Limk1 (NM_010717) Mouse Tagged ORF Clone
CAT#: MG225384
- TrueORF®
Limk1 (tGFP-tagged) - Mouse LIM-domain containing protein kinase (Limk1), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_010717" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Limk1 |
Synonyms | KIZ-1; Limk; LIMK-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG225384 representing NM_010717
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTTGACGCTACTTTGTTGCACCTGGAGGGAAGAACGTATGGGAGAGGAAGGAAGCGAGTTGCCTG TGTGTGCGAGCTGTGGCCAGAGGATCTATGACGGCCAGTACCTCCAGGCCCTGAATGCTGACTGGCATGC AGACTGCTTCAGGTGCTGCGAGTGTAGCGTCTCCCTTTCACACCAGTACTACGAGAAGGATGGACAGCTC TTCTGCAAGAAGGACTACTGGGCCCGCTATGGCGAGTCTTGCCACGGGTGCTCCGAGCACATCACCAAAG GGCTGGTCATGGTGGCTGGGGAGCTGAAGTACCACCCTGAGTGTTTCATCTGCCTCGCCTGTGGAAACTT CATTGGCGATGGGGACACCTACACACTGGTGGAGCACTCCAAGCTGTACTGTGGCCAGTGCTACTACCAG ACCGTGGTAACTCCAGTCATCGAACAGATCCTACCTGACTCACCCGGCTCCCACCTGCCCCACACAGTCA CCCTCGTGTCTATCCCAGCCTCTGCCCATGGCAAACGAGGCCTGTCTGTCTCCATCGACCCCCCTCACGG CCCACCAGGATGTGGCACGGAGCATTCGCACACTGTCCGAGTCCAAGGAGTGGACCCAGGCTGCATGAGC CCAGATGTGAAGAATTCCATCCACGTTGGAGACCGGATCTTGGAAATCAATGGCACGCCCATCCGGAATG TGCCGCTGGACGAGATCGACTTGCTGATCCAGGAGACCAGCCGCCTGCTCCAGCTGACCCTCGAGCACGA CCCCCATGACTCACTGGGCCATGGGCCCGTGTCGGATCCCAGCCCCCTGAGCTCCCCGGTGCACACTCCC AGCGGACAGGCAGCCAGCTCTGCCCGGCAGAAACCTGTCTTGAGGAGCTGCAGTATTGACACGTCCCCGG GTACCAGCTCACTAGCCTCTCCAGCCTCCCAGCGCAAGGACCTGGGTCGCTCCGAATCCCTCCGAGTGGT CTGCCGACCACACCGAATCTTCCGGCCATCTGATCTCATCCATGGGGAAGTGCTGGGCAAGGGCTGTTTT GGCCAGGCCATCAAGGTGACACACCGAGAAACAGGCGAGGTGATGGTGATGAAGGAACTTATCCGGTTTG ATGAGGAGACCCAGAGGACATTCCTCAAGGAGGTAAAGGTCATGCGCTGCCTGGAGCACCCTAACGTGCT CAAGTTCATCGGAGTGCTCTACAAGGACAAGCGGCTAAACTTCATCACAGAGTACATCAAGGGCGGCACC CTGCGGGGCATCATCAAGAACATGGACAGCCAGTACCCGTGGAGTCAGAGGGTCAGCTTTGCCAAGGACA TTGCATCAGGGATGGCCTACCTCCATTCGATGAACATCATCCACCGAGACCTCAACTCCCACAACTGTCT GGTCCGTGAGAATAGGAACGTGGTGGTGGCCGACTTTGGGCTGGCTCGACTCATGATCGATGAAAAGAAT CAGTCTGAAGACTTACGCAGCCTTAAGAAGCCGGACCGCAAGAAGCGGTACACAGTGGTGGGCAACCCCT ACTGGATGGCGCCAGAGATGATCAATGGTCGTAGTTATGATGAAAAGGTGGACGTGTTTTCCTTTGGAAT TGTCCTCTGTGAGATTATCGGGCGTGTGAATGCAGACCCTGACTATCTGCCCCGCACTATGGACTTTGGC CTCAATGTAAGGGGTTTCCTGGACCGCTACTGTCCACCAAACTGTCCCCCAAGTTTCTTCCCCATCACTG TGCGCTGCTGTGACCTGGATCCTGAAAAGAGACCCTCTTTTGTAAAGCTGGAACAATGGCTAGAAACACT TCGCATGCACTTGTCTGGTCACCTGCCACTGGGCCCACAACTGGAACAACTGGAGCGGGGCTTCTGGGAG ACCTACCGGCGGGGCGAGAGCTCACTGCCTGCCCACCCCGAGGTCCCTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225384 representing NM_010717
Red=Cloning site Green=Tags(s) MRLTLLCCTWREERMGEEGSELPVCASCGQRIYDGQYLQALNADWHADCFRCCECSVSLSHQYYEKDGQL FCKKDYWARYGESCHGCSEHITKGLVMVAGELKYHPECFICLACGNFIGDGDTYTLVEHSKLYCGQCYYQ TVVTPVIEQILPDSPGSHLPHTVTLVSIPASAHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMS PDVKNSIHVGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDSLGHGPVSDPSPLSSPVHTP SGQAASSARQKPVLRSCSIDTSPGTSSLASPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCF GQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGT LRGIIKNMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENRNVVVADFGLARLMIDEKN QSEDLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFG LNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEQWLETLRMHLSGHLPLGPQLEQLERGFWE TYRRGESSLPAHPEVPD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010717 |
ORF Size | 1941 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010717.3, NP_034847.1 |
RefSeq Size | 3323 bp |
RefSeq ORF | 1944 bp |
Locus ID | 16885 |
UniProt ID | P53668 |
Cytogenetics | 5 G2 |
Gene Summary | This gene encodes a member of the LIM kinase family of proteins. This protein is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein also stimulates axon growth and may play a role in brain development. Homozygous knockout mice for this gene exhibit reduced bone mass, abnormal neuronal morphology and altered synaptic function. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220064 | Limk1 (untagged) - Mouse LIM-domain containing, protein kinase (Limk1), (10ug) |
USD 663.00 |
|
MR225384 | Limk1 (Myc-DDK-tagged) - Mouse LIM-domain containing, protein kinase (Limk1) |
USD 603.00 |
|
MR225384L3 | Lenti ORF clone of Limk1 (Myc-DDK-tagged) - Mouse LIM-domain containing, protein kinase (Limk1) |
USD 903.00 |
|
MR225384L4 | Lenti ORF clone of Limk1 (mGFP-tagged) - Mouse LIM-domain containing, protein kinase (Limk1) |
USD 903.00 |
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