Tac1 (NM_009311) Mouse Tagged ORF Clone

CAT#: MG226087

Tac1 (tGFP-tagged) - Mouse tachykinin 1 (Tac1), (10ug)

Product Images

Circular map for MG226087

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tac1
• Synonyms: 4930528L02Rik; NK-1; NK1; Nkna; PPT-A; PPTA; SP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226087 representing NM_009311
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATCCTCGTGGCCGTGGCGGTCTTTTTTCTCGTTTCCACTCAACTGTTTGCAGAGGAAATCGATG
  CCAACGATGATCTAAATTATTGGTCCGACTGGTCCGACAGTGACCAGATCAAGGAGGCAATGCCGGAGCC
  CTTTGAGCATCTTCTGCAGAGAATCGCCCGAAGACCCAAGCCTCAGCAGTTCTTTGGATTAATGGGCAAG
  CGGGATGCTGATTCCTCAGTTGAAAAACAAGTGGCCCTGTTAAAGGCTCTTTATGGACATGGCCAGATCT
  CTCACAAAAGGCATAAAACAGATTCCTTTGTTGGACTAATGGGCAAAAGAGCTTTAAATTCTGTGGCTTA
  TGAAAGAAGCGCGATGCAGAACTACGAAAGAAGACGTAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226087 representing NM_009311
  Red=Cloning site Green=Tags(s)
  
  MKILVAVAVFFLVSTQLFAEEIDANDDLNYWSDWSDSDQIKEAMPEPFEHLLQRIARRPKPQQFFGLMGK
  RDADSSVEKQVALLKALYGHGQISHKRHKTDSFVGLMGKRALNSVAYERSAMQNYERRRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009311
• ORF Size: 390 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009311.3
• RefSeq Size: 1034 bp
• RefSeq ORF: 393 bp
• Locus ID: 21333
• UniProt ID: P41539
• Cytogenetics: 6 3.31 cM
• Gene Summary: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.[UniProtKB/Swiss-Prot Function]