Tlr9 (NM_031178) Mouse Tagged ORF Clone
CAT#: MG227011
- TrueORF®
Tlr9 (tGFP-tagged) - Mouse toll-like receptor 9 (Tlr9), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_031178" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Tlr9 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227011 representing NM_031178
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCTCCGTCGAAGGACTCTGCACCCCTTGTCCCTCCTGGTACAGGCTGCAGTGCTGGCTGAGACTC TGGCCCTGGGTACCCTGCCTGCCTTCCTACCCTGTGAGCTGAAGCCTCATGGCCTGGTGGACTGCAATTG GCTGTTCCTGAAGTCTGTACCCCGTTTCTCTGCGGCAGCATCCTGCTCCAACATCACCCGCCTCTCCTTG ATCTCCAACCGTATCCACCACCTGCACAACTCTGACTTCGTCCACCTGTCCAACCTGCGGCAGCTGAACC TCAAGTGGAACTGTCCACCCACTGGCCTTAGCCCCCTGCACTTCTCTTGCCACATGACCATTGAGCCCAG AACCTTCCTGGCTATGCGTACACTGGAGGAGCTGAACTTGAGCTATAATGGTATCACCACTGTGCCCCGA CTGCCCAGCTCCCTGGTGAATCTGAGCCTGAGCCACACCAACATCCTGGTTCTAGATGCTAACAGCCTCG CCGGCCTATACAGCCTGCGCGTTCTCTTCATGGACGGGAACTGCTACTACAAGAACCCCTGCACAGGAGC GGTGAAGGTGACCCCAGGCGCCCTCCTGGGCCTGAGCAATCTCACCCATCTGTCTCTGAAGTATAACAAC CTCACAAAGGTGCCCCGCCAACTGCCCCCCAGCCTGGAGTACCTCCTGGTGTCCTATAACCTCATTGTCA AGCTGGGGCCTGAAGACCTGGCCAATCTGACCTCCCTTCGAGTACTTGATGTGGGTGGGAATTGCCGTCG CTGTGACCATGCCCCCAATCCCTGTATAGAATGTGGCCAAAAGTCCCTCCACCTGCACCCTGAGACCTTC CATCACCTGAGCCATCTGGAAGGCCTGGTGCTGAAGGACAGCTCTCTCCATACACTGAACTCTTCCTGGT TCCAAGGTCTGGTCAACCTCTCGGTGCTGGACCTAAGCGAGAACTTTCTCTATGAAAGCATCACCCACAC CAATGCCTTTCAGAACCTAACCCGCCTGCGCAAGCTCAACCTGTCCTTCAATTACCGCAAGAAGGTATCC TTTGCCCGCCTCCACCTGGCAAGTTCCTTTAAGAACCTGGTGTCACTGCAGGAGCTGAACATGAACGGCA TCTTCTTCCGCTTGCTCAACAAGTACACGCTCAGATGGCTGGCCGATCTGCCCAAACTCCACACTCTGCA TCTTCAAATGAACTTCATCAACCAGGCACAGCTCAGCATCTTTGGTACCTTCCGAGCCCTTCGCTTTGTG GACTTGTCAGACAATCGCATCAGTGGGCCTTCAACGCTGTCAGAAGCCACCCCTGAAGAGGCAGATGATG CAGAGCAGGAGGAGCTGTTGTCTGCGGATCCTCACCCAGCTCCGCTGAGCACCCCTGCTTCTAAGAACTT CATGGACAGGTGTAAGAACTTCAAGTTCACCATGGACCTGTCTCGGAACAACCTGGTGACTATCAAGCCA GAGATGTTTGTCAATCTCTCACGCCTCCAGTGTCTTAGCCTGAGCCACAACTCCATTGCACAGGCTGTCA ATGGCTCTCAGTTCCTGCCGCTGACTAATCTGCAGGTGCTGGACCTGTCCCATAACAAACTGGACTTGTA CCACTGGAAATCGTTCAGTGAGCTACCACAGTTGCAGGCCCTGGACCTGAGCTACAACAGCCAGCCCTTT AGCATGAAGGGTATAGGCCACAATTTCAGTTTTGTGACCCATCTGTCCATGCTACAGAGCCTTAGCCTGG CACACAATGACATTCATACCCGTGTGTCCTCACATCTCAACAGCAACTCAGTGAGGTTTCTTGACTTCAG CGGCAACGGTATGGGCCGCATGTGGGATGAGGGGGGCCTTTATCTCCATTTCTTCCAAGGCCTGAGTGGC CTGCTGAAGCTGGACCTGTCTCAAAATAACCTGCATATCCTCCGGCCCCAGAACCTTGACAACCTCCCCA AGAGCCTGAAGCTGCTGAGCCTCCGAGACAACTACCTATCTTTCTTTAACTGGACCAGTCTGTCCTTCCT ACCCAACCTGGAAGTCCTAGACCTGGCAGGCAACCAGCTAAAGGCCCTGACCAATGGCACCCTGCCTAAT GGCACCCTCCTCCAGAAACTCGATGTCAGTAGCAACAGTATCGTCTCTGTGGTCCCAGCCTTCTTCGCTC TGGCGGTCGAGCTGAAAGAGGTCAACCTCAGCCACAACATTCTCAAGACGGTGGATCGCTCCTGGTTTGG GCCCATTGTGATGAACCTGACAGTTCTAGACGTGAGAAGCAACCCTCTGCACTGTGCCTGTGGGGCAGCC TTCGTAGACTTACTGTTGGAGGTGCAGACCAAGGTGCCTGGCCTGGCTAATGGTGTGAAGTGTGGCAGCC CCGGCCAGCTGCAGGGCCGTAGCATCTTCGCGCAGGACCTGCGGCTGTGCCTGGATGAGGTCCTCTCTTG GGACTGCTTTGGCCTTTCACTCTTGGCTGTGGCCGTGGGCATGGTGGTGCCTATACTGCACCATCTCTGC GGCTGGGACGTCTGGTACTGTTTTCATCTGTGCCTGGCATGGCTACCTTTGCTAGCCCGCAGCCGACGCA GCGCCCAAACTCTCCCTTATGATGCCTTCGTGGTGTTCGATAAGGCACAGAGCGCAGTTGCCGACTGGGT GTATAACGAGCTGCGGGTGCGGCTGGAGGAGCGGCGCGGCCGCCGAGCCCTACGCTTGTGTCTGGAGGAC CGAGATTGGCTGCCTGGCCAGACGCTCTTCGAGAACCTCTGGGCTTCCATCTATGGGAGCCGCAAGACTC TATTTGTGCTGGCCCACACGGACCGCGTCAGTGGCCTCCTGCGCACCAGCTTCCTGCTGGCTCAGCAGCG CCTGTTGGAAGACCGCAAGGACGTGGTGGTGTTGGTGATCCTGCGTCCGGATGCCCACCGCTCCCGCTAT GTGCGACTGCGCCAGCGTCTCTGCCGCCAGAGTGTGCTCTTCTGGCCCCAGCAGCCCAACGGGCAGGGGG GCTTCTGGGCCCAGCTGAGTACAGCCCTGACTAGGGACAACCGCCACTTCTATAACCAGAACTTCTGCCG GGGACCTACAGCAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227011 representing NM_031178
Red=Cloning site Green=Tags(s) MVLRRRTLHPLSLLVQAAVLAETLALGTLPAFLPCELKPHGLVDCNWLFLKSVPRFSAAASCSNITRLSL ISNRIHHLHNSDFVHLSNLRQLNLKWNCPPTGLSPLHFSCHMTIEPRTFLAMRTLEELNLSYNGITTVPR LPSSLVNLSLSHTNILVLDANSLAGLYSLRVLFMDGNCYYKNPCTGAVKVTPGALLGLSNLTHLSLKYNN LTKVPRQLPPSLEYLLVSYNLIVKLGPEDLANLTSLRVLDVGGNCRRCDHAPNPCIECGQKSLHLHPETF HHLSHLEGLVLKDSSLHTLNSSWFQGLVNLSVLDLSENFLYESITHTNAFQNLTRLRKLNLSFNYRKKVS FARLHLASSFKNLVSLQELNMNGIFFRLLNKYTLRWLADLPKLHTLHLQMNFINQAQLSIFGTFRALRFV DLSDNRISGPSTLSEATPEEADDAEQEELLSADPHPAPLSTPASKNFMDRCKNFKFTMDLSRNNLVTIKP EMFVNLSRLQCLSLSHNSIAQAVNGSQFLPLTNLQVLDLSHNKLDLYHWKSFSELPQLQALDLSYNSQPF SMKGIGHNFSFVTHLSMLQSLSLAHNDIHTRVSSHLNSNSVRFLDFSGNGMGRMWDEGGLYLHFFQGLSG LLKLDLSQNNLHILRPQNLDNLPKSLKLLSLRDNYLSFFNWTSLSFLPNLEVLDLAGNQLKALTNGTLPN GTLLQKLDVSSNSIVSVVPAFFALAVELKEVNLSHNILKTVDRSWFGPIVMNLTVLDVRSNPLHCACGAA FVDLLLEVQTKVPGLANGVKCGSPGQLQGRSIFAQDLRLCLDEVLSWDCFGLSLLAVAVGMVVPILHHLC GWDVWYCFHLCLAWLPLLARSRRSAQTLPYDAFVVFDKAQSAVADWVYNELRVRLEERRGRRALRLCLED RDWLPGQTLFENLWASIYGSRKTLFVLAHTDRVSGLLRTSFLLAQQRLLEDRKDVVVLVILRPDAHRSRY VRLRQRLCRQSVLFWPQQPNGQGGFWAQLSTALTRDNRHFYNQNFCRGPTAE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_031178 |
ORF Size | 3096 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031178.2, NP_112455.2 |
RefSeq Size | 3471 bp |
RefSeq ORF | 3099 bp |
Locus ID | 81897 |
UniProt ID | Q9EQU3 |
Cytogenetics | 9 F1 |
Gene Summary | Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:18931679, PubMed:21402738, PubMed:14993594, PubMed:17474149, PubMed:25686612, PubMed:18820679). Plays a role in defense against systemic mouse cytomegalovirus infection (PubMed:14993594). Controls lymphocyte response to Helicobacter infection (PubMed:17474149). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223287 | Tlr9 (untagged) - Mouse toll-like receptor 9 (Tlr9), (10ug) |
USD 1,349.00 |
|
MR227011 | Tlr9 (Myc-DDK-tagged) - Mouse toll-like receptor 9 (Tlr9) |
USD 1,347.00 |
|
MR227011L3 | Lenti ORF clone of Tlr9 (Myc-DDK-tagged) - Mouse toll-like receptor 9 (Tlr9) |
USD 1,647.00 |
|
MR227011L4 | Lenti ORF clone of Tlr9 (mGFP-tagged) - Mouse toll-like receptor 9 (Tlr9) |
USD 1,647.00 |
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