Myh2 (NM_001039545) Mouse Tagged ORF Clone

CAT#: MG226659

Myh2 (tGFP-tagged) - Mouse myosin heavy polypeptide 2 skeletal muscle adult (Myh2), (10ug)

Product Images

Circular map for MG226659

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Myh2
• Synonyms: MHC2A; Myh2a; MyHC-IIa; Myhs-f; Myhs-f1; Myhsf1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226659 representing NM_001039545
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCGACGCCGAGATGGCCGTTTTCGGGGAGGCTGCCCCTTACCTCCGGAAGTCCGAAAAGGAGC
  GAATCGAGGCCCAGAATAGGCCTTTTGATGCCAAAACATCTGTCTTTGTGGCGGAGCCCAAGGAATCCTT
  TGTCAAAGGAACCATTCAGAGCAAAGATGCAGGGAAAGTGACTGTGAAAACAGAAGCAGGAGCGACCCTG
  ACCGTGAAAGAAGACCAGATCTTCCCCATGAACCCTCCCAAGTACGACAAGATCGAGGACATGGCCATGA
  TGACCCACCTGCACGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGATCTACAC
  CTACTCAGGCCTCTTCTGTGTCACCGTCAACCCTTACAAATGGCTGCCGGTGTACAACCCCGAGGTGGTG
  GCGGCCTATCGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGATAACGCCTACC
  AGTTCATGCTAACAGACAGGGAGAATCAGTCAATCCTGATCACCGGAGAATCCGGGGCCGGGAAGACTGT
  GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGACAAGAAGAAGGAGGAGGCA
  ACTTCTGGCAAAATGCAGGGGACGCTGGAGGACCAAATCATCAGTGCCAACCCCCTGCTGGAGGCCTTTG
  GGAACGCCAAGACCGTGAGGAATGACAACTCGTCTCGCTTTGGTAAATTCATCAGGATCCACTTTGGCAC
  TACGGGGAAACTGGCATCTGCTGACATCGAGACCTATCTGCTAGAGAAGTCCCGGGTCACTTTCCAGCTT
  AAGGCGGAAAGAAGCTACCATATATTTTATCAGATCACATCCAACAAGAAGCCAGAGCTGATCGAAATGC
  TGCTGATCACCACGAACCCATATGATTACCCATTTGTCAGCCAGGGGGAGATCAGTGTGGCCAGCATTGA
  TGACCAGGAAGAGCTGATGGCCACTGATAGTGCTATCGACATTTTGGGCTTTACAAATGATGAAAAAGTC
  TCCATCTATAAGCTCACCGGTGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAGG
  AGCAGGCAGAGCCAGATGGCACCGAAGTTGCTGACAAAGCTGCCTATCTTCAGGGTCTGAACTCTGCTGA
  CCTGCTCAAAGCCCTCTGCTACCCCAGGGTCAAGGTCGGCAATGAGTACGTCACCAAAGGCCAGACTGTC
  GAGCAGGTGACCAATGCTGTGGGTGCCCTGGCCAAGGCCATGTATGAGAAGATGTTCCTGTGGATGGTCA
  CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG
  CTTTGAGATCTTTGATTTCAACAGCCTGGAACAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG
  TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAATACAAGAAGGAGGGCATCGAGTGGACCTTCA
  TCGACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCT
  GGAAGAGGAGTGCATGTTCCCTAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAGCAGCATCTT
  GGAAAGTCTGCCAACTTCCAGAAGCCTAAGGTGGTCAAAGGCAAGGCCGAAGCCCACTTCTCCCTCATCC
  ACTATGCGGGCACCGTGGATTACAACATCACTGGCTGGCTGGACAAGAACAAGGACCCACTGAACGAGAC
  CGTGGTGGGGCTGTACCAGAAGTCTTCAGTGAAAACTCTGGCTTATCTCTTCTCTGGGGCACAAACTGCT
  GAAGCAGAGGCAAGTAGTGGTGGAGCTGCCAAGAAAGGTGCCAAGAAGAAGGGGTCCTCTTTTCAGACCG
  TGTCTGCCCTGTTCAGAGAGAATTTGAACAAGCTGATGACCAATCTCAGGAGCACCCATCCTCATTTCGT
  GAGGTGTATCATCCCCAATGAAACCAAGACTCCCGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTG
  AGGTGTAACGGGGTGCTGGAGGGCATCCGCATCTGCAGAAAGGGCTTCCCCAGCAGGATCCTCTATGCAG
  ACTTCAAGCAGAGATACAAAGTGTTAAACGCAAGTGCCATTCCTGAGGGTCAGTACATCGATAGCAAGAA
  GGCTTCTGAGAAGCTCCTGGGCTCCATTGACATTGACCACACTCAGTATAAGTTTGGTCACACCAAGGTC
  TTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGAGAGATGACAAGTTGGCCCAGCTTATTACCC
  GGACCCAGGCTATGTGCAGAGGGTTCTTGGCGAGAGTGGAGTACCAGAAGATGGTGGAGAGAAGGGAGTC
  CATCTTCTGCATCCAGTACAACATCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTC
  TTCAAGATCAAACCCCTGCTGAAGAGCGCAGAGACCGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCC
  AGAAAACCAAAGATGACCTTGCCAAGTCAGAGGCAAAGAGGAAGGAACTTGAAGAAAAGATGGTGTCCCT
  CTTGAAAGAAAAAAATGACTTGCAACTTCAAGTTCAGGCTGAAGCTGAAGGCTTGGCCGATGCAGAGGAG
  CGATGCGACCAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACCGAGAGGGCCG
  AGGATGAGGAGGAGATCAACGCAGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCT
  GAAGAAGGACATCGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAAC
  AAGGTGAAAAACCTGACAGAGGAGATGGCAGGCCTGGACGAAACCATCGCCAAGCTGACCAAGGAGAAGA
  AGGCCCTCCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCT
  GACCAAAGCCAAAATCAAGCTGGAACAGCAAGTGGATGACCTTGAAGGGTCCTTGGAGCAAGAAAAGAAA
  CTTCGCATGGACCTAGAAAGAGCCAAGAGAAAACTTGAGGGCGACTTGAAGTTAGCCCAGGAGTCCATCA
  TGGACATTGAAAATGAGAAACAGCAGCTCGATGAAAGGCTCAAAAAGAAAGAGTTTGAAATGAGCAATCT
  GCAGAGCAAGATTGAGGACGAGCAGGCCATCGGCATTCAGCTGCAGAAGAAGATCAAGGAGTTGCAGGCC
  CGCATCGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCT
  CTGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGCGGAGCCACTTCAGCCCA
  GATCGAGATGAACAAGAAGAGAGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACGCTG
  CAGCATGAAGCCACAGCAGCCACCCTGAGGAAGAAGCATGCGGACAGCGTGGCCGAGCTCGGGGAGCAGA
  TCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGATGAAGATGGAGATCGATGA
  TCTTGCCAGTAATGTAGAGACAGTGTCTAAGGCCAAGGGAAACCTCGAGAAGATGTGCCGCACCCTGGAG
  GACCAGGTGAGTGAGCTGAAGTCAAAGGAGGAGGAACAGCAGCGACTGATCAACGACCTGACAACCCAGA
  GAGGACGACTGCAGACCGAATCCGGTGAATTTTCCAGGCAGCTTGATGAGAAGGAAGCGCTGGTATCTCA
  GTTATCAAGGGGGAAGCAAGCATTCACTCAACAGATTGAGGAGCTAAAGAGGCAGCTTGAAGAGGAAGTA
  AAGGCCAAGAACGCGCTGGCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGT
  ACGAGGAGGAGCAGGAGTCTAAGGCTGAACTGCAGAGGGCGCTGTCCAAGGCCAACAGCGAGGTGGCCCA
  GTGGAGGACCAAATATGAGACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAAGCCAAGAAGAAGCTG
  GCTCAGCGTCTGCAGGCGGCTGAGGAGCACGTAGAAGCCGTGAACGCCAAGTGCGCTTCTCTGGAGAAGA
  CGAAGCAGCGGCTGCAGAACGAGGTGGAGGACCTCATGCTGGATGTGGAGAGGACCAACGCTGCTTGTGC
  CGCCCTGGACAAGAAGCAGAGAAACTTCGACAAGATCCTGGCAGAGTGGAAGCAGAAGTATGAGGAAACC
  CACGCTGAGCTCGAGGCATCCCAGAAGGAGGCCCGCTCCCTGGGCACTGAGCTCTTCAAGATGAAGAATG
  CCTACGAGGAGTCTCTGGATCAGCTAGAAACCCTGAAGCGAGAGAATAAGAACTTACAGCAGGAGATTTC
  TGACCTCACGGAACAGATTGCAGAAGGAGGAAAGCGCATCCACGAACTGGAGAAAATTAAGAAACAAGTC
  GAACAAGAGAAGTGTGAACTTCAGGCTGCTCTAGAAGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAA
  AGATCCTGCGCATCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGA
  TGAGGAGATCGACCAGCTGAAGAGAAACCACATTAGAGTCGTGGAGTCCATGCAGAGCACGCTGGATGCC
  GAGATCAGGAGCAGGAACGACGCCATCAGAATCAAGAAGAAGATGGAGGGAGACCTGAACGAGATGGAAA
  TCCAGCTGAACCACTCCAACCGCATGGCTGCCGAGGCCCTGAGAAACTACAGGAACACACAAGGCATCCT
  CAAGGACACCCAGTTGCACCTGGACGATGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATG
  GTTGAGCGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACGCTGGAGCAGACGGAGA
  GGAGCAGGAAGATTGCAGAGCAGGAGCTGCTGGACGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAA
  CACCAGCCTCATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAAATCCAGGGAGAGATGGAGGAC
  ATCGTCCAGGAAGCCCGCAATGCAGAAGAGAAAGCTAAGAAAGCCATCACTGATGCCGCCATGATGGCGG
  AGGAGCTGAAGAAGGAGCAGGACACTAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGT
  GAAGGACCTGCAGCTGCGTCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAG
  AAACTGGAGGCCAGGGTGCGTGAACTGGAGGGTGAGGTAGAGAGTGAGCAGAAGCGGAATGCTGAGGCCG
  TCAAAGGTCTGCGCAAACACGAGAGACGAGTGAAGGAGCTTACTTACCAGACAGAAGAAGACCGAAAAAA
  TATTCTCAGGCTTCAGGATTTGGTGGATAAACTCCAGGCAAAAGTGAAATCTTACAAGAGACAAGCTGAG
  GAGGCTGAGGAACAATCCAACACAAATCTATCCAAGTTCCGCAAGATCCAGCACGAGCTGGAGGAAGCCG
  AGGAGCGGGCTGACATCGCGGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGGGAGGTTCACACCAA
  AATCATAAGCGAAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226659 representing NM_001039545
  Red=Cloning site Green=Tags(s)
  
  MSSDAEMAVFGEAAPYLRKSEKERIEAQNRPFDAKTSVFVAEPKESFVKGTIQSKDAGKVTVKTEAGATL
  TVKEDQIFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVV
  AAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGDKKKEEA
  TSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQL
  KAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELMATDSAIDILGFTNDEKV
  SIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQGLNSADLLKALCYPRVKVGNEYVTKGQTV
  EQVTNAVGALAKAMYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ
  FFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHL
  GKSANFQKPKVVKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNETVVGLYQKSSVKTLAYLFSGAQTA
  EAEASSGGAAKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQL
  RCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQYIDSKKASEKLLGSIDIDHTQYKFGHTKV
  FFKAGLLGLLEEMRDDKLAQLITRTQAMCRGFLARVEYQKMVERRESIFCIQYNIRAFMNVKHWPWMKLF
  FKIKPLLKSAETEKEMATMKEEFQKTKDDLAKSEAKRKELEEKMVSLLKEKNDLQLQVQAEAEGLADAEE
  RCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATEN
  KVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKK
  LRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDERLKKKEFEMSNLQSKIEDEQAIGIQLQKKIKELQA
  RIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATL
  QHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLE
  DQVSELKSKEEEQQRLINDLTTQRGRLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIEELKRQLEEEV
  KAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKL
  AQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKYEET
  HAELEASQKEARSLGTELFKMKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQV
  EQEKCELQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQSTLDA
  EIRSRNDAIRIKKKMEGDLNEMEIQLNHSNRMAAEALRNYRNTQGILKDTQLHLDDALRGQEDLKEQLAM
  VERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMED
  IVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQ
  KLEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAE
  EAEEQSNTNLSKFRKIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039545
• ORF Size: 5826 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039545.2, NP_001034634.2
• RefSeq Size: 6083 bp
• RefSeq ORF: 5829 bp
• Locus ID: 17882
• Cytogenetics: 11 40.59 cM