Opn5 (NM_181753) Mouse Tagged ORF Clone
CAT#: MG224963
- TrueORF®
Opn5 (tGFP-tagged) - Mouse opsin 5 (Opn5), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_181753" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Opn5 |
Synonyms | Gpr136; Neuropsin; PGR12; TMEM13 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224963 representing NM_181753
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGAACCACACTGCCCTACCTCAGGATGAGCGCCTGCCCCACTATCTTCGAGACGAGGACCCTT TTGCTTCCAAACTTTCCTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTAACAATAATCGGGATTCTCTC TACATTTGGAAATGGGTATGTCCTTTATATGTCTTCTAGACGCAAGAAGAAGCTGAGACCTGCGGAAATA ATGACTATCAATTTAGCAGTCTGTGATCTGGGGATATCAGTTGTAGGCAAGCCGTTCACCATCATCTCTT GCTTCTGCCACCGCTGGGTGTTTGGCTGGTTTGGCTGCCGCTGGTATGGCTGGGCTGGATTTTTCTTTGG CTGTGGAAGCCTGATTACCATGACTGCTGTCAGCCTGGACCGCTATCTGAAGATCTGTTATCTGTCTTAT GGGGTCTGGCTGAAGAGAAAGCATGCCTACATCTGCCTGGCAGTCATCTGGGCTTATGCTTCCTTCTGGA CCACCATGCCCTTGGTGGGCCTGGGGGACTATGCACCTGAGCCCTTCGGAACCTCATGCACCCTGGACTG GTGGCTGGCCCAGGCTTCAGGTGGGGGTCAGGTGTTCATCCTGAGCATCCTCTTCTTCTGCCTCCTGCTG CCAACGGCTGTGATTGTTTTCTCATATGCTAAGATCATCGCCAAGGTGAAGTCTTCTTCTAAAGAGGTAG CCCATTTCGACAGTCGAATCCATAGCAGCCATGTACTTGAGGTGAAGCTGACCAAGGTGGCAATGCTGAT TTGTGCTGGTTTCCTGATTGCCTGGATTCCTTATGCGGTCGTCTCTGTGTGGTCAGCTTTTGGAAGGCCA GACTCCATCCCCATACAGCTCTCCGTGGTGCCCACACTCCTTGCAAAATCAGCAGCGATGTACAATCCAA TCATCTACCAGGTCATTGATTACAGATTTGCCTGTTGCCAGGCTGGTGGTTTGAGAGGAACGAAGAAGAA ATCTTTGGAAGACTTTAGGCTGCATACTGTAACCGCAGTCAGGAAGTCTTCTGCTGTGCTGGAGATCCAT CCAGAGAGCAGTTCCAGATTTACTAGTGCCCATGTTATGGATGGAGAGAGTCACAGTAATGATGGTGACT GTGGCAAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224963 representing NM_181753
Red=Cloning site Green=Tags(s) MALNHTALPQDERLPHYLRDEDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEI MTINLAVCDLGISVVGKPFTIISCFCHRWVFGWFGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASGGGQVFILSILFFCLLL PTAVIVFSYAKIIAKVKSSSKEVAHFDSRIHSSHVLEVKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRP DSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYRFACCQAGGLRGTKKKSLEDFRLHTVTAVRKSSAVLEIH PESSSRFTSAHVMDGESHSNDGDCGKK TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_181753 |
ORF Size | 1131 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181753.4, NP_861418.2 |
RefSeq Size | 1824 bp |
RefSeq ORF | 1134 bp |
Locus ID | 353344 |
UniProt ID | Q6VZZ7 |
Cytogenetics | 17 B3 |
Gene Summary | G-protein coupled receptor which selectively activates G(i) type G proteins via ultraviolet A (UVA) light-mediated activation in the retina (PubMed:22043319). Preferentially binds the chromophore 11-cis retinal and is a bistable protein that displays emission peaks at 380 nm (UVA light) and 470 nm (blue light) (PubMed:22043319, PubMed:31607531). Required for the light-response in the inner plexiform layer, and contributes to the regulation of the light-response in the nerve fiber layer, via phosphorylated DAT/SLC6A3 dopamine uptake (PubMed:30936473). Involved in local corneal and retinal circadian rhythm photoentrainment via modulation of the UVA light-induced phase-shift of the retina clock (PubMed:26392540, PubMed:30240620). Acts as a circadian photoreceptor in the outer ear and vibrissal pads, via modulation of circadian clock-gene expression in response to violet light during the light-to-dark transition phase and night phase of the circadian cycle (PubMed:31607531). Required in the retina to negatively regulate hyaloid vessel regression during postnatal development via light-dependent OPN5-SLC32A1-DRD2-VEGFR2 signaling (PubMed:30936473). Involved in the light-dependent regulation of retina and vitreous compartment dopamine levels (PubMed:30936473).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC214652 | Opn5 (untagged) - Mouse opsin 5 (Opn5), (10ug) |
USD 457.00 |
|
MR224963 | Opn5 (Myc-DDK-tagged) - Mouse opsin 5 (Opn5) |
USD 457.00 |
|
MR224963L3 | Lenti ORF clone of Opn5 (Myc-DDK-tagged) - Mouse opsin 5 (Opn5) |
USD 757.00 |
|
MR224963L4 | Lenti ORF clone of Opn5 (mGFP-tagged) - Mouse opsin 5 (Opn5) |
USD 757.00 |
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