Kcnj10 (NM_001039484) Mouse Tagged ORF Clone

CAT#: MG227234

Kcnj10 (tGFP-tagged) - Mouse potassium inwardly-rectifying channel subfamily J member 10 (Kcnj10), (10ug)

Product Images

Circular map for MG227234

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kcnj10
• Synonyms: BIR10; BIRK-1; Kir1.2; Kir4.1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227234 representing NM_001039484
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTCGGTCGCTAAGGTCTATTACAGTCAGACGACTCAGACAGAGAGCCGCCCCCTAGTGGCCCCAG
  GAATACGCCGGAGGAGGGTCCTCACGAAAGACGGCCGGAGCAATGTGAGAATGGAGCACATTGCTGACAA
  ACGTTTCCTCTACCTCAAGGATCTATGGACGACCTTCATTGACATGCAATGGCGCTACAAGCTTCTGCTC
  TTCTCTGCAACCTTTGCAGGCACGTGGTTCCTCTTTGGTGTGGTGTGGTATCTGGTAGCTGTGGCCCATG
  GGGACCTGTTGGAGCTGGGACCTCCTGCCAACCACACGCCTTGTGTGGTGCAGGTGCACACGCTCACCGG
  AGCCTTCCTCTTCTCCCTGGAATCCCAGACCACCATCGGCTATGGCTTCCGCTACATCAGTGAGGAATGC
  CCACTGGCCATCGTGCTTCTTATTGCGCAGCTGGTGCTCACCACCATTCTGGAAATCTTCATCACAGGTA
  CCTTCCTTGCAAAGATTGCCCGGCCTAAGAAGAGGGCCGAGACGATCCGCTTCAGCCAGCATGCCGTTGT
  GGCTTCCCATAACGGGAAGCCTTGCCTTATGATCCGGGTTGCCAATATGCGGAAGAGTCTCCTCATTGGA
  TGCCAGGTGACAGGCAAACTGCTTCAAACGCACCAGACAAAGGAGGGTGAGAATATTCGGCTCAACCAGG
  TCAACGTGACTTTCCAAGTAGACACAGCCTCAGACAGCCCCTTTCTCATCCTACCCCTGACTTTCTACCA
  CGTGGTAGATGAGACCAGCCCCTTAAAAGATCTCCCGCTCCGCAGTGGGGAGGGGGACTTTGAGCTGGTG
  CTGATCCTGAGTGGGACAGTGGAGTCCACCAGTGCCACCTGCCAAGTTCGCACTTCCTACCTACCGGAGG
  AGATCCTCTGGGGTTACGAGTTCACGCCTGCGATCTCACTGTCAGCCAGTGGCAAATACATAGCTGACTT
  CAGCCTTTTCGACCAGGTTGTGAAAGTGGCATCTCCCAGTGGTCTCCGCGATAGCACCGTACGCTATGGA
  GACCCCGAGAAGCTCAAGTTGGAGGAGTCATTAAGAGAGCAAGCTGAAAAGGAAGGCAGTGCCCTTAGTG
  TGCGCATCAGCAACGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227234 representing NM_001039484
  Red=Cloning site Green=Tags(s)
  
  MTSVAKVYYSQTTQTESRPLVAPGIRRRRVLTKDGRSNVRMEHIADKRFLYLKDLWTTFIDMQWRYKLLL
  FSATFAGTWFLFGVVWYLVAVAHGDLLELGPPANHTPCVVQVHTLTGAFLFSLESQTTIGYGFRYISEEC
  PLAIVLLIAQLVLTTILEIFITGTFLAKIARPKKRAETIRFSQHAVVASHNGKPCLMIRVANMRKSLLIG
  CQVTGKLLQTHQTKEGENIRLNQVNVTFQVDTASDSPFLILPLTFYHVVDETSPLKDLPLRSGEGDFELV
  LILSGTVESTSATCQVRTSYLPEEILWGYEFTPAISLSASGKYIADFSLFDQVVKVASPSGLRDSTVRYG
  DPEKLKLEESLREQAEKEGSALSVRISNV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039484
• ORF Size: 1137 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039484.1, NP_001034573.1
• RefSeq Size: 5407 bp
• RefSeq ORF: 1140 bp
• Locus ID: 16513
• UniProt ID: Q9JM63
• Cytogenetics: 1 79.69 cM
• Gene Summary: May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules (By similarity).[UniProtKB/Swiss-Prot Function]