Usp27x (NM_019461) Mouse Tagged ORF Clone

CAT#: MG226453

Usp27x (tGFP-tagged) - Mouse ubiquitin specific peptidase 27 X chromosome (Usp27x), (10ug)

Product Images

Circular map for MG226453

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Usp27x
• Synonyms: Sfc11; Usp27
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226453 representing NM_019461
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTAAAGACTATGTGTATGACATAGACATTGAGCAAATTGCCAAAGAAGAGCAAGGAGAAGCCTTGA
  AATTACAAGCGTCCACCTCGACAGAGGTTTCTCAGCAACAGTGTTCAGTGCCGGGACTTGGGGAGAAATA
  TCCCACCTGGGAAACGACCAAACCTGAACTAGAACTTCTGGGGCACAACCCACGGAGAAGAAGGATCGCC
  TCCAGCTTTACCATCGGCTTACGAGGACTAATTAATCTTGGCAACACGTGTTTTATGAACTGCATTGTCC
  AGGCCTTGACCCACACTCCAATACTGAGAGATTTCTTTCTCTCTGACAGGCACCGGTGTGAAATGCCCAG
  CCCTGAGTTGTGTCTGGTCTGTGAGATGTCGTCGCTCTTTCGGGAGCTGTATTCTGGAAACCCATCTCCT
  CATGTGCCCTATAAGTTACTGCACCTGGTGTGGATACACGCTCGTCATCTAGCAGGGTACAGGCAGCAGG
  ATGCCCACGAGTTCCTCATCGCTGCGCTAGATGTCCTGCATAGGCACTGCAAAGGTGACGATGTTGGCAA
  GGTGGCCAGCAACCCCAACCACTGTAACTGCATCATAGACCAAATCTTCACAGGTGGCCTGCAGTCCGAT
  GTCACCTGTCAAGCCTGCCATGGTGTCTCCACCACTATAGACCCATGCTGGGACATTAGTCTGGACTTGC
  CTGGCTCTTGCACATCCTTCTGGCCCATGAGCCCTGGGAGGGAGAGCAGTTTGAATGGCGAAAGCCACAT
  CCCAGGCATCACCACTCTCACGGACTGCTTGCGAAGGTTTACAAGGCCAGAGCACTTAGGAAGTAGTGCC
  AAAATCAAGTGTGGTAGTTGCCAAAGCTACCAAGAATCTACCAAACAGCTCACTATGAAGAAGTTACCAG
  TAGTTGCCTGCTTTCATTTCAAACGGTTTGAACACTCAGCGAAACAGAGGCGCAAGATCACTACGTACAT
  TTCCTTTCCTCTGGAGCTGGATATGACACCATTTATGGCGTCAAGTAAAGAGACCCGGGTGAATGGACAG
  TTGCAGCTCCCAACCAATAGTGCGAACAACGAGAATAAGTATTCCTTGTTTGCTGTGGTTAATCACCAAG
  GAACCTTGGAGAGTGGCCACTACACCAGCTTCATTCGGCACCACAGGGACCAGTGGTTCAAGTGTGATGA
  TGCCGTAATCACCAAGGCCAGTATTAAGGATGTGCTGGACAGTGAAGGGTATTTACTGTTCTATCACAAA
  CAGGTCCTGGAACCTGAGCCAGAAAAGGTGAAAGAAATGACTCCACAAGCCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226453 representing NM_019461
  Red=Cloning site Green=Tags(s)
  
  MCKDYVYDIDIEQIAKEEQGEALKLQASTSTEVSQQQCSVPGLGEKYPTWETTKPELELLGHNPRRRRIA
  SSFTIGLRGLINLGNTCFMNCIVQALTHTPILRDFFLSDRHRCEMPSPELCLVCEMSSLFRELYSGNPSP
  HVPYKLLHLVWIHARHLAGYRQQDAHEFLIAALDVLHRHCKGDDVGKVASNPNHCNCIIDQIFTGGLQSD
  VTCQACHGVSTTIDPCWDISLDLPGSCTSFWPMSPGRESSLNGESHIPGITTLTDCLRRFTRPEHLGSSA
  KIKCGSCQSYQESTKQLTMKKLPVVACFHFKRFEHSAKQRRKITTYISFPLELDMTPFMASSKETRVNGQ
  LQLPTNSANNENKYSLFAVVNHQGTLESGHYTSFIRHHRDQWFKCDDAVITKASIKDVLDSEGYLLFYHK
  QVLEPEPEKVKEMTPQAY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019461
• ORF Size: 1314 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019461.4, NP_062334.2
• RefSeq Size: 3240 bp
• RefSeq ORF: 1317 bp
• Locus ID: 54651
• UniProt ID: Q8CEG8
• Cytogenetics: X 3.31 cM
• Gene Summary: Deubiquitinase that can reduce the levels of BCL2L11/BIM ubiquitination and stabilize BCL2L11 in response to the RAF-MAPK-degradation signal. By acting on BCL2L11 levels, may counteract the anti-apoptotic effects of MAPK activity.[UniProtKB/Swiss-Prot Function]