Tle4 (NM_011600) Mouse Tagged ORF Clone

CAT#: MG225876

Tle4 (tGFP-tagged) - Mouse transducin-like enhancer of split 4 homolog of Drosophila E(spl) (Tle4), (10ug)

Product Images

Circular map for MG225876

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tle4
• Synonyms: 5730411M05Rik; AA792082; Bce-1; Bce1; ESTM13; ESTM14; grg-4; Grg4; X83332; X83333
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225876 representing NM_011600
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCGCGACCTGAGCAAGATGTACCCGCAGACGCGCCACCCGGCACCGCATCAGCCTGCTCAACCCT
  TCAAATTTACAATTTCAGAATCCTGTGATCGGATTAAGGAAGAGTTTCAGTTTTTACAGGCTCAATACCA
  CAGTCTGAAGCTGGAATGTGAGAAGCTCGCCAGCGAGAAGACAGAGATGCAGCGGCATTATGTCATGTAT
  TATGAAATGTCCTATGGGTTGAACATAGAAATGCACAAGCAGGCAGAGATTGTTAAACGACTAAATGCTA
  TCTGTGCACAGGTCATTCCTTTCCTGTCCCAAGAGCACCAGCAACAAGTGGTGCAGGCTGTGGAACGGGC
  CAAGCAGGTGACCATGGCAGAACTGAACGCCATCATTGGGCAACAACTCCAAGCTCAGCATTTATCACAT
  GGACATGGTCTGCCTGTGCCTCTGACACCACACCCTTCAGGGCTTCAGCCCCCAGCCATCCCACCTATCG
  GTAGCAGTGCAGGACTTCTGGCCCTCTCCAGTGCACTAGGAGGTCAGTCTCACCTCCCAATTAAGGATGA
  GAAGAAGCACCATGACAATGATCACCAAAGAGACAGAGACTCCATCAAGAGCTCTTCCGTATCCCCATCA
  GCCAGTTTCCGGGGTTCTGAGAAACATAGGAACTCTACAGACTATTCCTCAGAGAGCAAAAAGCAGAAAA
  CAGAAGAAAAAGAAATTGCAGCTCGCTATGACAGTGATGGTGAGAAGAGTGATGACAACTTGGTAGTTGA
  TGTGTCCAATGAGGATCCATCTTCCCCCCGAGGAAGCCCAGCACATTCCCCAAGGGAGAATGGCCTGGAC
  AAGACACGACTCCTCAAGAAAGATGCCCCCATCAGCCCCGCTTCTGTTGCATCTTCCAGCAGTACTCCTT
  CCTCCAAATCCAAAGAGCTTAGCCTTAATGAAAAGTCTACTACTCCTGTTTCAAAGTCCAATACCCCTAC
  TCCACGAACTGATGCACCTACCCCTGGCAGTAATTCTACTCCTGGACTGAGGCCTGTACCTGGAAAGCCA
  CCAGGTGTGGACCCCTTAGCATCAAGCCTGAGGACCCCGATGGCTGTACCTTGTCCATATCCAACCCCCT
  TTGGGATCGTGCCCCATGCTGGGATGAACGGAGAGCTGACCAGCCCCGGAGCCGCCTATGCTGGGCTACA
  CAACATCTCCCCCCAGATGAGTGCAGCTGCTGCTGCAGCTGCGGCAGCAGCAGCCTATGGAAGATCACCT
  GTGGTGGGATTTGATCCACACCATCACATGCGAGTGCCAGCAATACCTCCAAATCTAACAGGCATTCCAG
  GAGGAAAACCAGCATACTCCTTTCACGTCAGTGCCGATGGCCAGATGCAGCCTGTCCCTTTCCCCCCTGA
  TGCCCTCATTGGACCAGGGATCCCCCGACACGCTCGACAGATCAACACCCTCAACCATGGGGAGGTGGTG
  TGTGCAGTGACCATCAGCAACCCCACAAGGCACGTGTACACAGGTGGCAAGGGCTGTGTTAAGGTCTGGG
  ACATCAGCCACCCTGGCAACAAGAGCCCAGTCTCTCAGCTGGACTGTCTGAACAGGGATAACTACATCCG
  TTCCTGCAGATTGCTCCCTGATGGTCGCACCTTAATTGTTGGAGGGGAAGCCAGCACACTGTCCATCTGG
  GACCTGGCAGCTCCAACTCCACGCATCAAGGCAGAGCTGACATCCTCAGCCCCTGCCTGCTATGCTCTGG
  CCATCAGCCCCGACTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGTAACATCGCAGTGTGGGATCTGCA
  CAACCAGACTCTGGTGAGGCAATTCCAGGGGCACACAGATGGAGCCAGCTGTATTGACATTTCTAATGAT
  GGCACCAAGCTCTGGACAGGTGGTTTGGACAACACTGTGAGGTCCTGGGACCTGCGTGAAGGGCGGCAGC
  TGCAGCAACATGACTTCACCTCTCAGATCTTTTCATTGGGCTATTGCCCAACTGGAGAGTGGCTTGCAGT
  GGGGATGGAGAATAGCAATGTGGAAGTATTGCATGTCACCAAACCAGACAAATACCAGTTGCATCTTCAT
  GAGAGCTGTGTGCTGTCACTCAAGTTTGCCCACTGTGGCAAATGGTTTGTAAGCACTGGAAAGGACAACC
  TTCTGAATGCTTGGAGGACGCCTTATGGGGCCAGCATATTCCAGTCCAAAGAATCCTCATCGGTGCTTAG
  CTGTGACATCTCTGTGGATGACAAGTACATTGTCACTGGCTCTGGGGACAAGAAAGCTACGGTTTATGAA
  GTTATTTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225876 representing NM_011600
  Red=Cloning site Green=Tags(s)
  
  MIRDLSKMYPQTRHPAPHQPAQPFKFTISESCDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMY
  YEMSYGLNIEMHKQAEIVKRLNAICAQVIPFLSQEHQQQVVQAVERAKQVTMAELNAIIGQQLQAQHLSH
  GHGLPVPLTPHPSGLQPPAIPPIGSSAGLLALSSALGGQSHLPIKDEKKHHDNDHQRDRDSIKSSSVSPS
  ASFRGSEKHRNSTDYSSESKKQKTEEKEIAARYDSDGEKSDDNLVVDVSNEDPSSPRGSPAHSPRENGLD
  KTRLLKKDAPISPASVASSSSTPSSKSKELSLNEKSTTPVSKSNTPTPRTDAPTPGSNSTPGLRPVPGKP
  PGVDPLASSLRTPMAVPCPYPTPFGIVPHAGMNGELTSPGAAYAGLHNISPQMSAAAAAAAAAAAYGRSP
  VVGFDPHHHMRVPAIPPNLTGIPGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVV
  CAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCRLLPDGRTLIVGGEASTLSIW
  DLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISND
  GTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMENSNVEVLHVTKPDKYQLHLH
  ESCVLSLKFAHCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYE
  VIY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011600
• ORF Size: 2319 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011600.3, NP_035730.2
• RefSeq Size: 4555 bp
• RefSeq ORF: 2322 bp
• Locus ID: 21888
• UniProt ID: Q62441
• Cytogenetics: 19 9.11 cM
• Gene Summary: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression.[UniProtKB/Swiss-Prot Function]