Usp27x (NM_019461) Mouse Untagged Clone

CAT#: MC215820

Usp27x (untagged) - Mouse ubiquitin specific peptidase 27, X chromosome (Usp27x), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp27x
• Synonyms: Sfc11; Usp27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215820 representing NM_019461
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTAAAGACTATGTGTATGACATAGACATTGAGCAAATTGCCAAAGAAGAGCAAGGAGAAGCCTTGA
  AATTACAAGCGTCCACCTCGACAGAGGTTTCTCAGCAACAGTGTTCAGTGCCGGGACTTGGGGAGAAATA
  TCCCACCTGGGAAACGACCAAACCTGAACTAGAACTTCTGGGGCACAACCCACGGAGAAGAAGGATCGCC
  TCCAGCTTTACCATCGGCTTACGAGGACTAATTAATCTTGGCAACACGTGTTTTATGAACTGCATTGTCC
  AGGCCTTGACCCACACTCCAATACTGAGAGATTTCTTTCTCTCTGACAGGCACCGGTGTGAAATGCCCAG
  CCCTGAGTTGTGTCTGGTCTGTGAGATGTCGTCGCTCTTTCGGGAGCTGTATTCTGGAAACCCATCTCCT
  CATGTGCCCTATAAGTTACTGCACCTGGTGTGGATACACGCTCGTCATCTAGCAGGGTACAGGCAGCAGG
  ATGCCCACGAGTTCCTCATCGCTGCGCTAGATGTCCTGCATAGGCACTGCAAAGGTGACGATGTTGGCAA
  GGTGGCCAGCAACCCCAACCACTGTAACTGCATCATAGACCAAATCTTCACAGGTGGCCTGCAGTCCGAT
  GTCACCTGTCAAGCCTGCCATGGTGTCTCCACCACTATAGACCCATGCTGGGACATTAGTCTGGACTTGC
  CTGGCTCTTGCACATCCTTCTGGCCCATGAGCCCTGGGAGGGAGAGCAGTTTGAATGGCGAAAGCCACAT
  CCCAGGCATCACCACTCTCACGGACTGCTTGCGAAGGTTTACAAGGCCAGAGCACTTAGGAAGTAGTGCC
  AAAATCAAGTGTGGTAGTTGCCAAAGCTACCAAGAATCTACCAAACAGCTCACTATGAAGAAGTTACCAG
  TAGTTGCCTGCTTTCATTTCAAACGGTTTGAACACTCAGCGAAACAGAGGCGCAAGATCACTACGTACAT
  TTCCTTTCCTCTGGAGCTGGATATGACACCATTTATGGCGTCAAGTAAAGAGACCCGGGTGAATGGACAG
  TTGCAGCTCCCAACCAATAGTGCGAACAACGAGAATAAGTATTCCTTGTTTGCTGTGGTTAATCACCAAG
  GAACCTTGGAGAGTGGCCACTACACCAGCTTCATTCGGCACCACAGGGACCAGTGGTTCAAGTGTGATGA
  TGCCGTAATCACCAAGGCCAGTATTAAGGATGTGCTGGACAGTGAAGGGTATTTACTGTTCTATCACAAA
  CAGGTCCTGGAACCTGAGCCAGAAAAGGTGAAAGAAATGACTCCACAAGCCTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019461
• Insert Size: 1317 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019461.4, NP_062334.2
• RefSeq Size: 3240 bp
• RefSeq ORF: 1317 bp
• Locus ID: 54651
• UniProt ID: Q8CEG8
• Cytogenetics: X 3.31 cM
• Gene Summary: Deubiquitinase that can reduce the levels of BCL2L11/BIM ubiquitination and stabilize BCL2L11 in response to the RAF-MAPK-degradation signal. By acting on BCL2L11 levels, may counteract the anti-apoptotic effects of MAPK activity.[UniProtKB/Swiss-Prot Function]