Nck2 (NM_010879) Mouse Tagged ORF Clone
CAT#: MG227128
- TrueORF®
Nck2 (tGFP-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_010879" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Nck2 |
Synonyms | 4833426I10Rik; Grb4; Nck-2; NCKbeta |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227128 representing NM_010879
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGAAGAAGTCATTGTCATAGCCAAGTGGGACTACACTGCCCAGCAGGACCAGGAGCTCGACATCA GGAAGAACGAGCGGTTGTGGCTGCTAGATGACTCCAAGACGTGGTGGCGGGTGAGGAATGCTGCCAACAG GACAGGCTACGTGCCTTCCAACTATGTGGAGCGTAAGAACAGCCTGAAGAAGGGCTCCCTGGTGAAGAAC CTCAAGGACACACTAGGCCTCGGAAAGACGCGCAGGAAGCCAAGCGCTCGGGATGCTTCCCCAACGCCAA GCACTGATGCGGAGTACCCAGCCAATGGCAGCGGTGCTGACCGCATCTATGACCTCAACATCCCAGCCTT TGTCAAGTTCGCCTACGTAGCAGAACGAGAGGATGAGCTGTCCCTGGTGAAGGGCTCCCGTGTCACAGTT ATGGAGAAATGCAGTGATGGCTGGTGGCGTGGCAGCTTCAACGGGCAGATCGGCTGGTTCCCCTCCAACT ACGTTTTGGAGGAAGCGGACGAAGCTGCTGCTGAGGCCCCCAGCTTCCTGAGCCTACGTCGGGGCACTGC ACTGAGCAATGGGCAGGGTGCACGGGTGCTGCACGTGGTGCAGACGCTGTACCCCTTCAGCTCGGTCACT GAGGAGGAGCTTAGTTTCGAGAAGGGGGAGACCATGGAGGTGATTGAGAAGCCGGAGAATGACCCTGAAT GGTGGAAATGCAAAAATGCCCGAGGCCAAGTGGGCCTGGTCCCCAAAAACTACGTGGTTGTTCTCAGTGA TGGGCCTGCTCTGCACCCCGCTCACACCCCCCAGATCAGCTACACCGGGCCTTCAGCCAGCGGGCGCTTT GCTGGTCGGGAGTGGTACTATGGCAACGTGACACGGCACCAGGCCGAGTGTGCGCTCAATGAGCGGGGCG TCGAGGGCGACTTCCTCATTAGGGACAGCGAGTCCTCGCCCAGTGACTTCTCCGTGTCTCTCAAAGCGTC AGGGAGAAACAAGCACTTCAAGGTGCAGCTGGTGGACAGCGTCTACTGCATTGGGCAGCGGCGGTTCCAC AGCATGGACGAGCTTGTGGAGCACTACAAGAAGGCCCCCATCTTCACCAGCGAGCACGGGGAGAAGCTCT ACCTTGTCCGAGCCCTACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227128 representing NM_010879
Red=Cloning site Green=Tags(s) MTEEVIVIAKWDYTAQQDQELDIRKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKN LKDTLGLGKTRRKPSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTV MEKCSDGWWRGSFNGQIGWFPSNYVLEEADEAAAEAPSFLSLRRGTALSNGQGARVLHVVQTLYPFSSVT EEELSFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHTPQISYTGPSASGRF AGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGRNKHFKVQLVDSVYCIGQRRFH SMDELVEHYKKAPIFTSEHGEKLYLVRALQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010879 |
ORF Size | 1140 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010879.3, NP_035009.3 |
RefSeq Size | 2789 bp |
RefSeq ORF | 1143 bp |
Locus ID | 17974 |
UniProt ID | O55033 |
Cytogenetics | 1 C1.1 |
Gene Summary | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209015 | Nck2 (untagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2), (10ug) |
USD 503.00 |
|
MR227128 | Nck2 (Myc-DDK-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2) |
USD 457.00 |
|
MR227128L3 | Lenti ORF clone of Nck2 (Myc-DDK-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2) |
USD 757.00 |
|
MR227128L4 | Lenti ORF clone of Nck2 (mGFP-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2) |
USD 757.00 |
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