Kcng4 (NM_025734) Mouse Tagged ORF Clone

CAT#: MG224440

Kcng4 (tGFP-tagged) - Mouse potassium voltage-gated channel subfamily G member 4 (Kcng4), (10ug)

Product Images

Circular map for MG224440

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kcng4
• Synonyms: 4921535I01Rik; AW049024; KV6.3; KV6.4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224440 representing NM_025734
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATGTCTTCCAGAGACAGGGACTTGCATCCTGGACACCATCACTTTGGCTCCTGCAGCCCCTTGA
  GCCAGCTCTGGCCGGGCCCCGAGCCTAAGTCAGTCAAGGGCCTTTACTACAGCAGGGCCCGGAAGGTGGG
  CAACCAGGACGCCTCTCCGGAGGCCAACTTGAAGGAGATCCTAGTGAATGTGGGTGGCCAGCGGTACCTG
  CTGCCCTGGAGCACCCTGGATGCCTTCCCGCTGAGCCGCCTGAGCAGGCTCCGGCTGTGCCGCAGCCATG
  AGGAGATCACGCAGCTCTGCGATGACTACGATGAGGACAGCCAGGAGTTCTTCTTCGACAGGAACCCCAG
  CGCCTTCGGGGTGATCGTGAGCTTCCTGGCCGCGGGAAAGCTGGTGCTTCTGCGAGAGATGTGCGCCCTG
  TCCTTCCGGGAGGAGCTGAGCTACTGGGGCATCGAGGAAACCAACCTGGAGCGCTGCTGCCTGCGCAAGC
  TGCTGAAGAAGCTGGAGGAGGCGGCCGAGCTGCGCCGGGAGGAGGCTGCCCAGCGCCAGCAGCAGCGCCA
  GGCCTGCCACTCCGAGGTGCAGGCTTCACGATGGGCCCGCAGCATGAACCAGCTGCGTGAAATGGTGGAG
  GACCCACAGTCGGGGCTGCCCGGGAAGGTCTTCGCCTGCCTCTCCGTGCTCTTCGTGGCAACCACGGCTG
  TCAGCCTGTGTGTGAGCACCATGCCCGACTTCAGGGCTGAGGAGGGCAAGGGAGAATGCACTAGAAAGTG
  CTATTACATCTTCGTGGTGGAATCCATCTGTGTGGCCTGGTTCTCGCTGGAGTTTTGCCTGCGCTTTGTC
  CAGGCCCCGAACAAATGTCAGTTCTTCCGCGGGCCCCTGAATGTCATCGACATTCTAGCCATCTCCCCAT
  ACTATGTGTCGCTCGCAGTGTCTGACGAATCCCCGGAGGCAGGCGAGAGGCCGAGCAGCAGCTCCTACCT
  GGAGAAAGTGGGGTTAGTGCTGCGTGTTCTGCGGGCACTACGCATCCTCTACGTAATGCGCCTGGCTCGC
  CACTCCCTGGGGCTGCAGACGCTGGGCCTCACTGTGCGCCGCTGCGCCCGAGAGTTTGGTCTCCTGATGC
  TCTTCCTGGCTGTGGCGGTTACCCTCTTCTCACCGTTGGTCTATGTAGCTGAGAATGAGTCCGGAAGGGT
  CCTGGAGTTCACTAGCATCCCCGCATCCTATTGGTGGGCCATCATCTCCATGACGACCGTGGGCTATGGG
  GACATGGTCCCTCGCAGCGTCCCGGGACAGATGGTGGCTCTGAGCAGCATCCTTAGCGGGATCCTTATCA
  TGGCTTTCCCAGCCACATCCATCTTCCACACGTTCTCTCACTCCTACCTGGAGCTGAAGCGGGAGCAGGA
  GCAGGTCCAGGCCCGCCTCCGGCGTCTTCAGAACACCAATTCGGCCAGCGAACGGGAGCTCCTGAGTGAC
  GTAGATGATCTGGTCCCTGAGGGTCTGACCTCCCCAGGCCGCTACATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224440 representing NM_025734
  Red=Cloning site Green=Tags(s)
  
  MPMSSRDRDLHPGHHHFGSCSPLSQLWPGPEPKSVKGLYYSRARKVGNQDASPEANLKEILVNVGGQRYL
  LPWSTLDAFPLSRLSRLRLCRSHEEITQLCDDYDEDSQEFFFDRNPSAFGVIVSFLAAGKLVLLREMCAL
  SFREELSYWGIEETNLERCCLRKLLKKLEEAAELRREEAAQRQQQRQACHSEVQASRWARSMNQLREMVE
  DPQSGLPGKVFACLSVLFVATTAVSLCVSTMPDFRAEEGKGECTRKCYYIFVVESICVAWFSLEFCLRFV
  QAPNKCQFFRGPLNVIDILAISPYYVSLAVSDESPEAGERPSSSSYLEKVGLVLRVLRALRILYVMRLAR
  HSLGLQTLGLTVRRCAREFGLLMLFLAVAVTLFSPLVYVAENESGRVLEFTSIPASYWWAIISMTTVGYG
  DMVPRSVPGQMVALSSILSGILIMAFPATSIFHTFSHSYLELKREQEQVQARLRRLQNTNSASERELLSD
  VDDLVPEGLTSPGRYM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025734
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025734.2, NP_080010.2
• RefSeq Size: 3822 bp
• RefSeq ORF: 1521 bp
• Locus ID: 66733
• UniProt ID: Q80XM3
• Cytogenetics: 8 E1
• Gene Summary: Potassium channel subunit that does not form functional channels by itself. Can form functional heterotetrameric channels with KCNB1; modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1.[UniProtKB/Swiss-Prot Function]