATCAGCAT CTCATATCATCAGGATCATTGAGAAAACCCCTGAGATTGACAGCTACAGCACAGAGGGCTTGAAGCCTAC TCTGTTAGAAGGAAATGTAAAATTTAATGGAGTCCAGTTTAACTATCCCACCCGACCCAACATCCCAGTG CTTCAGGGGCTGAGCCTCGAGGTGAAGAAGGGCCAGACGTTGGCCCTGGTGGGCAGCAGTGGCTGTGGGA AGAGCACAGTGGTCCAGCTGCTCGAGCGCTTCTACGACCCCATGGCTGGATCAGTGTTTCTAGATGGCAA AGAAATAAAGCAACTGAATGTCCAGTGGCTCCGAGCTCACCTTGGCATTGTGTCCCAGGAGCCCATTCTC TTTGACTGCAGCATTGCAGAGAACATCGCCTATGGAGACAACAGCCGGGCCGTGTCTCATGAGGAGATTG TGAGGGCAGCCAAGGAGGCCAACATCCACCAGTTCATCGACTCACTGCCTGATAAATACAACACCAGAGT AGGAGACAAAGGCACTCAGCTGTCGGGTGGGCAGAAGCAGCGCATCGCCATCGCACGTGCCCTCGTCAGA CAGCCTCACATTTTACTTCTGGACGAAGCAACATCAGCTCTGGATACAGAAAGTGAAAAGGTTGTCCAGG AAGCGCTGGACAAAGCCAGGGAAGGCCGCACCTGCATTGTGATCGCTCACCGCCTGTCCACCATCCAGAA CGCGGACTTGATCGTGGTGATTGAGAACGGCAAAGTCAAGGAGCACGGCACCCACCAGCAGCTGCTGGCG CAGAAGGGCATCTACTTCTCAATGGTCCAGGCTGGAGCAAAGCGCTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227343 representing NM_011075
Red=Cloning site Green=Tags(s) MEFEENLKGRADKNFSKMGKKSKKEKKEKKPAVGVFGMFRYADWLDKLCMILGTLAAIIHGTLLPLLMLV FGNMTDSFTKAEASILPSITNQSGPNSTLIISNSSLEEEMAIYAYYYTGIGAGVLIVAYIQVSLWCLAAG RQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINDGIGDKIGMFFQSITTFLAGFIIGFISG WKLTLVILAVSPLIGLSSALWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQQKELERYNKNL EEAKNVGIKKAITASISIGIAYLLVYASYALAFWYGTSLVLSNEYSIGEVLTVFFSILLGTFSIGHLAPN IEAFANARGAAFEIFKIIDNEPSIDSFSTKGYKPDSIMGNLEFKNVHFNYPSRSEVQILKGLNLKVKSGQ TVALVGNSGCGKSTTVQLMQRLYDPLEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYG REDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSAL DTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQTR GNEIEPGNNAYGSQSDTDASELTSEESKSPLIRRSIYRSVHRKQDQERRLSMKEAVDEDVPLVSFWRILN LNLSEWPYLLVGVLCAVINGCIQPVFAIVFSRIVGVFSRDDDHETKRQNCNLFSLFFLVMGLISFVTYFF QGFTFGKAGEILTKRVRYMVFKSMLRQDISWFDDHKNSTGSLTTRLASDASSVKGAMGARLAVVTQNVAN LGTGVILSLVYGWQLTLLLVVIIPLIVLGGIIEMKLLSGQALKDKKQLEISGKIATEAIENFRTIVSLTR EQKFETMYAQSLQVPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAQQLMTFENVMLVFSAVV FGAMAAGNTSSFAPDYAKAKVSASHIIRIIEKTPEIDSYSTEGLKPTLLEGNVKFNGVQFNYPTRPNIPV LQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAHLGIVSQEPIL FDCSIAENIAYGDNSRAVSHEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVR QPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIENGKVKEHGTHQQLLA QKGIYFSMVQAGAKRS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011075 |
ORF Size | 3828 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011075.2, NP_035205.1 |
RefSeq Size | 4344 bp |
RefSeq ORF | 3831 bp |
Locus ID | 18669 |
UniProt ID | P06795 |
Cytogenetics | 5 3.43 cM |
Gene Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene encodes a membrane glycoprotein which confers a multidrug-resistance phenotype. The protein encoded by the human gene is an ATP-dependent drug efflux pump for xenobiotic compounds which is responsible for decreased drug accumulation in multidrug-resistant cells and mediates the development of resistance to anticancer drugs. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224124 | Abcb1b (untagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1B (Abcb1b), (10ug) |
USD 1,778.00 |
|
MR227343 | Abcb1b (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1B (Abcb1b) |
USD 1,666.00 |
|
MR227343L3 | Lenti ORF clone of Abcb1b (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1B (Abcb1b) |
USD 1,966.00 |
|
MR227343L4 | Lenti ORF clone of Abcb1b (mGFP-tagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1B (Abcb1b) |
USD 1,966.00 |
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