Gck (NM_010292) Mouse Tagged ORF Clone
CAT#: MG227074
- TrueORF®
Gck (tGFP-tagged) - Mouse glucokinase (Gck), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_010292" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Gck |
Synonyms | Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP; MODY2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227074 representing NM_010292
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGATGACAGAGCCAGGATGGAGGCCACCAAGAAGGAAAAGGTAGAGCAGATCCTGGCAGAGTTCC AGCTGCAGGAGGAAGACCTGAAGAAGGTGATGAGCCGGATGCAGAAGGAGATGGACCGTGGCCTGAAGCT GGAGACCCATCAGGAGGCCAGTGTAAAGATGTTGCCCACCTACGTGCGTTCCACCCCAGAAGGCTCAGAA GTTGGAGACTTTCTCTCCTTAGACCTGGGAGGAACCAACTTCAGGGTGATGCTGGTGAAAGTGGGGGAGG GGGAGGCAGGACAGTGGAGCGTGAAGACGAAACACCAGATGTATTCCATCCCCGAGGACGCCATGACGGG CACTGCGGAGATGCTCTTTGACTACATCTCTGAGTGCATCTCTGACTTCCTGGACAAGCATCAGATGAAA CACAAGAAACTACCCCTGGGCTTCACCTTCTCCTTCCCTGTAAGGCACGAAGACATAGACAAGGGCATCC TGCTCAACTGGACCAAGGGCTTCAAGGCCTCCGGAGCAGAAGGGAACAACATCGTGGGACTTCTCCGAGA TGCTATCAAGAGGAGAGGGGACTTTGAGATGGATGTGGTGGCAATGGTGAATGACACGGTGGCCACAATG ATCTCCTGCTACTATGAAGACCGCCAATGTGAGGTCGGCATGATTGTGGGCACCGGCTGCAACGCCTGCT ACATGGAGGAGATGCAGAATGTGGAGCTGGTGGAAGGCGATGAGGGGCGCATGTGTGTCAACACAGAGTG GGGCGCCTTCGGGAACTCCGGTGAGCTGGACGAGTTCCTCCTGGAGTACGACCGGATGGTGGATGAGAGC TCAGTGAACCCCGGTCAGCAGCTGTACGAAAAGATCATTGGCGGAAAGTACATGGGCGAGCTGGTACGAC TTGTGCTGCTCAAGCTGGTAGAGGAGAATCTTCTGTTCCACGGAGAGGCCTCAGAGCAGCTGCGCACACG TGGTGCTTTTGAGACCCGTTTTGTGTCGCAGGTGGAGAGCGACTCTGGGGACCGAAGGCAGATCCTTAAC ATCCTGAGCACTCTGGGCCTTCGACCCTCTGTCGCCGACTGCGACATTGTGCGCCGTGCCTGTGAAAGCG TGTCCACTCGCGCCGCCCACATGTGCTCAGCAGGACTAGCGGGGGTCATAAATCGCATGCGCGAAAGCCG CAGTGAGGACGTGATGCGCATCACGGTGGGCGTGGATGGCTCCGTGTACAAGCTGCACCCGAGCTTCAAG GAGCGGTTTCACGCCAGTGTGCGCAGGCTGACACCCAACTGCGAAATCACCTTCATTGAATCAGAGGAGG GCAGCGGCAGGGGAGCCGCACTGGTCTCTGCGGTGGCCTGCAAGAAGGCTTGCATGCTGGGCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227074 representing NM_010292
Red=Cloning site Green=Tags(s) MLDDRARMEATKKEKVEQILAEFQLQEEDLKKVMSRMQKEMDRGLKLETHQEASVKMLPTYVRSTPEGSE VGDFLSLDLGGTNFRVMLVKVGEGEAGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMK HKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNIVGLLRDAIKRRGDFEMDVVAMVNDTVATM ISCYYEDRQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGNSGELDEFLLEYDRMVDES SVNPGQQLYEKIIGGKYMGELVRLVLLKLVEENLLFHGEASEQLRTRGAFETRFVSQVESDSGDRRQILN ILSTLGLRPSVADCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFK ERFHASVRRLTPNCEITFIESEEGSGRGAALVSAVACKKACMLGQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010292 |
ORF Size | 1395 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010292.5, NP_034422.2 |
RefSeq Size | 2245 bp |
RefSeq ORF | 1398 bp |
Locus ID | 103988 |
UniProt ID | P52792 |
Cytogenetics | 11 3.88 cM |
Gene Summary | Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216354 | Gck (untagged) - Mouse glucokinase (Gck), (10ug) |
USD 503.00 |
|
MR227074 | Gck (Myc-DDK-tagged) - Mouse glucokinase (Gck) |
USD 457.00 |
|
MR227074L3 | Lenti ORF clone of Gck (Myc-DDK-tagged) - Mouse glucokinase (Gck) |
USD 757.00 |
|
MR227074L4 | Lenti ORF clone of Gck (mGFP-tagged) - Mouse glucokinase (Gck) |
USD 757.00 |
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